發(fā)布時(shí)間：2018-03-11 13:04

  本文選題：普通小麥　切入點(diǎn)：近緣種　出處：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：低分子量麥谷蛋白約占小麥種子貯藏蛋白的三分之一,對(duì)面團(tuán)延展性和食品加工品質(zhì)有著重要影響。普通小麥及其近緣種是小麥遺傳改良的重要基因資源。本研究利用小麥Glu-A3和Glu-B3基因的特異引物對(duì)強(qiáng)筋型、中筋型和弱筋型小麥以及小麥近緣種共計(jì)21份材料的LMW-GS基因進(jìn)行擴(kuò)增和鑒定,共計(jì)發(fā)現(xiàn)了33個(gè)新的核苷酸變異類型和26個(gè)肽鏈變異類型,包括在GluA-3位點(diǎn)上得到19個(gè)GluA-3基因變異類型和19個(gè)對(duì)應(yīng)的肽鏈變異類型,在GluB-3位點(diǎn)上得到5個(gè)Glu-B3基因變異類型和3個(gè)肽鏈變異類型,在GluD-3位點(diǎn)上得到9個(gè)Glu-D3基因變異類型和4個(gè)肽鏈變異類型。此外,還發(fā)現(xiàn)3個(gè)半胱氨酸數(shù)目特殊的亞基類型,分別是來自品種師欒02-1和栽培二粒小麥DM2含有9個(gè)半胱氨酸殘基的GluA3-18基因、野生一粒小麥B03含有9個(gè)半胱氨酸殘基的GluA3-312基因以及偃展4110含有7個(gè)半胱氨酸殘的GluD3-13基因。3個(gè)半胱氨酸數(shù)目特殊的亞基類型,可能屬于優(yōu)良品質(zhì)亞基。對(duì)普通小麥及其近緣種中這些Glu-3位點(diǎn)等位變異的鑒別,進(jìn)一步完善了小麥低分子量麥谷蛋白亞基的構(gòu)成,并為小麥品質(zhì)育種中親本的選擇提供了相應(yīng)依據(jù)。
[Abstract]:Low molecular weight glutenin accounts for about 1/3 of wheat seed storage protein. The ductility and food processing quality of the opposite group have an important effect on wheat. The common wheat and its relative species are important genetic resources for wheat genetic improvement. In this study, the strong gluten type was identified by using the specific primers of Glu-A3 and Glu-B3 genes in wheat. The LMW-GS gene of 21 accessions of middle gluten type and weak gluten type wheat and their relative species were amplified and identified. A total of 33 new nucleotide variation types and 26 peptide chain variant types were found. 19 GluA-3 gene variants and 19 corresponding peptide chain variants were obtained at GluA-3 loci, and 5 Glu-B3 gene variants and 3 peptide chain variant types were obtained at GluB-3 loci. Nine Glu-D3 gene variants and four peptide chain variants were obtained at GluD-3 loci. In addition, three specific subunit types of cysteine were found. The GluA3-18 gene from the cultivar Luan Luan 02-1 and the cultivated wheat DM2 contains 9 cysteine residues, respectively. Wild wheat B03 contains 9 cysteine residues of GluA3-312 gene and Yanchan 4110 contains 7 cysteine residues of GluD3-13 gene. The allelic variation of these Glu-3 loci in common wheat and its relative species was identified, which further improved the composition of wheat low molecular weight glutenin subunits. It also provides the basis for the selection of parents in wheat quality breeding.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S512.1

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