發(fā)布時間：2018-02-04 04:39

  本文關(guān)鍵詞： 水稻 HSP90 基因家族 表達 逆境　出處：《四川農(nóng)業(yè)大學(xué)》2016年博士論文　論文類型：學(xué)位論文

【摘要】：隨著近年來極端天氣的出現(xiàn)愈加頻繁,高溫、低溫、干旱及土壤鹽漬化等逆境脅迫對作物的生長、發(fā)育、產(chǎn)量的影響也越發(fā)凸顯。研究作物耐逆機制,培育耐逆品種成為當(dāng)前亟待解決的問題。HSP90家族蛋白是一類豐富的分子伴侶蛋白,并廣泛存在于真核生物中的保守蛋白。HSP90家族蛋白的主要功能主要是協(xié)助其它蛋白的正確折疊,并在逆境應(yīng)答網(wǎng)絡(luò)中發(fā)揮重要作用。本實驗中利用生物信息學(xué)的工具,發(fā)現(xiàn)并分析了日本晴中HSP90家族的9個OsHSP90蛋白(OsHSP90-1至OsHSP90-9).通過序列比對,我們在水稻HSP90家族蛋白中確定了三個基序。通過進化樹分析,將水稻HSP90家族蛋白分為三簇(Ⅰ,Ⅱ,Ⅲ)。本實驗在水稻10個組織中對這9個OsHSP90蛋白基因進行了表達量分析。結(jié)果表明在10個部位的組織中OsHSP90-4、OsHSP90-6和OsHSP90-7有較高的表達水平,而OsHSP90-5和OsHSP90-8的表達水平非常低。OsHSP90-1只特異性的在開花14天后的胚中表達。據(jù)文獻報道,在熱激和其它逆境脅迫下,熱激蛋白的表達會受到正向調(diào)控。但在本研究中,不同逆境脅迫條件下,水稻OsHSP90家族蛋白的表達模式卻是各式各樣的。在干旱、高鹽、低溫、熱激脅迫下OsHSP90-2和OsHSP90-4的表達受到了正向調(diào)控,在高鹽、干旱的脅迫下OsHSP90-3和OsHSP90-5的表達受到了負向調(diào)控。在干旱脅迫下OsHSP90-7和OsHSP90-9受到負向調(diào)控。與對照相比,在多種逆境脅迫下OsHSP90-6的表達量基本保持不變。我們分別用OsHSP90-2和OsHSP90-4轉(zhuǎn)化大腸桿菌,均能提高轉(zhuǎn)化菌株的細胞活性并提高對高溫、高鹽、干旱逆境環(huán)境的耐受力。我們分別用OsHsP90-2和OSHsP90-4轉(zhuǎn)化水稻,但只有OsHSP90-4的水稻轉(zhuǎn)化子對高溫的耐受力有明顯提高并增強水稻轉(zhuǎn)化子對ABA的敏感性。這些結(jié)果為進一步研究水稻OsHSP90基因家族提供了新的視角。
[Abstract]:With the increasing occurrence of extreme weather in recent years, high temperature, low temperature, drought and soil salinization and other stresses on crop growth, development, yield has become more and more prominent. Breeding of stress tolerance varieties has become a problem to be solved. HSP90 family protein is a kind of abundant molecular chaperone protein. The main function of the conserved protein. HSP90 family, which is widely present in eukaryotes, is to assist in the correct folding of other proteins. And play an important role in adversity response network. In this experiment, bioinformatics tools are used. Nine OsHSP90 proteins from the HSP90 family in Nippon were found and analyzed by sequence alignment from OsHSP90-1 to OsHSP90-9. We identified three motifs in rice HSP90 family proteins. By phylogenetic tree analysis, rice HSP90 family proteins were divided into three clusters (鈪，

本文編號：1489348