發(fā)布時間：2018-02-21 20:05

  本文關鍵詞： 冬瓜 小西葫蘆黃花葉病毒 外殼蛋白基因 遺傳變異　出處：《園藝學報》2016年01期 　論文類型：期刊論文

【摘要】：小西葫蘆黃花葉病毒(Zucchini yellow mosaic virus,ZYMV)是危害廣東省冬瓜的常見病毒。摸索了冬瓜葉片總RNA最佳提取方法,通過基于ZYMV病毒外殼蛋白基因(coat protein gene,cp)序列的RT-PCR擴增,檢測采集自廣東省9個冬瓜產區(qū)的76份病毒侵染樣品的病毒田間發(fā)病率;將擴增產物克隆測序,利用MegAlign軟件構建系統(tǒng)發(fā)育進化樹分析cp基因遺傳變異與系統(tǒng)進化。結果表明,24份樣品檢測為陽性,田間發(fā)病率為31.59%;所有參試病毒分離物cp基因全長840 bp,編碼279個氨基酸的多肽;測序的16個分離物cp基因的核苷酸序列與推導蛋白的氨基酸序列相似性分別為98.1%~100%與96.1%~100%;所有廣東分離物的推導氨基酸序列基序保守,除GD121-9增加2個人類白細胞抗原外,其他均包含5個人類白細胞抗原及1個N糖基化位點,均包含保守potyv_CP功能域及與病毒蚜傳相關的結構域DAG三聯(lián)盒"Asp-Ala-Gly";系統(tǒng)進化分析表明,ZYMV病毒劃分為7個基因型,廣東分離物均屬于同一基因型Ⅰ,包含3個株系;廣東地區(qū)的16個分離物分為3組,無明顯地域選擇性�？傮w上廣東ZYMV-cp比較保守,分子變異較小。明確了廣東省冬瓜上ZYMV病毒的侵染情況及外殼蛋白基因的變異特點,為對其致病性和抗病毒基因工程等研究提供依據。
[Abstract]:Zucchini yellow mosaic virus (ZYMV) is a common virus that harms winter melon in Guangdong province. The best method for extracting total RNA from leaves of Chinese winter melon was explored, and the RT-PCR amplification was carried out based on the coat protein gene gene of ZYMV virus. The incidence of virus infection from 76 samples collected from 9 areas of winter melon production in Guangdong Province was detected, and the amplified products were cloned and sequenced. Phylogenetic tree was constructed with MegAlign software to analyze the genetic variation and phylogenetic variation of CP gene. The results showed that 24 samples were positive and the incidence was 31.59.The total length of CP gene was 840 BP, encoding 279 amino acid peptides. The similarity of nucleotide sequence of cp gene and deduced protein was 98.1100% and 96.1%, respectively. The deduced amino acid motifs of all Guangdong isolates were conserved, except for the addition of 2 human leukocyte antigens to GD121-9. The rest contained five human leukocyte antigens and one N-glycosylation site, all of which contained conserved potyv_CP functional domain and DAG triple box "Asp-Ala-Gly" associated with virus aphid transmission. Phylogenetic analysis showed that the virus was divided into seven genotypes. Guangdong isolates belong to the same genotype 鈪，

本文編號：1522758