本文選題：TaTGW6　切入點(diǎn)：dCAPS　出處：《中國(guó)農(nóng)業(yè)科學(xué)院》2016年博士論文　論文類(lèi)型：學(xué)位論文

【摘要】：TGW6編碼一個(gè)新型吲哚乙酸葡萄糖水解酶,對(duì)水稻千粒重和產(chǎn)量都有顯著影響。通過(guò)同源克隆分離了來(lái)自于小麥同源染色體群2L、3L和7S的9個(gè)TGW6同源基因。它們都沒(méi)有內(nèi)含子。為了檢測(cè)這些基因的多態(tài)性,兩組千粒重顯著不同的8個(gè)小麥品系用作分子標(biāo)記開(kāi)發(fā)的初始材料,其中高千粒重品種包括豆麥、PH85-1-1、鄂86642和臨旱6114；低千粒重品種包括洋小麥、豫麥21、山東955159和陜898-33。通過(guò)克隆測(cè)序獲得了這8個(gè)品系的所用9個(gè)TGW同源基因,其中只有TaTGW6-A1l有三個(gè)單核苷酸多態(tài)性(SNP)位點(diǎn)顯著與千粒重關(guān)聯(lián),并且這三個(gè)SNP形成兩個(gè)單倍型：TaTGW6-A1a和TaTGW6-A1b。根據(jù)這兩個(gè)單倍型,開(kāi)發(fā)了一個(gè)衍生酶切擴(kuò)增多態(tài)性序列(dCAPS)分子標(biāo)記TaTGW6-A1-CAPS。利用中國(guó)春/周8425B的近等基因系(RIL)作圖群體并結(jié)合小麥90K iSelect SNP芯片進(jìn)行連鎖分析,TaTGW6-A1-CAPS被進(jìn)一步定位在小麥3A染色體長(zhǎng)臂的SNP標(biāo)記BobWhite_c47304_56和GENE_3365_61之間,并且TaTGW6-Al-CAPS和BobWhite_c47304_56之間檢測(cè)到了一個(gè)控制產(chǎn)量的QTL。在四個(gè)環(huán)境下這個(gè)QTL可以解釋平均17.4%的表型變異。利用不同小麥生態(tài)區(qū)的242個(gè)小麥品系進(jìn)行關(guān)聯(lián)分析,結(jié)果顯示TaTGW6-A1和千粒重顯著相關(guān)。進(jìn)一步分析表明約80%小麥品種具有高千粒重的單倍型TaTGW6-A1a,說(shuō)明在育種實(shí)踐中對(duì)TaTGW6的高千粒重單倍型已經(jīng)進(jìn)行了正選擇�？傊�,小麥TGW6同源基因的分離和相應(yīng)分子標(biāo)記的開(kāi)發(fā)為小麥高產(chǎn)育種提供了很有利用價(jià)值的基因信息和工具。
[Abstract]:TGW6 encodes a novel indoleacetic acid glucose hydrolase. Nine TGW6 homologous genes from wheat homologous chromosome group 2Ln3L and 7s were isolated by homologous cloning. None of them had introns. In order to detect the polymorphism of these genes, Eight wheat lines with significantly different 1000-grain weights were used as the initial materials for molecular marker development, among which the high 1000-grain weight varieties included Ph85-1-1, E 86642 and Linhan 6114, and the low 1000-grain weight varieties included Yang wheat. Yumai 21, Shandong 955159 and Shan 898-33.Nine TGW homologous genes were obtained by cloning and sequencing. Among them, only three single nucleotide polymorphisms (SNPs) were found in Yumai 21, Shandong 955159 and Shan898-33. among them, only three single nucleotide polymorphisms (SNPs) were significantly associated with 1000-grain weight. And the three SNP forms two haplotypes: TaTGW6-A1a and TaTGW6-A1b. according to these haplotypes, A molecular marker TaTGW6-A1-CAPS. using Chinese Spring / Zhou8425B near isogenic line rill was developed and linked to wheat 90K iSelect SNP microarray. TaTGW6-A1-CAPS was further located in wheat 3A staining. The SNP of the long arm marked between BobWhite_c47304_56 and GENE_3365_61. And a QTL was detected between TaTGW6-Al-CAPS and BobWhite_c47304_56, which could explain an average of 17.4% phenotypic variations in four environments, using 242 wheat lines in different wheat ecological regions for correlation analysis. The results showed that there was a significant correlation between TaTGW6-A1 and 1000-grain weight. Further analysis showed that about 80% wheat varieties had high 1000-grain weight haplotype TaTGW6-A1a, which indicated that the high 1000-grain weight haplotype of TaTGW6 had been selected in breeding practice. The isolation of wheat TGW6 homologous genes and the development of corresponding molecular markers provide valuable genetic information and tools for wheat breeding with high yield.
【學(xué)位授予單位】：中國(guó)農(nóng)業(yè)科學(xué)院
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：S512.1
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本文編號(hào)：1571949