本文關(guān)鍵詞： 先天性馬蹄內(nèi)翻足 GLI基因 維吾爾族 漢族 發(fā)病關(guān)系 新疆 兒童　出處：《山東醫(yī)藥》2016年42期 　論文類型：期刊論文

【摘要】：目的探討GLI3基因表達與新疆維吾爾族先天性馬蹄內(nèi)翻足(ICTEV)發(fā)病之間的關(guān)系。方法選擇新疆地區(qū)84例ICTEV患兒(維吾爾族51例,漢族33例)和39例足部創(chuàng)傷正常兒童(維吾爾族25例,漢族14例),分別采用實時熒光定量PCR、Western blot法檢測足部肌肉組織中的GLI3 mRNA及蛋白。結(jié)果觀察組足部肌肉組織中GLI3 mRNA及蛋白相對表達量分別為0.61±0.02、68.72±3.05,對照組分別為0.07±0.01、22.38±1.74,P均0.01;觀察組維吾爾族患兒足部肌肉組織中GLI3 mRNA及蛋白相對表達量分別為0.73±0.01、83.01±4.42,漢族患兒分別為0.40±0.02、56.85±3.50,P均0.05;對照組維吾爾族和漢族患兒足部肌肉組織中GLI3mRNA及蛋白相對表達量比較,P均0.05。結(jié)論 GLI3基因表達與新疆維吾爾族ICTEV的發(fā)病有一定關(guān)系,維吾爾族可能更易受GLI3基因表達水平的影響而導(dǎo)致ICTEV的發(fā)生。
[Abstract]:Objective to investigate the relationship between the expression of GLI3 gene and the incidence of congenital equinovarus equinovarus in Xinjiang Uygur. Methods 84 cases of ICTEV in Xinjiang (51 cases of Uygur) were selected. There were 33 cases of Han nationality) and 39 cases of normal children with foot trauma (25 cases of Uygur nationality). GLI3 mRNA and protein in muscle tissue of foot were detected by real-time fluorescence quantitative PCR Western blot. Results the relative expression of GLI3 mRNA and protein in the muscle of foot in the observation group was 0.61 鹵0.02, 68.72 鹵3.05, and that in the control group was 0.07 鹵0.01, 22.38 鹵1.74, respectively (P < 0.01). The relative expression of GLI3mRNA and protein in the muscle tissue of Uygur and Han nationality were 0.73 鹵0.01 鹵83.01 鹵4.42 and 0.40 鹵0.02 鹵56.85 鹵3.50 P respectively, while the relative expression of GLI3mRNA and protein in the muscle tissue of the Uygur and Han nationality in the control group were compared (P. Conclusion the expression of GLI3 gene is related to the pathogenesis of ICTEV in Xinjiang Uygur nationality. Uygur may be more susceptible to the GLI3 gene expression level and lead to the occurrence of ICTEV.
【作者單位】： 新疆醫(yī)科大學(xué)附屬中醫(yī)醫(yī)院;
【基金】：國家自然科學(xué)基金資助項目(81160215)
【分類號】：R726.8
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本文編號：1518733