本文關(guān)鍵詞： 胰腺癌 AsPC-細(xì)胞 誘騙受體基因 RNA干擾 放射敏感性　出處：《中國(guó)腫瘤生物治療雜志》2017年08期 　論文類型：期刊論文

【摘要】：目的:探討RNA干擾沉默誘騙受體3(decoy receptor 3,DcR3)基因?qū)σ认侔┘?xì)胞放射敏感性的影響及其相關(guān)機(jī)制。方法:構(gòu)建帶有DcR3 siRNA序列的穩(wěn)定表達(dá)質(zhì)粒,通過(guò)脂質(zhì)體轉(zhuǎn)染至胰腺癌AsPC-1細(xì)胞株,設(shè)對(duì)照組、siRNA(-)陰性對(duì)照組和DcR3 siRNA組,應(yīng)用Western blotting檢測(cè)AsPC-1細(xì)胞中DcR3表達(dá)的變化,平板克隆形成實(shí)驗(yàn)檢測(cè)轉(zhuǎn)染DcR3 siRNA后AsPC-1細(xì)胞放射敏感性的變化,流式細(xì)胞術(shù)檢測(cè)細(xì)胞凋亡,RT-PCR和Western blotting檢測(cè)Caspase-8、Caspase-3和PARP-1表達(dá)的變化。結(jié)果:DcR3 siRNA組細(xì)胞中DcR3蛋白表達(dá)水平較對(duì)照或siRNA(-)組明顯降低(均P0.01);DcR3 siRNA組的克隆形成率明顯低于對(duì)照或siRNA(-)組,其存活分?jǐn)?shù)(survival fraction,SF)降低、α/β比值升高(均P0.01);放射后DcR3 siRNA組腫瘤細(xì)胞凋亡率明顯高于對(duì)照或siRNA(-)組(均P0.01);轉(zhuǎn)染DcR3 siRNA后可以明顯上調(diào)Caspase-8、Caspase-3的表達(dá)和下調(diào)PARP-1的表達(dá)。結(jié)論:RNA干擾沉默DcR3基因通過(guò)激活凋亡因子Caspase-8和Caspase-3促進(jìn)細(xì)胞凋亡,從而增加胰腺癌細(xì)胞對(duì)放射的敏感性。
[Abstract]:Aim: to investigate the effect of RNA interference silencing receptor 3 decoy receptor 3 (DcR3) gene on radiosensitivity of pancreatic cancer cell line. Methods: stable expression plasmid with DcR3 siRNA sequence was constructed and transfected into AsPC-1 cell line of pancreatic carcinoma by liposome. Western blotting was used to detect the expression of DcR3 in AsPC-1 cells and the radiosensitivity of AsPC-1 cells transfected with DcR3 siRNA was detected by plate clone formation assay. RT-PCR and Western blotting were used to detect the expression of Caspase-8, Caspase-3 and PARP-1. Results the expression of DcR3 protein in the cells of the control group and the siRNA-treated group was significantly lower than that of the control or siRNA-treated group (P 0.01) and the clone formation rate of the siRNA group was significantly lower than that of the control group or siRNA-group. The survival fraction and 偽 / 尾 ratio of DcR3 siRNA group were significantly higher than those of control group or siRNA-group (P 0.01). After transfection of DcR3 siRNA, the expression of Caspase-8 and Caspase-3 and down-regulated expression of PARP-1 were significantly up-regulated. Conclusion the expression of Caspase-8 caspase-3 and down-regulated expression of PARP-1 can be down-regulated by DcR3 siRNA transfection (P < 0.01), and the apoptotic rate of tumor cells in DcR3 siRNA group after irradiation is significantly higher than that in control group or siRNA-group (P < 0.01). Silencing DcR3 gene promotes apoptosis by activating apoptosis factors Caspase-8 and Caspase-3. Thus increasing the radiosensitivity of pancreatic cancer cells.
【作者單位】： 湘南學(xué)院附屬醫(yī)院腫瘤中心;密蘇里大學(xué)醫(yī)學(xué)院Ellis
【基金】：湖南省自然科學(xué)基金資助項(xiàng)目(No.14JJ3136) 郴州市科技局科研基金資助項(xiàng)目(No.CZ2013096)~~
【分類號(hào)】：R735.9

【參考文獻(xiàn)】

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