本文選題：草莓　切入點(diǎn)：蔗糖堿性/中性轉(zhuǎn)化酶　出處：《園藝學(xué)報(bào)》2017年06期 　論文類型：期刊論文

【摘要】：在草莓蔗糖堿性/中性轉(zhuǎn)化酶家族基因(Fa.A/N-Inv)成員序列分析的基礎(chǔ)上,以‘申陽’和‘紅顏’草莓為材料,利用實(shí)時(shí)定量PCR技術(shù)分析了Fa.A/N-Inv家族基因在不同條件下的表達(dá)模式,利用分光光度法測(cè)定了果實(shí)發(fā)育過程中A/N-Inv酶活性,利用高效液相色譜法分析了果實(shí)發(fā)育階段糖分積累規(guī)律。結(jié)果表明:(1)Fa.A/N-Inv是由8個(gè)成員組成的多基因家族;(2)4個(gè)Fa.A/N-Invs不僅具有組織特異性,還具有果實(shí)發(fā)育階段特異性和品種特異性表達(dá)特點(diǎn);(3)4個(gè)Fa.A/N-Invs的表達(dá)受細(xì)胞分裂素(BA)、赤霉素(GA)和生長(zhǎng)素(IAA)誘導(dǎo);(4)蔗糖和葡萄糖作為A/N-Inv酶促反應(yīng)的底物和產(chǎn)物,分別對(duì)4個(gè)Fa.A/N-Invs轉(zhuǎn)錄表達(dá)起正、負(fù)反饋調(diào)節(jié)作用,此外蔗糖還可作為一種信號(hào)分子誘導(dǎo)Fa.A/N-Invs表達(dá);(5)‘申陽’草莓A/N-Inv酶活性在綠果階段低于‘紅顏’,其余階段均高于‘紅顏’;(6)成熟果實(shí)中蔗糖含量‘紅顏’(50.7 mg·g~(-1) FW)約為‘申陽’(23.2 mg·g~(-1) FW)的2倍。
[Abstract]:In the strawberry sucrose alkaline / neutral invertase gene family (Fa.A/N-Inv) based on the analysis of the sequence members, "Shen Yang" and "Confidante" strawberry as materials, analyzed the expression pattern of Fa.A/N-Inv gene family in different conditions by using real-time quantitative PCR technology, using A/N-Inv enzyme activity during the fruit development were determined by Spectrophotometry the method of using high performance liquid chromatography analysis of the accumulation of the development stages of fruit sugar rules. The results showed that: (1) Fa.A/N-Inv is a multi gene family consists of 8 members; (2) 4 Fa.A/N-Invs not only has tissue specificity, but also has the fruit developmental stage specific and species specific expression characteristics; (3 4) expression of Fa.A/N-Invs by cytokinin (BA), gibberellin (GA) and auxin (IAA) induction; (4) sucrose and glucose as the substrate of the enzymatic reaction and A/N-Inv products, respectively for 4 Fa.A/N-Invs expression is negative. Feedback regulation, in addition to sucrose can be used as a signal molecule to induce the expression of Fa.A/N-Invs; (5) the "Shen Yang" strawberry A/N-Inv activity in fruit stage than the 'Confidante', the remaining stages were higher than that of "Confidante"; (6) Confidante 'sucrose content in mature fruits' (50.7 Mg - g~ (-1) FW about "Shen Yang") (23.2 Mg - g~ (-1) FW) 2 times.

【作者單位】： 甘肅農(nóng)業(yè)大學(xué)園藝學(xué)院;上海市農(nóng)業(yè)科學(xué)院林木果樹研究所;
【基金】：上海市農(nóng)業(yè)基礎(chǔ)性研究項(xiàng)目[滬農(nóng)科攻字(2014)第7-2-2號(hào)] 上海市科委基礎(chǔ)研究重點(diǎn)項(xiàng)目(14JC1405400);上海市科委農(nóng)業(yè)科技重點(diǎn)攻關(guān)項(xiàng)目(16391901400)
【分類號(hào)】：S668.4

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