本文選題：番茄　切入點(diǎn)：生長(zhǎng)素　出處：《江蘇農(nóng)業(yè)科學(xué)》2017年05期 　論文類(lèi)型：期刊論文

【摘要】：Aux/IAA(auxin/indole-3-acetic acid)作為生長(zhǎng)素早期響應(yīng)因子,其蛋白產(chǎn)物能夠特異性地結(jié)合生長(zhǎng)素響應(yīng)因子(auxin response factor,ARF),從而調(diào)控生長(zhǎng)素響應(yīng)基因的表達(dá),在整個(gè)植物生長(zhǎng)素信號(hào)轉(zhuǎn)導(dǎo)過(guò)程中發(fā)揮著重要的作用,進(jìn)而影響植物的生長(zhǎng)發(fā)育。本研究通過(guò)構(gòu)建沉默載體,在高效遺傳轉(zhuǎn)化體系下獲得轉(zhuǎn)基因植株,發(fā)現(xiàn)利用Gateway克隆技術(shù)將番茄生長(zhǎng)素早期響應(yīng)基因Sl IAA16 DomainⅡ區(qū)部分序列連入具有正反2個(gè)attr區(qū)域并連接1個(gè)內(nèi)含子的p B7GWIWG2(Ⅰ)載體,可在植株體內(nèi)形成發(fā)卡結(jié)構(gòu)導(dǎo)致植物基因沉默,成功構(gòu)建基因沉默表達(dá)載體名為Sl IAA RNAi;此外,抗生素噴施和PCR擴(kuò)增以及實(shí)時(shí)定量檢測(cè)進(jìn)一步鑒定,共獲得12棵Sl IAA16 RNAi陽(yáng)性植株。本研究結(jié)果將為進(jìn)一步明確Sl IAA的生物學(xué)功能和闡明生長(zhǎng)素的作用機(jī)理提供參考。
[Abstract]:Aux/IAA(auxin/indole-3-acetic acid. as an early response factor of auxin, its protein product can specifically bind to auxin response factor of auxin, thus regulating the expression of auxin response gene, which plays an important role in the process of auxin signal transduction. In this study, we constructed silencing vector to obtain transgenic plants in high efficiency genetic transformation system. It was found that the partial sequence of SL IAA16 Domain 鈪，

本文編號(hào)：1596305