本文選題：股骨頭壞死　切入點(diǎn)：軟骨　出處：《中國(guó)骨質(zhì)疏松雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的探討ADAMTS-7與ONFH(股骨頭壞死)患者關(guān)節(jié)軟骨退化和影像學(xué)變化的相關(guān)性,并研究其潛在機(jī)制。方法共納入2014年3月至2015年6月我院收治的80例股骨頸骨折(FNF)患者作為研究對(duì)象。根據(jù)檢查結(jié)果將所有患者分為對(duì)照組和ONFH組,對(duì)照組患者X線片檢查均無ONFH和骨關(guān)節(jié)炎OA;ONFH組患者X線片檢查有明確ONFH。根據(jù)ARCO分期標(biāo)準(zhǔn),將ONFH組患者進(jìn)一步分為兩組:A組,ARCO分期II~III期,X線片顯示股骨頭無明顯塌陷;B組,ARCO分期IV期,X線片顯示股骨頭有明顯塌陷。記錄Mankin評(píng)分;免疫組化染色分析ADAMTS-7、TNF-α和磷酸化NF-κB的表達(dá)水平;RTPCR檢測(cè)ADAMTS-7的mRNA水平;Western Blot檢測(cè)ADAMTS-7與COMP的蛋白水平;分析各組指標(biāo)差異,探討ADAMTS-7在ONFH發(fā)病機(jī)制中的潛在作用。結(jié)果各組患者的年齡、性別等基本信息對(duì)比無統(tǒng)計(jì)學(xué)差異(P0.05)。ONFH組患者關(guān)節(jié)軟骨發(fā)生明顯退化,ONFH A組與ONFH B組的Mankin評(píng)分均顯著高于對(duì)照組(P0.05)。ONFH組的ADAMTS-7和TNF-α表達(dá)水平均顯著高于對(duì)照組(P0.05)。ONFH組ADAMTS-7的mRNA水平顯著高于對(duì)照組(P0.05)。Western Blot結(jié)果顯示ADAMTS-7的增加與COMP的降解潛在相關(guān)(P0.05)。此外,ONFH組患者的NF-κB表達(dá)水平顯著高于對(duì)照組(P0.05)。結(jié)論 ONFH患者關(guān)節(jié)軟骨ADAMTS-7、TNF-α與磷酸化NF-κB均顯著上調(diào),ADAMTS-7的表達(dá)水平與軟骨TNF-α和磷酸化NF-κB P65的表達(dá)呈正相關(guān),ADAMTS-7可能通過TNF-α與磷酸化NF-κB P65參與ONFH的發(fā)病機(jī)制。
[Abstract]:Objective to investigate the correlation between ADAMTS-7 and the degeneration of articular cartilage and imaging changes in patients with osteonecrosis of the femoral head (ONFH). Methods 80 patients with femoral neck fracture treated in our hospital from March 2014 to June 2015 were included in the study. All the patients were divided into control group and ONFH group according to the results of the examination. There was no ONFH in the control group and no ONFH in the osteoarthritis group. According to the standard of ARCO staging, the X-ray examination of the patients in the control group had definite ONFH. The patients in ONFH group were further divided into two groups: group A and group A were divided into two groups: II~III stage X ray film showed that there was no obvious collapse of femoral head in group B and group B showed obvious collapse of femoral head in stage IV of ARCO stage. Mankin score was recorded. The expression of ADAMTS-7 TNF- 偽 and phosphorylated NF- 魏 B was analyzed by immunohistochemical staining. The mRNA level of ADAMTS-7 and the protein level of ADAMTS-7 and COMP were detected by RT-PCR and the protein levels of ADAMTS-7 and COMP were detected by Western Blot, and the difference of indexes was analyzed to explore the potential role of ADAMTS-7 in the pathogenesis of ONFH. There was no significant difference in sex and other basic information. The ADAMTS-7 and TNF- 偽 expression levels in ONFH group and ONFH B group were significantly higher than those in control group P0.05. ONFH group were significantly higher than that in control group P0.05. ONFH group. ADAMTS-7 in ONFH group was significantly higher than that in control group P0.05. ONFH group. The expression of ADAMTS-7 and TNF- 偽 in ONFH group were significantly higher than those in control group (P 0.05) and in ONFH group (P 0.05). The level of mRNA was significantly higher than that of control group (P 0.05). The results of Western Blot showed that the increase of ADAMTS-7 might be associated with the degradation of COMP. In addition, the expression of NF- 魏 B in patients with ONFH was significantly higher than that in control group. Conclusion the levels of ADAMTS-7 TNF- 偽 and phosphorylated NF- 魏 B in articular cartilage of ONFH patients were significantly higher than those in control group. The expression of ADAMTS-7 was positively correlated with the expression of TNF- 偽 and phosphorylated NF- 魏 B p65 in cartilage. ADAMTS-7 may be involved in the pathogenesis of ONFH through TNF- 偽 and phosphorylated NF- 魏 B p65.
【作者單位】： 西安交通大學(xué)醫(yī)學(xué)院;延安大學(xué)附屬醫(yī)院骨科;
【分類號(hào)】：R681.8

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本文編號(hào)：1580677