本文選題：水牛　切入點(diǎn)：甲狀旁腺素　出處：《中國(guó)畜牧獸醫(yī)》2017年09期 　論文類(lèi)型：期刊論文

【摘要】：本研究參考黃牛甲狀旁腺素(parathyroid hormone,PTH)基因序列設(shè)計(jì)引物,運(yùn)用PCR擴(kuò)增技術(shù)對(duì)水牛PTH基因進(jìn)行克隆,并用生物信息學(xué)方法對(duì)序列進(jìn)行分析。結(jié)果顯示,成功克隆了水牛PTH基因完整編碼區(qū)序列(CDS)348bp,可編碼115個(gè)氨基酸。該基因編碼區(qū)序列與黃牛、豬、馬、山羊、綿羊和駱駝的同源性分別為99%、93%、91%、97%、96%和92%,表明PTH基因在不同物種間高度保守。系統(tǒng)進(jìn)化樹(shù)分析表明,水牛與黃牛PTH基因親緣關(guān)系最近。此外,在水牛PTH基因CDS上共發(fā)現(xiàn)了3個(gè)堿基突變,其中兩個(gè)屬于同義突變,一個(gè)屬于錯(cuò)義突變。氨基酸序列分析表明,該蛋白屬于堿性、親水、穩(wěn)定型蛋白質(zhì),其分子式為C_(570)H_(941)N_(167)O_(164)S_8,分子質(zhì)量為13.01ku。二級(jí)結(jié)構(gòu)分析顯示,水牛PTH蛋白以α-螺旋和無(wú)規(guī)則卷曲為主,與三級(jí)結(jié)構(gòu)預(yù)測(cè)結(jié)果相一致。亞細(xì)胞定位分析顯示,水牛PTH蛋白分布在細(xì)胞質(zhì)(26.1%)、線(xiàn)粒體(21.7%)、細(xì)胞外(包括細(xì)胞壁)(17.4%)、內(nèi)質(zhì)網(wǎng)(13.0%)、細(xì)胞核(8.7%)、高爾基體(4.3%)和其他部位(8.7%),推測(cè)PTH蛋白可能在運(yùn)輸、結(jié)合及細(xì)胞被膜等方面發(fā)揮信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄調(diào)控作用。
[Abstract]:In this study, we designed primers for the sequence of parathyroid hormoney (PTH) gene of bovine parathyroid hormone, cloned the PTH gene of buffalo by PCR amplification technique, and analyzed the sequence by bioinformatics. The complete coding region of buffalo PTH gene was cloned successfully, which can encode 115 amino acids. The sequence of the complete coding region of buffalo PTH gene is similar to that of cattle, pig, horse, goat, yellow cattle, pig, horse and goat. The homology of sheep and camel were 9993and 9197%, respectively, indicating that the PTH gene was highly conserved among different species. Phylogenetic tree analysis showed that the PTH gene of buffalo and yellow cattle had the closest relationship. Three base mutations were found in buffalo PTH gene CDS, two of which were synonymous mutations and one was missense mutation. The amino acid sequence analysis showed that the protein belonged to alkaline, hydrophilic and stable proteins. The molecular formula of this protein is: Che 570H / L / N _ S _ (167O) / S _ (8) and molecular mass 13.01ku.The secondary structure analysis shows that the PTH protein of buffalo is mainly 偽 -helix and irregular curl, which is consistent with the prediction result of tertiary structure. Subcellular localization analysis shows that the molecular weight is 13.01ku.Subcellular localization analysis shows that the protein is mainly 偽 -helix and irregular curl, and is consistent with the predicted results of tertiary structure. The PTH protein of buffalo is distributed in cytoplasm 26.1%, mitochondria 21.7m, extracellular (including cell wall 17.4, endoplasmic reticulum 13.0, nucleus 8.7, Golgi 4.33) and other parts, suggesting that PTH protein may be transported. Binding and cell membrane play a role in signal transduction and transcriptional regulation.
【作者單位】： 中國(guó)農(nóng)業(yè)科學(xué)院廣西水牛研究所農(nóng)業(yè)部(廣西)水牛遺傳繁育重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31660649) 廣西科技重大專(zhuān)項(xiàng)(桂科AA16450002) 廣西科技公關(guān)國(guó)合項(xiàng)目(桂科合1504001-3) 水�；�160207 水�；�1705005
【分類(lèi)號(hào)】：Q811.4;S823.83

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊武,于小鵬;甲狀旁腺素對(duì)血管及其它平滑肌的調(diào)節(jié)作用[J];國(guó)外醫(yī)學(xué).內(nèi)分泌學(xué)分冊(cè);1984年03期

2 王振綱,曾湘平;為什么最近重視甲狀旁腺素的研究?[J];中國(guó)藥理學(xué)通報(bào);1985年01期

3 Bilezikian JP ,馬玉銘;病人和健康人的甲狀旁腺素相關(guān)肽[J];國(guó)外醫(yī)學(xué).內(nèi)分泌學(xué)分冊(cè);1991年04期

4 尹積榮;楊凱;白玉興;王邦康;;甲狀旁腺素的生物信息學(xué)分析[J];口腔醫(yī)學(xué)研究;2011年07期

5 劉長(zhǎng)劍;張?jiān)?;甲狀旁腺素促進(jìn)成骨的研究[J];長(zhǎng)春中醫(yī)藥大學(xué)學(xué)報(bào);2010年03期

6 白秀英,俞文華,周愛(ài)儒,杜國(guó)光;甲狀旁腺素對(duì)成骨樣細(xì)胞增殖的調(diào)節(jié)作用[J];中國(guó)生物化學(xué)與分子生物學(xué)報(bào);1999年01期

7 周賀鉞,修朝陽(yáng),陳常慶;甲狀旁腺素1-34肽的固相合成[J];中國(guó)生化藥物雜志;2002年03期

8 鄭燕倩,李慈珍,張穎,劉遠(yuǎn)謀;甲狀旁腺素增加心率的機(jī)制研究[J];上海第二醫(yī)科大學(xué)學(xué)報(bào);2005年06期

9 趙睿珊,王輔才,朱文萍,胡曉東;甲狀旁腺高血壓因子和甲狀旁腺素對(duì)細(xì)胞鈣通道的不同作用[J];生物化學(xué)雜志;1997年03期

10 劉長(zhǎng)劍;王李;羅宗鍵;;低能體外沖擊波和低劑量間歇甲狀旁腺素干預(yù)成骨細(xì)胞的增殖和分化（英文）[J];中國(guó)組織工程研究;2014年11期

相關(guān)會(huì)議論文 前1條

1 朱波;;甲狀旁腺素受體1基因沉默INS-1細(xì)胞模型的構(gòu)建及鑒定[A];2010中國(guó)醫(yī)師協(xié)會(huì)內(nèi)分泌代謝科醫(yī)師分會(huì)年會(huì)論文匯編[C];2010年

相關(guān)博士學(xué)位論文 前1條

1 劉雅紅;甲狀旁腺素相關(guān)肽核定位序列和C末端在調(diào)節(jié)中樞神經(jīng)系統(tǒng)發(fā)育中的作用及機(jī)制研究[D];南京醫(yī)科大學(xué);2013年

，

本文編號(hào)：1565713