本文選題：蜚蠊　切入點(diǎn)：放線菌　出處：《生物技術(shù)》2017年05期 　論文類(lèi)型：期刊論文

【摘要】：[目的]對(duì)159株蜚蠊腸道內(nèi)生放線菌的鹵化酶等相關(guān)基因進(jìn)行檢測(cè)和初步分析。[方法]活化蜚蠊腸道內(nèi)生放線菌,制備各菌株基因組DNA,根據(jù)依賴(lài)黃素腺嘌呤二核苷酸FADH2的鹵化酶基因保守區(qū)設(shè)計(jì)簡(jiǎn)并引物,通過(guò)PCR擴(kuò)增鹵化酶基因片段,TA克隆后進(jìn)行測(cè)序鑒定。在此基礎(chǔ)上,對(duì)鹵化酶陽(yáng)性菌株進(jìn)行聚酮合酶PKSⅠ和非核糖體多肽合成酶NRPS等2個(gè)次級(jí)代謝產(chǎn)物生物合成主要基因進(jìn)行檢測(cè)。[結(jié)果]159株蜚蠊腸道放線菌有84株含有鹵化酶基因,占比52.83%;鹵化酶基因陽(yáng)性放線菌中71株含有PKSⅠ基因,70株含有NRPS,占比分別為84.52%、83.33%。[結(jié)論]蜚蠊腸道含有較豐富的鹵化酶基因陽(yáng)性放線菌,可作為未來(lái)分離鹵化活性物質(zhì)的微生物資源。
[Abstract]:[objective] to detect and analyze the halogenase and other related genes of 159 strains of cockroach intestinal actinomycetes. [methods] Activation of intestinal actinomycetes from cockroaches. The genomic DNAs of each strain were prepared. Degenerate primers were designed according to the conserved region of the halogenase gene dependent on the FADH2 of Flavin adenine dinucleotide. The halogenase gene fragment was cloned by PCR and sequenced. Two secondary metabolite biosynthesis genes, including polyketone synthase (PKS 鈪，

本文編號(hào)：1594801