本文選題：大葉落地生根　切入點：類糖原合成酶激酶　出處：《廣西植物》2017年08期 　論文類型：期刊論文

【摘要】：類糖原合成酶激酶(SKs)屬于絲氨酸/蘇氨酸類蛋白激酶,在植物器官發(fā)育、激素信號傳導過程中十分重要,并參與生物脅迫、非生物脅迫的應答過程。大葉落地生根中的胎生苗發(fā)育過程,同時具備胚胎發(fā)生和器官發(fā)生的特征,是研究無性生殖的理想模型。為了更好地理解大葉落地生根中胎生苗發(fā)育的分子機制,該研究利用RACE-PCR技術,從大葉落地生根中克隆了1個新的基因KdSK。該基因具有423個氨基酸殘基,分子量為47.79 kD,等電點為8.37,其開放閱讀框長為1 272 bp。其蛋白與黃瓜的同源性最高,屬于植物類GSK3/shaggy蛋白激酶家族的第Ⅳ類,與苜蓿(MSK4)蛋白在進化關系上最近,且與擬南芥(AtSK4-1、AtKSK4-2)聚為一枝。保守域結構分析表明,KdSK蛋白具有明顯的蛋白激酶的結構域,包括蛋白激酶的ATP結構域和絲氨酸/蘇氨酸蛋白激酶活化結構域。實時熒光定量PCR分析表明,該蛋白基因在大葉落地生根的根中表達量最高,且受滲透脅迫(甘露醇)的誘導上調表達。該研究首次從大葉落地生根中克隆出KdSK基因,該研究結果為進一步研究該基因的功能打下了基礎。
[Abstract]:Glycogen synthase kinase (SKs) is a serine / threonine protein kinase, which plays an important role in plant organ development and hormone signal transduction, and is involved in biological stress. Response to abiotic stress. The development of plantlets in the rooting of large leaves, with the characteristics of embryogenesis and organogenesis. It is an ideal model for studying asexual reproduction. In order to better understand the molecular mechanism of embryogenic seedling development in the rooting of large leaf, RACE-PCR technique was used in this study. A new gene KdSK was cloned from the rooting of Lymphoma macrophylla. The gene has 423 amino acid residues with molecular weight of 47.79 KD, isoelectric point of 8.37, and its open reading frame length is 1 272 BP. The protein has the highest homology with cucumber. It belongs to the fourth class of plant GSK3/shaggy protein kinases family, and has the closest evolutionary relationship with the alfalfa protein MSK4, and is clustered into one branch with Arabidopsis thaliana AtSK4-1 (AtKSK4-2). The conserved domain analysis shows that the KDSK protein has a distinct protein kinase domain. The ATP domain including protein kinase and serine / threonine protein kinase activated domain. Real-time quantitative PCR analysis showed that the protein gene was the most expressed in rooting roots. The expression of KdSK gene was firstly cloned from the rooting of Lymphoma macrophylla under osmotic stress (mannitol). The results laid a foundation for further study on the function of the gene.
【作者單位】： 深圳市日f園林綠化有限公司;暨南大學深圳旅游學院;北京林業(yè)大學林學院草坪研究所;華南農業(yè)大學林學與風景園林學院廣東省草業(yè)工程技術研究中心;
【基金】：深圳市科技計劃項目(CXZZ20140418110342522) 北京林業(yè)大學與廈門日懋城建園林建設股份有限公司產學研合作項目~~
【分類號】：S682.33

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