本文關(guān)鍵詞： 牛 溫度 牛精子相關(guān)抗原D 精子 睪丸細(xì)胞　出處：《中國(guó)畜牧獸醫(yī)》2017年05期 　論文類(lèi)型：期刊論文

【摘要】：為探討高溫對(duì)精子膜蛋白牛精子相關(guān)抗原11D(sperm associated antigen 11,SPAG11D)表達(dá)水平的影響,試驗(yàn)采用了實(shí)時(shí)熒光定量PCR和Western boltting方法檢測(cè)了牛睪丸原代細(xì)胞和娟姍牛、檳榔江牛、西門(mén)塔爾牛的正常精子及低活力精子中SPAG11D的表達(dá)。結(jié)果顯示,牛睪丸原代細(xì)胞在不同溫度(32、34、36、38和40℃)下培養(yǎng)24h,SPAG11D的mRNA及蛋白在36℃時(shí)表達(dá)水平最高,且高溫時(shí)表達(dá)水平比低溫時(shí)要高;培養(yǎng)48h時(shí),SPAG11D的mRNA表達(dá)基本和24h相一致,但蛋白表達(dá)水平在高溫時(shí)明顯下降。此外,檳榔江牛和娟姍牛低活力精子中SPAG11D的蛋白表達(dá)量比正常精子明顯降低,而在西門(mén)塔爾牛的精液中變化不明顯。
[Abstract]:To investigate the effect of high temperature on the expression of spermatozoa associated antigen (11DX) in bovine spermatozoa associated protein (SPAG11D). Real time fluorescence quantitative PCR and Western boltting were used to detect the primary cells of bovine testis and betel nut cattle. Expression of SPAG11D in normal and low motility spermatozoa of Simmental cattle. The results showed that primary bovine testicular cells were cultured at different temperatures for 24 hours. The expression level of mRNA and protein in SPAG11D was the highest at 36 鈩，

本文編號(hào)：1487717