本文關(guān)鍵詞： 白細(xì)胞介素- 白細(xì)胞介素- 熱休克蛋白 腦海馬組織 敦煌石室大寶膠囊 衰老　出處：《中國(guó)老年學(xué)雜志》2017年16期 　論文類型：期刊論文

【摘要】：目的觀察以健脾補(bǔ)腎-滌痰祛瘀法為指導(dǎo)的敦煌石室大寶膠囊(DHDB)對(duì)衰老模型大鼠血清白細(xì)胞介素(IL)-4、IL-10含量和海馬區(qū)熱休克蛋白(HSP)86基因和蛋白表達(dá)的影響。方法受試動(dòng)物按隨機(jī)數(shù)字表法分為空白組(n=10)和模型組(n=50)。采用D-半乳糖(0.125 g/kg)皮下注射建立衰老模型并篩選符合衰老模型的大鼠,按隨機(jī)數(shù)字表法分為模型組、DHDB高、中、低劑量組(0.8、0.4、0.2 g/kg)和陽(yáng)性對(duì)照組(0.4 g/kg吡拉西坦),經(jīng)藥物干預(yù)后分別測(cè)定各組大鼠血清IL-4、IL-10含量和海馬區(qū)HSP86基因和蛋白表達(dá)水平。結(jié)果與空白組比較,模型組大鼠學(xué)習(xí)能力下降,血清IL-4含量顯著降低、IL-10含量顯著增高,海馬區(qū)HSP86基因和蛋白表達(dá)水平顯著降低(均P0.05);DHDB各劑量組大鼠學(xué)習(xí)能力提高,血清IL-4含量顯著增高而IL-10含量顯著降低,海馬區(qū)HSP86基因和蛋白表達(dá)水平顯著增高,且以高劑量DHDB作用為優(yōu)(P0.05)。結(jié)論 DHDB具有提高衰老大鼠學(xué)習(xí)記憶能力,調(diào)節(jié)血清IL-4、IL-10含量正常分泌及促進(jìn)海馬區(qū)HSP86基因和蛋白表達(dá)的作用。
[Abstract]:Objective to observe the effect of DHDBs of Dunhuang Shizhuangdabao capsule (DHDBs) on serum interleukin IL-4 in aging model rats guided by the method of invigorating spleen and kidney and removing phlegm from phlegm and removing blood stasis. Effects of IL-10 content and heat shock protein (HSPN) 86 gene and protein expression in hippocampal region. Methods the tested animals were randomly divided into two groups: control group (n = 10) and model group (n = 50). The aging model was established by subcutaneous injection of D-galactose 0.125 g / kg and the aging model was selected. According to the method of random number table, the model group was divided into three groups: model group with high, medium and low doses of 0.8g / kg 0.40.2g / kg) and positive control group with 0.4 g / kg Piracetam). Serum IL-4 IL-10 level and HSP86 gene and protein expression level in hippocampus were measured after drug intervention. Results compared with the blank group, the learning ability of the model group decreased. The level of serum IL-4 decreased significantly, the level of IL-10 increased significantly, and the expression of HSP86 gene and protein in hippocampus decreased significantly (all P 0.05). The learning ability of rats in each dose group of DHDB was improved, the content of serum IL-4 was significantly increased, the content of IL-10 was significantly decreased, and the expression of HSP86 gene and protein was significantly increased in hippocampus. Conclusion DHDB can improve learning and memory ability and regulate serum IL-4 in aging rats. Normal secretion of IL-10 and promotion of HSP86 gene and protein expression in hippocampus.
【作者單位】： 甘肅中醫(yī)藥大學(xué)敦煌醫(yī)學(xué)與轉(zhuǎn)化省部共建教育部重點(diǎn)實(shí)驗(yàn)室;甘肅中醫(yī)藥大學(xué)甘肅省中藥新產(chǎn)品創(chuàng)新工程重點(diǎn)實(shí)驗(yàn)室;
【基金】：敦煌醫(yī)學(xué)與轉(zhuǎn)化省部共建教育部重點(diǎn)實(shí)驗(yàn)室開(kāi)放基金(No.DHYX1213-009) 甘肅省科技攻關(guān)計(jì)劃項(xiàng)目(No.2GS064-A43-020-22) 蘭州市科技局計(jì)劃項(xiàng)目(No.06-2-87)
【分類號(hào)】：R285.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前8條

1 吳永杰;顧文勇;;加減補(bǔ)陽(yáng)還五湯對(duì)易卒中自發(fā)性高血壓大鼠海馬區(qū)一氧化氮的影響[J];中國(guó)比較醫(yī)學(xué)雜志;2006年08期

2 劉國(guó)安;鄭煒;楊紅;丁蘭;康文亮;;D-半乳糖處理小鼠抗氧化能力及肝和海馬區(qū)形態(tài)學(xué)變化的研究[J];西北師范大學(xué)學(xué)報(bào)(自然科學(xué)版);2012年03期

3 蔣犁;賈瑞;喬立興;陳平圣;;黃芪對(duì)新生鼠乏氧缺血腦損傷后海馬區(qū)神經(jīng)的保護(hù)及學(xué)習(xí)記憶能力的干預(yù)(英文)[J];中國(guó)臨床康復(fù);2006年07期

4 楊牧祥;武常生;于文濤;胡金寬;;醒腦啟智膠囊對(duì)血管性癡呆小鼠腦組織海馬區(qū)乙酰膽堿酯酶的影響[J];北京中醫(yī)藥大學(xué)學(xué)報(bào);2006年03期

5 陳亞亮,陸濤,馬育平,景朋,高秀來(lái),郭愛(ài)民;魚(yú)油制劑中DHA對(duì)小鼠海馬NOS陽(yáng)性神經(jīng)細(xì)胞表達(dá)的影響[J];解剖學(xué)研究;2001年01期

6 牛憲立;蘇良辰;姬可平;王曉明;魏妮娜;周放林;;黃連浸出液對(duì)腦缺血小鼠海馬區(qū)細(xì)胞總蛋白和SOD的影響[J];基因組學(xué)與應(yīng)用生物學(xué);2013年03期

7 俞靄瑤,王軍,陳明,陳兆和,韓麗萍,方家生;Ge132對(duì)老年鼠的促智作用[J];上海第二醫(yī)科大學(xué)學(xué)報(bào);1993年03期

8 譚展望;胡海燕;陳翔;王文花;張曉艷;楚生輝;;清心開(kāi)竅方皂苷對(duì)AD大鼠大腦皮層及海馬區(qū)Bax、Bcl-2、Aβ及βAPP表達(dá)的影響[J];中國(guó)中西醫(yī)結(jié)合雜志;2012年09期

相關(guān)碩士學(xué)位論文 前1條

1 徐雅嵐;模擬糖尿病并發(fā)抑郁癥環(huán)境下海馬區(qū)神經(jīng)血管單元體外模型的建立及中藥干預(yù)[D];湖南中醫(yī)藥大學(xué);2016年

，

本文編號(hào)：1492358