本文選題：變異鏈球菌　切入點：乙�；D(zhuǎn)移酶　出處：《南方醫(yī)科大學學報》2017年06期 　論文類型：期刊論文

【摘要】：目的構(gòu)建變異鏈球菌(Streptococcus mutans,S.mutans)SMU.2055基因缺陷菌株,確定SMU.2055基因?qū).mutans形態(tài)及與致齲性相關(guān)的生物學特性的影響。方法利用同源重組的方法,構(gòu)建SMU.2055基因缺陷菌株。在掃描電子顯微鏡及透射電子顯微鏡下觀察野生菌株及基因缺陷菌株的形態(tài)結(jié)構(gòu)。連續(xù)監(jiān)測野生菌株及基因缺陷菌株的菌液A600值及p H值,比較其生長能力及產(chǎn)酸能力。對兩種菌株進行酸適應(yīng)后,分別進行酸沖擊,評價其耐酸能力。結(jié)果經(jīng)過聚合酶鏈反應(yīng)及測序鑒定,變異鏈球菌SMU.2055基因缺陷菌株構(gòu)建成功。掃描電子顯微鏡結(jié)果顯示,與野生菌株相比,基因缺陷菌株菌體形態(tài)發(fā)生改變,菌體間不規(guī)則物質(zhì)較少。透射電子顯微鏡下觀察,基因缺陷菌株細胞膜較模糊,細胞膜完整性被破壞。基因缺陷菌株的生長能力及耐酸能力較野生菌株均有所下降,而基因缺陷菌株與野生菌株間的產(chǎn)酸能力未見明顯差異。結(jié)論 SMU.2055基因與變異鏈球菌的形態(tài)、生長及耐酸能力密切相關(guān),而對其產(chǎn)酸能力不造成明顯影響。
[Abstract]:Objective to construct Streptococcus mutansanus S.mutansa SMU.2055 gene deficient strain and determine the effect of SMU.2055 gene on the morphology of S. mutans and the biological characteristics related to caries. Methods the homologous recombination method was used to determine the effect of SMU.2055 gene on the morphology of S. mutans and the biological characteristics related to caries. SMU.2055 gene deficient strains were constructed. The morphology and structure of wild and gene deficient strains were observed under scanning electron microscope and transmission electron microscope. A600 and pH values of wild and gene deficient strains were continuously monitored. The growth ability and acid production ability of the two strains were compared. After acid adaptation, the two strains were subjected to acid shock to evaluate their acid tolerance. The results were confirmed by polymerase chain reaction (PCR) and sequencing. Streptococcus mutans SMU.2055 gene deficient strain was successfully constructed. The results of scanning electron microscope showed that the morphology of the mutant strain changed compared with that of wild strain, and there were fewer irregular substances between them. The cell membrane of the gene deficient strain was blurred and the integrity of the cell membrane was destroyed. The growth ability and acid tolerance ability of the gene deficient strain were lower than those of the wild strain. Conclusion the SMU.2055 gene is closely related to the morphology, growth and acid-tolerance of Streptococcus mutans, but has no significant effect on the acid-producing ability of Streptococcus mutans.
【作者單位】： 南方醫(yī)科大學南方醫(yī)院口腔科;南方醫(yī)科大學口腔醫(yī)學院;深圳市龍華新區(qū)中心醫(yī)院口腔科;南方醫(yī)科大學附屬廣東省口腔醫(yī)院;
【基金】：南方醫(yī)科大學南方醫(yī)院院長基金(2013B003) 深圳市科技計劃項目(JCYJ20160428142231354)
【分類號】：R781.1

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本文編號：1574373