本文關(guān)鍵詞： 大豆 木質(zhì)素 Glymag 植物防御　出處：《基因組學(xué)與應(yīng)用生物學(xué)》2016年10期 　論文類型：期刊論文

【摘要】：擬南芥At PRXs基因家族屬于過氧化物酶類,在植物木質(zhì)素形成過程中發(fā)揮了重要的作用。本研究對ATPRX在大豆中的同源基因Glyma06g28890進(jìn)行基因結(jié)構(gòu)功能預(yù)測和表達(dá)分析,發(fā)現(xiàn)了Glyma06g28890和At PRXs相同,具有一個保守的Plant peroxidase結(jié)構(gòu)域,并且與ATPRX25具有最高的相似度;通過啟動子順式作用元件統(tǒng)計,發(fā)現(xiàn)在基因啟動子序列中含有厭氧誘導(dǎo)調(diào)節(jié)、激素響應(yīng)、分生組織表達(dá)以及植物防御相關(guān)的元件。Glyma06g28890在大豆的組織特異性表達(dá)分析結(jié)果表明,它在植物內(nèi)木質(zhì)素廣泛分布的組織內(nèi),如根和莖內(nèi)都有較高水平的表達(dá)。
[Abstract]:The at PRXs gene family of Arabidopsis thaliana belongs to peroxidase and plays an important role in the formation of plant lignin. In this study, the gene structure and function of homologous gene Glyma06g28890 of ATPRX in soybean were predicted and expressed. It was found that Glyma06g28890 and at PRXs share the same Plant peroxidase domain and have the highest similarity with ATPRX25. Through the statistics of cis-acting elements of promoter, it is found that the promoter sequence contains anaerobic-induced regulation and hormone response. The results of tissue specific expression analysis of Glyma06g28890 in soybean showed that Glyma06g28890 had a high level of expression in the tissue where lignin was widely distributed in plants, such as roots and stems.
【作者單位】： 東北農(nóng)業(yè)大學(xué)大豆研究所大豆生物學(xué)教育部重點實驗室/農(nóng)業(yè)部東北大豆生物學(xué)與遺傳育種重點實驗室;
【基金】：抗逆轉(zhuǎn)基因大豆新品種培育專項(2014ZX08004-002) 國家自然科學(xué)基金項目(31201228)共同資助
【分類號】：Q943.2;S565.1
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本文編號：1552217