本文選題：長春花萜類吲哚生物堿　切入點：合成途徑　出處：《基因組學(xué)與應(yīng)用生物學(xué)》2017年03期 　論文類型：期刊論文

【摘要】：長春花(Catharanthus roseus)中含有豐富的次生代謝產(chǎn)物,可產(chǎn)生130多種萜類吲哚生物堿,包括目前臨床應(yīng)用最廣的天然植物抗癌藥物長春堿(vinblastine)和長春新堿(vincristine)。長春花萜類吲哚生物堿(terpenoid indole alkaloids,TIAs)合成途徑包括在內(nèi)韌皮部相關(guān)薄壁細(xì)胞、上皮細(xì)胞、異細(xì)胞和乳管細(xì)胞等多種組織細(xì)胞內(nèi)進(jìn)行的20多步酶促反應(yīng)。本研究選取TIAs途徑上的CPR、LAMT、SLS、STR、SGD、TDC、16-OMT、T16H、D4H等9個酶基因,基于長春花轉(zhuǎn)錄組數(shù)據(jù)庫,分析其在各組織器官和MeJA處理下的無菌苗、毛狀根和懸浮細(xì)胞中的數(shù)字表達(dá)譜,找出其中可能對MeJA信號響應(yīng)的基因。本研究發(fā)現(xiàn),MeJA處理后,長春花無菌苗中以上9個基因表達(dá)量均發(fā)生上調(diào);毛狀根中除了TDC,另外8個基因表達(dá)豐度均為上調(diào);懸浮細(xì)胞中除了D4H,另外8個基因表達(dá)豐度均上調(diào)。對整個轉(zhuǎn)錄組數(shù)據(jù)進(jìn)行分析,發(fā)現(xiàn)經(jīng)過MeJA處理后,僅有673條Unigene能在無菌苗、毛狀根和懸浮細(xì)胞中均表現(xiàn)出差異表達(dá),說明無菌苗、毛狀根和懸浮細(xì)胞的MeJA應(yīng)答機(jī)制存在較大不同。對這些能在3種體系中均發(fā)生差異表達(dá)的基因進(jìn)行功能分類,主要包括初生代謝相關(guān)蛋白、次生代謝相關(guān)蛋白、植物激素相關(guān)蛋白、礦質(zhì)離子結(jié)合蛋白、轉(zhuǎn)運蛋白、信號轉(zhuǎn)導(dǎo)相關(guān)蛋白、細(xì)胞壁合成與降解相關(guān)蛋白、轉(zhuǎn)錄因子、植物逆境響應(yīng)蛋白9個方面。本研究可為通過外源因子調(diào)控長春花生物堿的生物合成提供參考。
[Abstract]:Catharanthus roseus, which contains abundant secondary metabolites, can produce more than 130 kinds of terpenoid indole alkaloids. Including vinblastine (vinblastine) and vinblastine (vinblastine) and vincristine (vinblastine), the synthetic pathway of terpenoid indole alkaloidsof vinblastine (vinblastine) and vinblastine (vinblastine), including phloem related parenchyma cells, epithelial cells, More than 20 steps of enzymatic reactions were carried out in various tissue cells such as xenoblasts and lactobacillus cells. In this study, nine enzyme genes, such as TIAs pathway, were selected to analyze the aseptic vaccine treated with MeJA and tissue organs, based on the transcriptome database of roseus roseus, and nine enzyme genes, such as TDCC16-OMTT1-T16HD4H, were selected. The digital expression profiles in hairy roots and suspension cells were used to identify the genes that might respond to MeJA signal. In this study, it was found that the above nine genes were up-regulated in aseptic seedlings of Catharanthus roseus after treatment with Meja. In hairy roots, except TDC, the other 8 genes were up-regulated, and in suspension cells, except D4H, the other eight genes were up-regulated. By analyzing the whole transcriptional group data, it was found that only 673 Unigene could be expressed in sterile vaccine after MeJA treatment. The differential expression in hairy root and suspension cell indicated that the mechanism of MeJA response in sterile vaccine, hairy root and suspension cell was different. These proteins include primary metabolism-associated proteins, secondary metabolization-related proteins, plant hormone related proteins, mineral ion binding proteins, transporter proteins, signal transduction related proteins, cell wall synthesis and degradation related proteins, transcription factors, This study can be used as a reference for regulating the biosynthesis of alkaloids in roseus roseus by exogenous factors.
【作者單位】： 西南大學(xué)生命科學(xué)學(xué)院;西南大學(xué)—西藏農(nóng)牧學(xué)院藥用植物聯(lián)合研發(fā)中心;重慶科技學(xué)院;
【基金】：國家級大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃項目(201510635011) 國家863計劃(2011AA100605) 新世紀(jì)優(yōu)秀人才支持計劃(NCET-12-0930)項目基金共同資助
【分類號】：Q943.2

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