發(fā)布時(shí)間：2018-02-28 18:50

  本文關(guān)鍵詞： 鎘 三七 關(guān)鍵酶 基因表達(dá) 藥效成分　出處：《中草藥》2016年24期 　論文類型：期刊論文

【摘要】：目的研究外源性鎘(Cd)對(duì)三七主要藥效成分合成中關(guān)鍵酶2,3-氧化鯊烯被達(dá)瑪烯二醇合成酶(DS)和細(xì)胞色素P450單加氧酶(P450)基因表達(dá)的影響。方法設(shè)置9個(gè)Cd的質(zhì)量分?jǐn)?shù)梯度(0.0、0.1、0.3、0.6、1.0、3.0、6.0、10.0、30.0 mg/kg)分別對(duì)三七進(jìn)行脅迫實(shí)驗(yàn),兩年后采集成熟三七,通過(guò)q RT-PCR檢測(cè)手段對(duì)其根部DS及P450基因進(jìn)行表達(dá)量測(cè)定,并以GAPDH基因作為內(nèi)參基因進(jìn)行DS及P450基因相對(duì)表達(dá)量的計(jì)算,通過(guò)相關(guān)性分析計(jì)算DS和P450基因表達(dá)與三七主要藥效成分三七皂苷R1、人參皂苷Rb1、人參皂苷Rg1以及總皂苷的相關(guān)性。結(jié)果 Cd在低質(zhì)量分?jǐn)?shù)下能促進(jìn)DS的表達(dá),高質(zhì)量分?jǐn)?shù)下抑制DS的表達(dá);隨著Cd質(zhì)量分?jǐn)?shù)的增加,對(duì)P450表達(dá)的抑制作用更加明顯;而DS和P450基因表達(dá)與三七主要藥效成分三七皂苷R1、人參皂苷Rb1、人參皂苷Rg1以及總皂苷無(wú)顯著相關(guān)性(P0.5)。結(jié)論重金屬Cd對(duì)三七藥效成分中關(guān)鍵酶DS和P450基因表達(dá)影響不同,對(duì)同一種酶,Cd質(zhì)量分?jǐn)?shù)不同其影響也不同。但主要藥效成分并未因此受明顯影響。
[Abstract]:Objective to investigate the effects of cadmium (Cd) on the oxidation of shark key enzyme of 2,3- synthesis by the 37 major components in graphene dammarenediol synthase (DS) and cytochrome P450 monooxygenase (P450) gene expression of the mass fraction gradient method set 9 Cd (0.0,0.1,0.3,0.6,1.0,3.0,6.0,10.0,30.0 mg/kg) respectively for 37 stress experiment 37, two years after the acquisition of the root mature, DS and P450 gene expression was detected by means of Q, RT-PCR, and GAPDH gene as a control gene DS and P450 gene of the relative amount of calculation, through correlation analysis, calculation of DS and P450 gene expression and 37 of the 37 major components of ginsenoside R1, ginsenoside Rb1 correlation, ginsenoside Rg1 and total saponins. The results of Cd in low concentration could promote the expression of DS and high concentration inhibited the expression of DS; with the increase of mass fraction of Cd, The more obvious inhibitory effect on P450 expression; DS and P450 gene expression and 37 of the 37 major components of ginsenoside R1, ginsenoside Rb1, ginsenoside Rg1 and no significant correlation between total saponins (P0.5). Conclusion Cd on the 37 components of heavy metals in key enzymes DS and P450 gene expression in different effects on the same enzyme the mass fraction of Cd, its influence is also different. But different major components did not significantly affected.

【作者單位】： 北京師范大學(xué)中藥資源保護(hù)與利用北京市重點(diǎn)實(shí)驗(yàn)室;寧夏醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院醫(yī)用化學(xué)系;北京師范大學(xué)資源藥物教育部工程研究中心;
【分類號(hào)】：S567.236
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本文編號(hào)：1548520