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  本文選題：蛹蟲草　切入點：蟬棒束孢　出處：《菌物學(xué)報》2017年02期 　論文類型：期刊論文

【摘要】：組氨酸激酶是真菌感受外界刺激的重要組成部分,它使真菌能適應(yīng)和應(yīng)答環(huán)境的改變。通過RT PCR克隆蟬棒束孢Isaria cicadae組氨酸激酶基因Ic HK的ORF序列,對Ic HK基因序列分析表明其翻譯產(chǎn)物Ic HK中存在著4個HAMP功能域、1個His KA功能域、1個HATPase功能域和1個REC功能域。采用實時熒光定量PCR方法研究了3種殺菌劑咯菌腈(Fludioxonil)、異菌脲(Iprodione)和克菌丹(Captan)對蛹蟲草Cordyceps militaris組氨酸激酶Cm HK和蟬棒束孢組氨酸激酶Ic HK表達的影響。結(jié)果表明,Cm HK基因的表達對這3種殺菌劑的影響不敏感,這3種殺菌劑不能導(dǎo)致Cm HK基因的相對轉(zhuǎn)錄量明顯增加。Ic HK基因的表達對這3種殺菌劑的影響都敏感,特別是對咯菌腈和克菌丹更為敏感,這3種殺菌劑可導(dǎo)致Ic HK基因的相對轉(zhuǎn)錄量的增加。研究結(jié)果表明不同真菌應(yīng)對相同脅迫環(huán)境時,同源基因的表達特征可存在差異。
[Abstract]:Histidine kinase (histidine kinase) is an important component of fungal response to external stimuli, which enables fungi to adapt to and respond to environmental changes. The ORF sequence of Isaria cicadae histidine kinase gene IcHK was cloned by RT PCR. Sequence analysis of IcHK gene showed that there were 4 HAMP functional domains, 1 His Ka functional domain, 1 HATPase functional domain and 1 REC functional domain in its translation product IcHK. Three fungicides were studied by real-time fluorescence quantitative PCR method. The effects of Fludioxonil (Iprodione) and Captan) on the expression of Cordyceps militaris histidine kinase (km HK) and histidine kinase (IC HK) in Cordyceps militaris were studied. The results showed that the expression of CmHK gene was not sensitive to these three fungicides. These three fungicides could not cause the relative transcription of Cm HK gene to increase significantly. The expression of IC HK gene was sensitive to these three fungicides, especially to pyrrolionitrile and kebendan. These three fungicides could increase the relative transcription of IcHK gene. The results showed that the expression characteristics of the homologous genes could be different when different fungi responded to the same stress environment.
【作者單位】： 江西師范大學(xué)生命科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(31060004,31260010)~~
【分類號】：S567.3

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