本文選題：BjMnSOD　切入點(diǎn)：芥菜　出處：《植物生理學(xué)報(bào)》2017年04期 　論文類(lèi)型：期刊論文

【摘要】：本文通過(guò)RACE的方法克隆獲得了芥菜超氧化物歧化酶基因序列,命名為Bj Mn SOD1。該基因全長(zhǎng)1 003 bp,包括696bp的開(kāi)放閱讀框,編碼231個(gè)氨基酸。預(yù)測(cè)蛋白分子量25.4 k Da,等電點(diǎn)為8.38。氨基酸序列比對(duì)分析表明,Bj Mn SOD1同甘藍(lán)、油菜和白菜的同源性分別為96%、97%和97%。同時(shí)具有典型的錳超氧化物歧化酶結(jié)構(gòu)特征。系統(tǒng)進(jìn)化分析表明Bj Mn SOD1和十字花科植物在進(jìn)化上親緣關(guān)系較近。半定量和熒光定量PCR結(jié)果表明,Bj Mn SOD1可以響應(yīng)各種脅迫和激素處理。其中鹽處理后的動(dòng)態(tài)分析發(fā)現(xiàn),Bj Mn SOD1轉(zhuǎn)錄水平的變化和總SOD酶活性變化趨勢(shì)基本一致。這些結(jié)果表明,Bj Mn SOD1屬于超氧化物歧化酶基因家族成員中的Mn SOD,并可能在芥菜響應(yīng)逆境脅迫的過(guò)程中起了重要作用。
[Abstract]:In this paper, the superoxide dismutase gene of mustard was cloned by RACE. The gene was named Bj mn SOD 1. The total length of the gene was 1 003 BP, including an open reading frame of 696 BP. The predicted molecular weight of the protein was 25.4 kDa and the isoelectric point was 8.38. The amino acid sequence alignment analysis showed that Bj mn SOD1 was the same as cabbage. The homology of Brassica napus and Brassica campestris were 9697% and 977.At the same time, they had typical structural characteristics of manganese superoxide dismutase. Phylogenetic analysis showed that Bj mn SOD1 and cruciferous plants had close phylogenetic relationship. The results of quantitative PCR showed that Bj mn SOD1 could respond to various stress and hormone treatment, and the dynamic analysis after salt treatment showed that the change of SOD1 transcription level of Bj mn was basically consistent with the change trend of total SOD enzyme activity. These results indicated that the change trend of Bj mn was similar to that of Bj mn. SOD1 is a member of superoxide dismutase gene family mn SOD and may play an important role in the response of mustard to stress.
【作者單位】： 河南農(nóng)業(yè)大學(xué)園藝學(xué)院;鄭州市蔬菜研究所十字花科研究課題組;
【基金】：國(guó)家自然科學(xué)基金(31301768) 河南省高等學(xué)校重點(diǎn)科研項(xiàng)目(16A210031)~~
【分類(lèi)號(hào)】：Q943.2;S637
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本文編號(hào)：1570112