擬南芥Trihelix轉錄因子基因家族研究
發(fā)布時間:2018-02-23 23:49
本文關鍵詞: 擬南芥 Trihelix 轉錄因子家族 進化 出處:《江蘇科技大學學報(自然科學版)》2017年02期 論文類型:期刊論文
【摘要】:Trihelix轉錄因子家族在植物生長發(fā)育與響應逆境脅迫等方面發(fā)揮著重要作用,但目前基于擬南芥全基因組水平鑒定和分析該基因家族的研究尚未見相關報道.利用生物信息學方法在擬南芥基因組數據庫中鑒定到Trihelix家族成員34個.通過序列聚類和功能結構域分析發(fā)現,該家族均含有高度保守的、特征性的Trihelix結構域;根據親緣關系遠近和結構域特點,將擬南芥Trihelix轉錄因子家族分為5個亞家族,基于MEME程序分析的保守基序與聚類分析結果具有較高的一致性.采用Int Act在線數據庫分析,發(fā)現擬南芥Trihelix轉錄因子家族各成員與其他蛋白存在互作關系.文中初步明確了擬南芥Trihelix轉錄因子家族成員的結構和功能特點、染色體分布、核定位信號及該家族蛋白與其他蛋白質的互作情況,為進一步揭示Trihelix轉錄因子家族的系統(tǒng)發(fā)育和生物學功能奠定了基礎.
[Abstract]:Trihelix transcription factor family plays an important role in plant growth and development and response to stress. However, there are no reports on the identification and analysis of the gene family based on the whole genome level of Arabidopsis thaliana. 34 members of the Trihelix family were identified by bioinformatics in the genome database of Arabidopsis thaliana. Clustering and functional domain analysis showed that, The Trihelix transcription factor family of Arabidopsis thaliana was divided into five subfamilies according to the genetic relationship and the characteristics of the domain. The conservative motifs based on MEME program are in good agreement with the results of clustering analysis. Int Act online database analysis is used to analyze the conserved motifs. The interaction between members of Arabidopsis Trihelix transcription factor family and other proteins was found. The structural and functional characteristics and chromosomal distribution of Arabidopsis Trihelix transcription factor family members were preliminarily clarified. The nuclear localization signal and the interaction between the family proteins and other proteins lay a foundation for further revealing the phylogenetic and biological functions of the Trihelix transcription factor family.
【作者單位】: 江蘇科技大學生物技術學院;
【基金】:江蘇科技大學研究生科技創(chuàng)新計劃項目(1252161418) 現代蠶桑產業(yè)技術體系項目(CARS-22) 公益性行業(yè)(農業(yè))科研專項項目(201403049)
【分類號】:Q943.2
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本文編號:1528089
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