本文關鍵詞： 家蠶核型多角體病毒(Bm NPV) gp基因 克隆 序列分析 微進化 廣西　出處：《南方農(nóng)業(yè)學報》2017年02期 　論文類型：期刊論文

【摘要】：【目的】了解廣西家蠶核型多角體病毒(Bombyx mori nucleopolyhedrovirus,Bm NPV)毒株gp64基因的保守性,并掌握其具體的突變位點,為研究核型多角體病毒(NPV)種內(nèi)的微進化模式提供參考依據(jù)。【方法】對廣西不同桑蠶產(chǎn)區(qū)的19株Bm NPV毒株進行gp64基因克隆與測序,分析完整開放閱讀框(ORF)序列的同源性和單核苷酸多態(tài)性,并構建基于gp64基因的遺傳進化樹�！窘Y果】廣西Bm NPV毒株的gp64基因ORF序列不一致,存在1590、1593和1599 nt三種類型,是由于第94~96位缺失GCG或第97~102位插入GCGCCG所致,且插入核苷酸突變僅在廣西Bm NPV毒株中出現(xiàn)。僅GXSL毒株與T3毒株的核苷酸及其推導氨基酸序列同源性均達100.0%,其余18株毒株與T3毒株的核苷酸序列同源性在98.5%~99.2%,其推導氨基酸序列同源性在98.7%~99.6%。19株廣西Bm NPV毒株被分為兩個亞群(cladeⅠ和cladeⅡ),其中11株獨立形成cladeⅡ,7株獨立形成cladeⅠ中的一個亞群;廣西毒株在多個位點出現(xiàn)同義核苷酸突變,呈一定的規(guī)律性�！窘Y論】廣西Bm NPV毒株的gp64基因具有遺傳多樣性,在遺傳進化上較獨立,插入突變顯示出地域特點。
[Abstract]:[objective] to understand the nuclear polyhedrosis virus (Bombyx mori nucleopolyhedrovirus) of Guangxi silkworm (Bombyx mori). The gp64 gene of Bm NPV strain was conserved and its specific mutation site was identified. [methods] 19 Bm NPV strains from different silkworm production areas in Guangxi were cloned and sequenced. To analyze the homology and single nucleotide polymorphism of complete open reading frame (ORF) sequence. The genetic and evolutionary tree based on gp64 gene was constructed. [results] the ORF sequence of gp64 gene of Bm NPV strain in Guangxi was different and there was 1590. The three types of 1593 and 1599nt were caused by the deletion of GCG at 94-96-bit or the insertion of GCGCCG at 97-102-bit. The inserted nucleotide mutation was only found in the Bm NPV strain in Guangxi. The nucleotide and deduced amino acid sequence homology between GXSL strain and T3 strain was 100.0%. The nucleotide sequence homology between the other 18 strains and T3 strain was 99.2%. The deduced amino acid sequence homology was 99.6.19 strains of Bm NPV in Guangxi were divided into two subgroups: clade 鈪，

本文編號：1482131