發(fā)布時間：2018-03-08 09:38

  本文選題：類風(fēng)濕關(guān)節(jié)炎　切入點：加味牛蒡子湯　出處：《中國中西醫(yī)結(jié)合雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的觀察加味牛蒡子湯(JWNBZT)對類風(fēng)濕關(guān)節(jié)炎(RA)模型動物TNF-α轉(zhuǎn)基因(TNF-Tg)小鼠關(guān)節(jié)炎癥和關(guān)節(jié)周圍淋巴回流功能的影響。方法將16只清潔級3月齡左右TNF-Tg小鼠按照體重隨機(jī)分為TNF-Tg組和JWNBZT組,每組8只。另取10只同窩對照體重相似的野生型小鼠(WT)作為對照組,JWNBZT組每天以JWNBZT灌胃,對照組和TNF-Tg組每天以等量純凈水灌胃,治療12周后小鼠足底皮下注射吲哚菁綠(ICG)測量小鼠淋巴管回流功能,并取踝關(guān)節(jié)制作石蠟切片進(jìn)行蘇木素-伊紅(HE)染色觀察炎性浸潤和關(guān)節(jié)骨質(zhì)破壞情況,抗酒石酸酸性磷酸酶(TRAP)染色觀察破骨細(xì)胞數(shù)量。結(jié)果與對照組比較,TNF-Tg組小鼠淋巴管回流功能出現(xiàn)明顯損害,Clearance和Pulse明顯下降,淋巴管滲漏面積增多,踝關(guān)節(jié)內(nèi)出現(xiàn)明顯炎癥浸潤且骨質(zhì)減少,距骨內(nèi)破骨細(xì)胞增多(P0.05);與TNF-Tg組比較,JWNBZT組小鼠淋巴管回流功能明顯增強(qiáng),Clearance和Pulse升高,淋巴管滲漏面積減少,踝關(guān)節(jié)內(nèi)炎癥浸潤減輕且骨質(zhì)增多,距骨內(nèi)破骨細(xì)胞減少(P0.05)。結(jié)論 JWNBZT能夠減輕RA受累關(guān)節(jié)的炎癥浸潤和關(guān)節(jié)骨質(zhì)破壞,并促進(jìn)淋巴管回流功能,這可能是其治療RA的機(jī)制。
[Abstract]:Objective to observe the effect of JWNBZT (JWNBZT) on arthritis and peripheral lymphoid reflux in TNF- 偽 transgenic TNF-Tg mice. Methods Sixteen TNF-Tg mice of 3 months old of clean grade were selected to follow their body weight according to their body weight. The machine was divided into TNF-Tg group and JWNBZT group. Eight mice in each group were taken as control group, and 10 wild type mice with similar body weight were taken as control group. JWNBZT group was treated with JWNBZT daily, and the control group and TNF-Tg group were treated with the same amount of pure water daily. After 12 weeks of treatment, the subcutaneous injection of indocyanine green (ICG) into the plantar of mice was used to measure the function of lymphatic reflux in mice, and paraffin sections were taken from the ankle joint to be stained with hematoxylin and eosin (HEH) to observe the inflammatory infiltration and joint bone destruction. The number of osteoclasts was observed by tartrate-resistant acid phosphatase (TRAP) staining. Results compared with the control group, the lymphatic reflux function of TNF-Tg group decreased significantly, and the lymphatic vessel leakage area increased. Significant inflammatory infiltration and osteopenia were observed in the ankle joint, and osteoclasts increased in the talus (P 0.05). Compared with the TNF-Tg group, the lymphatic reflux function of the JWNBZT group was significantly increased, and the lymphatic vessel leakage area was decreased in the JWNBZT group, and the function of lymphatic reflux was significantly increased in the JWNBZT group. Conclusion JWNBZT can reduce inflammatory infiltration and joint bone destruction and promote lymphatic reflux function, which may be the mechanism of JWNBZT in the treatment of RA.
【作者單位】： 上海中醫(yī)藥大學(xué)附屬龍華醫(yī)院骨傷科;上海中醫(yī)藥大學(xué)脊柱病研究所;
【基金】：國家自然科學(xué)基金重點項目(No.81330085);國家自然科學(xué)基金重大國際合作項目(No.81220108027);國家自然科學(xué)基金面上項目(No.81173278);國家自然科學(xué)基金青年科學(xué)基金項目(No.81403417,No.81403418) 國家教育部“創(chuàng)新團(tuán)隊發(fā)展計劃”(No.1RT1270) 全國優(yōu)秀博士學(xué)位論文作者專項資助(No.201276)
【分類號】：R285.5

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