本文關(guān)鍵詞： 小立碗蘚 PpAGO 基因克隆 表達(dá)分析　出處：《西北植物學(xué)報(bào)》2017年08期 　論文類型：期刊論文

【摘要】：該研究采用同源克隆的方法獲得了小立碗蘚AGO7蛋白編碼基因PpAGO7,對(duì)PpAGO7基因序列特征進(jìn)行生物信息學(xué)分析,并利用Real-time PCR方法分析PpAGO7基因的時(shí)空表達(dá)模式,為揭示小立碗蘚中PpAGO7基因的生物學(xué)功能提供依據(jù)。結(jié)果表明:PpAGO7基因的全長(zhǎng)cNDA為3 583bp,其中開放閱讀框3 363bp,編碼1 120個(gè)氨基酸,分子量123.38kD,等電點(diǎn)9.26,含有AGO蛋白典型的PAZ和PIWI結(jié)構(gòu)域;PpAGO7基因在小立碗蘚整個(gè)生活周期都有表達(dá),且在莖葉體生長(zhǎng)時(shí)期表達(dá)水平較高,但在原絲體時(shí)期表達(dá)水平較低。研究結(jié)果推測(cè)PpAGO7基因可能在莖葉體擬葉的生長(zhǎng)發(fā)育過(guò)程中起作用。
[Abstract]:In this study, AGO7 protein encoding gene PpAGO7 was obtained by homologous cloning. The sequence of PpAGO7 gene was analyzed by bioinformatics, and the temporal and spatial expression pattern of PpAGO7 gene was analyzed by Real-time PCR method. The results showed that the full length cNDA of the PpAGO7 gene was 3 583bp, and the open reading frame was 3 363bp, encoding 1 120 amino acids. Molecular weight 123.38kD, isoelectric point 9.26. the PAZ and PIWI domain PpAGO7, which contains AGO protein, was expressed in the whole life cycle of Moss, and the expression level of PpAGO7 was higher during the growth of stem and leaf. The results suggested that PpAGO7 gene might play an important role in the growth and development of stem and leaf mimicry.
【作者單位】： 江蘇師范大學(xué)生命科學(xué)學(xué)院;蘇州大學(xué)電子信息學(xué)院;
【基金】：基金項(xiàng)目:2015年江蘇省研究生創(chuàng)新工程(KYZZ15-0388) 江蘇省高校優(yōu)勢(shì)學(xué)科建設(shè)工程
【分類號(hào)】：Q943.2
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本文編號(hào)：1544629