發(fā)布時(shí)間：2018-03-07 17:06

  本文選題：牛　切入點(diǎn)：胰島素受體基質(zhì)　出處：《河南農(nóng)業(yè)大學(xué)學(xué)報(bào)》2017年01期 　論文類型：期刊論文

【摘要】：以奶牛乳腺組織為材料,采用3’RACE技術(shù)克隆到奶牛IRS1基因3’UTR的全長,進(jìn)一步對(duì)測得的序列進(jìn)行了驗(yàn)證,同時(shí)利用Target Scan 7.0和Pic Tar軟件預(yù)測可能結(jié)合的microRNA,并對(duì)多個(gè)軟件預(yù)測結(jié)合的microRNA,進(jìn)行最小結(jié)合自由能分析。結(jié)果表明,成功克隆了1 327 bp的牛IRS1 3’UTR全長,與NCBI上的預(yù)測序列一致率達(dá)到99%;miR-128、miR-96、miR-27a為2個(gè)軟件共同預(yù)測到的結(jié)合microRNA;3個(gè)結(jié)合位點(diǎn)的最小自由能分別為-89.58、-87.91、-100.88 k J·mol-1,表明牛IRS1基因表達(dá)可能受到這3個(gè)microRNA的調(diào)控。
[Abstract]:The full-length IRS1 gene 3UTR of dairy cattle was cloned by using 3race technique from mammary tissue of dairy cattle, and the sequence was further verified. At the same time, Target Scan 7.0 and Pic Tar software were used to predict the possible binding microRNAs, and the minimum binding free energy of multiple microRNAs was analyzed. The results showed that 1 327 BP bovine IRS1 3 UTR was cloned successfully. The coincident rate of the predicted sequence with the predicted sequence on NCBI was 99%, and the minimum free energy of the three binding sites was -89.58 ~ 87.91 ~ 100.88 kJ 路mol ~ (-1), respectively, indicating that the expression of bovine IRS1 gene might be regulated by these three kinds of microRNA, and the minimum free energy of the three binding sites was -89.58% -87.91 ~ (-1) -100.88 kJ 路mol ~ (-1), respectively, which indicated that the expression of bovine IRS1 gene might be regulated by these three microRNA.
【作者單位】： 河南農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;河南省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所;
【基金】：國家自然科學(xué)基金項(xiàng)目(31501978) 河南省高等學(xué)校重點(diǎn)科研項(xiàng)目(16A230010)
【分類號(hào)】：S823
，

本文編號(hào)：1580182