發(fā)布時間：2018-03-04 09:15

  本文選題：水稻　切入點：溶磷菌　出處：《華南農業(yè)大學學報》2017年02期 　論文類型：期刊論文

【摘要】：【目的】克隆葡萄糖酸脫氫酶(GADH)小亞基基因ga2dh并進行鑒定�！痉椒ā垦芯克靖H細菌Serratia sp.NDW3溶磷過程中菌株溶磷量、GADH活性與ga2dh基因表達量的變化,對ga2dh基因進行克隆和生物信息學分析,并檢測ga2dh基因在大腸埃希菌BL21中的表達�！窘Y果】Serratia sp.NDW3溶磷過程中ga2dh基因的相對表達量在12 h最大,GADH活性在24 h達到最大值,NDW3溶磷量在36 h后趨于穩(wěn)定。從Serratia sp.NDW3菌株中克隆獲得了781 bp的ga2dh基因序列,生物信息學分析發(fā)現(xiàn)該序列與Serratia sp.SCBI菌株的基因相似性為99.62%,編碼的蛋白屬于葡萄糖酸脫氫酶亞基3超家族,主要由3個α-螺旋構成,且氨基酸序列中包含有位于胞內、胞外和跨膜的區(qū)域。ga2dh基因在大腸埃希菌BL21體內表達,能夠使得菌體GADH的活性顯著增加�！窘Y論】Serratia sp.NDW3菌株溶磷的主要機制依賴于直接氧化途徑,ga2dh基因編碼的小亞基不僅對GADH活性起重要作用,也是介導GADH跨膜結構的重要組成部分。
[Abstract]:[objective] to clone and identify the small subunit gene ga2dh of gluconate dehydrogenase (GADH). [methods] to study the changes of the activity of GADH and the expression of ga2dh gene during phosphorus dissolution of Serratia sp.NDW3 in rice rhizosphere bacteria. Cloning and bioinformatics analysis of ga2dh gene, The expression of ga2dh gene in Escherichia coli BL21 was detected. [results] the relative expression of ga2dh gene reached the maximum at 24 h after 12 h of Serratia sp.NDW3 phosphorolysis. After 36 h, it tended to be stable from Serratia sp.NDW3 strain. The ga2dh gene sequence of 781bp was obtained. Bioinformatics analysis showed that the gene similarity between this sequence and Serratia sp.SCBI strain was 99.62, and the encoded protein belonged to the gluconate dehydrogenase subunit 3 superfamily, which was mainly composed of three 偽 -helix, and the amino acid sequence contained in the intracellular. The extracellular and transmembrane region. Ga2dh gene was expressed in BL21 of Escherichia coli. [conclusion] the main mechanism of phosphorus solubilization of Serratia sp.NDW3 strain depends on the direct oxidation pathway. The small subunit encoded by ga2dh gene not only plays an important role in the activity of GADH, but also plays an important role in mediating the transmembrane structure of GADH.
【作者單位】： 吉林農業(yè)大學生命科學學院;吉林農業(yè)大學農學院;
【分類號】：S565.101

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