本文關(guān)鍵詞： 高原 腺病毒 HAdV-B 病原分離 全基因測(cè)序　出處：《軍事醫(yī)學(xué)》2017年06期 　論文類型：期刊論文

【摘要】：目的探討高原地區(qū)55型腺病毒(HAdV-B55)基因變異特點(diǎn)。方法采集駐西藏某部隊(duì)55型腺病毒感染者咽拭子標(biāo)本,采用HEp-2分離病毒株,通過(guò)分片段PCR擴(kuò)增和測(cè)序獲得HAdV-B55毒株的全基因序列,通過(guò)與內(nèi)地毒株進(jìn)行序列比對(duì),發(fā)現(xiàn)其變異特點(diǎn)。結(jié)果從55型腺病毒感染者咽拭子標(biāo)本分離得到HAdV-B55毒株,命名為L(zhǎng)S89/Tibet/2016,通過(guò)PCR產(chǎn)物測(cè)序拼接,獲得了本次流行的HAdV-B55毒株的全基因序列,Gen Bank登錄號(hào)為KY002683。通過(guò)MEGA6軟件比對(duì),發(fā)現(xiàn)該病毒基因序列并未發(fā)生大片段重組,與內(nèi)地以往流行毒株(QS-DLL株)序列相似性為99.9%。結(jié)論本次分離出的病毒基因序列未發(fā)生大片段重組。本研究為揭示55型腺病毒的進(jìn)化特點(diǎn)及今后高原地區(qū)制訂疾病防控策略提供了依據(jù)。
[Abstract]:Objective to investigate the variation characteristics of adenovirus 55 (HAdV-B55) gene in plateau area. Methods the throat swabs of adenovirus type 55 infection in a military unit in Tibet were collected and the virus strains were isolated by HEp-2. The whole gene sequence of HAdV-B55 strain was obtained by fragment PCR amplification and sequencing. Results HAdV-B55 strain was isolated from throat swabs of adenovirus type 55 infection and named LS89/Tibet/2016. The whole gene sequence of HAdV-B55 strain was obtained by sequencing and splicing the PCR product. The accession number of Genin Bank was KY002683. The result was compared by MEGA6 software. It was found that there was no large fragment recombination in the gene sequence of the virus. The QS-DLL strain and the previous prevalent virus strain in mainland China. The sequence similarity is 99.9. Conclusion there is no large fragment recombination of the virus gene sequence isolated in this paper. This study provides a method to reveal the evolutionary characteristics of adenovirus type 55 and to formulate disease prevention and control strategies in the plateau region in the future. Basis
【作者單位】： 成都軍區(qū)疾病預(yù)防控制中心;成都軍區(qū)總醫(yī)院檢驗(yàn)科;
【基金】：全軍后勤“十二五”重大專項(xiàng)資助項(xiàng)目(AWS11L009) 軍隊(duì)“十二五”重點(diǎn)課題資助項(xiàng)目(BWS14J025) 國(guó)家自然科學(xué)基金資助項(xiàng)目(81301445) 全軍醫(yī)學(xué)科技青年培育項(xiàng)目(14QNP057) 上海市醫(yī)學(xué)生物防護(hù)重點(diǎn)實(shí)驗(yàn)室開放課題資助項(xiàng)目(SKLM1401)
【分類號(hào)】：R824

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