本文選題：棉花　切入點：SNC　出處：《分子植物育種》2017年01期 　論文類型：期刊論文

【摘要】：以陸地棉(Gossypium hirsutum)TM-1為材料,利用同源克隆技術(shù)從棉花中分離出SNC2基因,以actin基因為內(nèi)參,采用半定量RT-PCR分析該基因在棉花組織中的表達,并采用實時定量RT-PCR分析不同棉花品種經(jīng)大麗輪枝菌侵染后,SNC2基因在不同時段、不同組織的表達水平。研究分析發(fā)現(xiàn)該基因CDS序列長度為1 308 bp,編碼435個氨基酸,蛋白的等電點(p I)為6.98,理論分子量為46.93 k D,命名為SNC2,Gen Bank登錄號為AOO35322.1,SNC2基因編碼多肽鏈中包含信號肽和跨膜結(jié)構(gòu)域,且亞細胞定位于質(zhì)膜,編碼受體類蛋白。組織表達分析顯示SNC2基因在根、側(cè)根、莖、葉和花中均有表達,莖中的表達量較高,而在苞片組織中基本不表達。該基因在接菌后的感病品種軍棉1號的根和耐病品種中棉49號的莖中呈現(xiàn)不斷上升的趨勢,而在兩個棉花品種的其他組織中呈現(xiàn)出"升-降-升"的趨勢,且在不同抗性的棉花品種中的表達存在差異,暗示SNC2基因可能在不同抗性品種抵御生物脅迫過程中扮演重要角色,研究結(jié)果為進一步研究SNC2基因的功能及應用該基因提供幫助。
[Abstract]:Using Gossypium hirsutum)TM-1 as material, SNC2 gene was isolated from cotton by homologous cloning technique. The expression of SNC2 gene in cotton tissue was analyzed by semi-quantitative RT-PCR using actin as internal reference. Real-time quantitative RT-PCR was used to analyze the expression level of SNC2 gene in different tissues of different cotton varieties infected by Verticillium dahliensis. The length of CDS sequence of this gene was 1 308 BP, encoding 435 amino acids. The isoelectric point I) of the protein is 6.98, and the theoretical molecular weight is 46.93 KD. The accession number of SNC2Gen Bank is AOO35322.1 and SNC2 gene encodes a signal peptide and a transmembrane domain, and the subcells are located in the plasma membrane. The expression of SNC2 gene was found in roots, lateral roots, stems, leaves and flowers. But it was not expressed in bracts. The gene showed an increasing trend in the roots of susceptible variety Junmian 1 and the stem of susceptible variety Zhongmian49 after inoculation. However, in other tissues of the two cotton varieties, there was a tendency of "up-down-rise-rise-rise", and there were differences in expression in different resistant cotton varieties. It is suggested that SNC2 gene may play an important role in the process of resistance to biological stress in different resistant varieties, and the results may be helpful to further study the function of SNC2 gene and its application.
【作者單位】： 新疆師范大學生命科學學院;新疆農(nóng)業(yè)科學院核技術(shù)生物技術(shù)研究所新疆農(nóng)作物生物技術(shù)重點實驗室新疆農(nóng)業(yè)科學院棉花分子機理與分子育種實驗室;
【基金】：新疆維吾爾自治區(qū)自治區(qū)自然基金項目(2013211A037)資助
【分類號】：S435.62;Q943.2
，

本文編號：1571537