本文關鍵詞： 高血壓 當歸揮發(fā)油 miR- 血管緊張素Ⅱ型受體拮抗劑　出處：《中國全科醫(yī)學》2017年09期 　論文類型：期刊論文

【摘要】：目的探究當歸揮發(fā)油對自發(fā)性高血壓大鼠(SHR)miR-155及其靶基因的影響,并從腎素-血管緊張素-醛固酮系統(tǒng)(RAAS)探討當歸揮發(fā)油對SHR血壓的改善作用及其機制。方法 2015年10月—2016年4月,將8周齡48只SPF級雄性SHR隨機分為模型組、當歸揮發(fā)油低劑量組、當歸揮發(fā)油中劑量組、當歸揮發(fā)油高劑量組、藁本內酯組、纈沙坦組,各8只;另選取8只SPF級正常血壓雄性Wistar大鼠作為正常組。正常組、模型組不給予任何藥物,當歸揮發(fā)油低劑量組、當歸揮發(fā)油中劑量組、當歸揮發(fā)油高劑量組、藁本內酯組、纈沙坦組大鼠分別灌胃給予當歸揮發(fā)油乳劑12.6 mg·kg~(-1)·d~(-1)、37.0 mg·kg~(-1)·d~(-1)、100.0 mg·kg~(-1)·d~(-1),藁本內酯溶液0.8 mg·kg~(-1)·d~(-1),纈沙坦溶液51.4 mg·kg~(-1)·d~(-1),連續(xù)給藥4周,給藥期間各組大鼠標準飲食、自由飲水。檢測給藥前及給藥第1、2、3、4周各組大鼠收縮壓變化。灌胃4周后處死大鼠取心臟,HE染色觀察心肌組織病理學的變化,采用Western blotting法檢測血管緊張素Ⅱ1型受體(AT1R)、細胞外調節(jié)蛋白激酶1/2(ERK1/2)、磷酸化ERK1/2(p-ERK1/2)表達水平。采用RT-qPCR法檢測miR-155表達水平。結果不同處理情況、時間在大鼠收縮壓中存在交互作用(P0.05);不同處理情況、時間在大鼠收縮壓中主效應顯著(P0.05)。給藥前及給藥第1、2、3周,模型組、當歸揮發(fā)油低劑量組、當歸揮發(fā)油中劑量組、當歸揮發(fā)油高劑量組、藁本內酯組、纈沙坦組大鼠收縮壓均高于正常組(P0.05);給藥第4周模型組大鼠收縮壓高于正常組(P0.05);給藥前當歸揮發(fā)油低劑量組、當歸揮發(fā)油中劑量組、當歸揮發(fā)油高劑量組、藁本內酯組、纈沙坦組大鼠收縮壓均高于模型組,給藥第1、2、3、4周當歸揮發(fā)油低劑量組、當歸揮發(fā)油中劑量組、當歸揮發(fā)油高劑量組、藁本內酯組、纈沙坦組大鼠收縮壓均低于模型組(P0.05)。HE染色顯示,模型組大鼠心肌組織紅細胞多而擠,心肌橫紋不清,提示心肌充血,右心功能受損;當歸揮發(fā)油低、中、高劑量組大鼠心肌組織出現(xiàn)橫紋且清晰。當歸揮發(fā)油低劑量組、當歸揮發(fā)油中劑量組、當歸揮發(fā)油高劑量組、藁本內酯組、纈沙坦組大鼠AT1R、ERK1/2、p-ERK1/2表達水平均低于模型組(P0.05)。7組大鼠miR-155表達水平比較,差異無統(tǒng)計學意義(P0.05)。結論當歸揮發(fā)油能夠明顯抑制AT1R基因的表達,通過調節(jié)ERK1/2信號通路從而達到降壓的功能;當歸揮發(fā)油不通過miR-155在心肌組織發(fā)揮作用。
[Abstract]:Objective to investigate the effect of volatile oil of Angelica sinensis on SHR-miR-155 and its target gene in spontaneously hypertensive rats. The effects of essential oil of Angelica sinensis on SHR blood pressure and its mechanism were studied from the renin angiotensin-aldosterone system. Methods from October 2015 to April 2016, 48 SPF grade male SHR aged 8 weeks were randomly divided into model group. Low dose group, middle dose group, high dose group, ligustilide group, valsartan group, and 8 male Wistar rats with normal blood pressure of SPF grade were selected as normal group. The model group was not given any drugs, the low dose group, the middle dose group, the high dose group, the ligustilide group, the middle dose group, the ligustilide group, the middle dose group, the high dose group, and the ligustilide group, respectively. The rats in valsartan group were given Angelica volatile oil emulsion 12.6 mg 路kg ~ (-1) 路d ~ (-1) ~ (-1) ~ (37.0 mg 路kg ~ (-1)) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1)) by gavage, ligustilide solution (0.8 mg 路kg ~ (-1) ~ (-1)) 路d ~ (-1) ~ (-1), valsartan solution (51.4 mg 路kg ~ (-1)) 路d ~ (-1) 路L ~ (-1) for 4 weeks, and during the period of drug administration, rats in each group were fed with a standard diet of 0.80 mg 路kg ~ (-1) 路kg ~ (-1) 路L ~ (-1). Free drinking water. The changes of systolic blood pressure (SBP) were detected before administration and 4 weeks after administration. After 4 weeks of gastric perfusion, the rats were sacrificed to observe the pathological changes of myocardium by HE staining. The expression levels of angiotensin 鈪，

本文編號：1547077