本文關(guān)鍵詞： 亞麻薺 抗病基因 NBS家族 基因簇 生物信息學(xué)　出處：《分子植物育種》2017年04期 　論文類型：期刊論文

【摘要】：NBS類抗病基因是植物中最大的一類抗病基因。亞麻薺是一種新型能源及特用油料作物,抗病、蟲(chóng)、雜草及逆境能力極強(qiáng)。然而,有關(guān)其抗性分子機(jī)理鮮有報(bào)道。本研究采用NB-ARC標(biāo)準(zhǔn)結(jié)構(gòu)域的HMM模型,對(duì)亞麻薺本地蛋白質(zhì)數(shù)據(jù)庫(kù)進(jìn)行檢索分析,在全基因組水平上鑒定NBS類抗病基因。結(jié)果表明亞麻薺基因組共有472個(gè)NBS類抗病基因,分布于17條染色體上,56%的基因成簇分布。所有串聯(lián)重復(fù)基因也都以基因簇的形式出現(xiàn),這表明串聯(lián)重復(fù)有利于基因簇的形成。系統(tǒng)發(fā)生樹(shù)分析顯示TIR-NBS-LRR(TNL)和CC-NBS-LRR(CNL)兩類亞家族的抗病基因可能在進(jìn)化過(guò)程中遵循不同的進(jìn)化路徑,導(dǎo)致其在應(yīng)對(duì)病原菌侵染時(shí)發(fā)揮不同的功能。本研究發(fā)現(xiàn)將為鑒定新的植物抗病基因和解析亞麻薺高抗病機(jī)制提供科學(xué)參考。
[Abstract]:NBS resistance genes are the largest resistance genes in plants. Flax is a new type of energy and special oil crops, disease resistance, insects, weeds and stress ability is very strong. The molecular mechanism of its resistance was rarely reported. In this study, HMM model of NB-ARC standard domain was used to search and analyze the native protein database of flax camelina. NBS resistance genes were identified at the whole genome level. The results showed that there were 472 NBS resistance genes distributed on 17 chromosomes. 56% of the genes were clustered. All tandem repeat genes also appeared in the form of clusters. The phylogenetic tree analysis showed that TIR-NBS-LRRRNL) and CC-NBS-LRRRN CNL). The disease resistance genes of the two subfamilies may follow different evolutionary pathways in the course of evolution. This study will provide a scientific reference for identifying new plant resistance genes and elucidating the mechanism of high resistance of flax chestnut.
【作者單位】： 山西農(nóng)業(yè)大學(xué);晉中學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31201266,31401430,31501323) 國(guó)家“948”項(xiàng)目(2014-Z39) 山西省煤基重點(diǎn)科技攻關(guān)項(xiàng)目(FT-2014-01) 山西省科技廳應(yīng)用基礎(chǔ)研究項(xiàng)目(201601D202060) 山西省高�？萍紕�(chuàng)新項(xiàng)目(2016171) 山西省高校大學(xué)生創(chuàng)新創(chuàng)業(yè)項(xiàng)目(2016413)共同資助
【分類號(hào)】：Q943.2;S435.659
【正文快照】： 植物在受到外界病原菌侵害時(shí)會(huì)產(chǎn)生一系列防御反應(yīng),大多數(shù)防御反應(yīng)是經(jīng)抗病基因(resistance gene,R基因)介導(dǎo)產(chǎn)生的。近年來(lái),已有100多種的R基因從眾多植物中被克隆出來(lái),根據(jù)其編碼蛋白的保守結(jié)構(gòu)域不同可將抗病基因分為不同的類型,其中家族成員數(shù)目最多的一類即為核苷酸結(jié)合，

本文編號(hào)：1473648