本文關鍵詞： 豬白細胞抗原 SPF大白豬 SPF長白豬 SLA II類等位基因 多態(tài)性　出處：《中國實驗動物學報》2017年04期 　論文類型：期刊論文

【摘要】：目的研究實驗用SPF大白豬和長白豬SLA II類基因的多態(tài)性。方法分別采集15頭SPF大白豬和22頭長白豬抗凝血,分離外周血淋巴細胞,提取總RNA,反轉錄后RT-PCR擴增DQB1、DRB1和DQA基因并進行測序,分析獲得的SLA II類等位基因序列多態(tài)性。結果 3個基因共獲得25個等位基因,包括8個DQB1,10個DRB1和7個DQA,全部獲得ISAG SLA命名委員會的官方命名,其中3個等位基因首次提交完整序列,命名為DQB1*02:12(KU754590)、DQB1*02:03(KU754591)和DRB1*06:07(KU754601),3個DQA等位基因為新發(fā)現等位基因。SPF大白豬和長白豬DQB1等位基因與外源性抗原結合的15個氨基酸中,共有5個氨基酸具有高度保守性;DRB1等位基因的16個外源性抗原識別位點中,僅1個位點高度保守;DQA等位基因19個抗原結合位點中,有11個高度保守。SLA II類基因氨基酸序列分子進化樹表明,3個基因分別聚為兩大類,與國外Yucatan小型豬具有較近的親緣關系,而與其他豬種未表現明顯遺傳距離相隔。結論成功鑒定了大白豬和長白豬的25個SLA II類等位基因,發(fā)現其具有較為豐富的多態(tài)性,所獲得SLA II類等位基因在其他品種豬也廣泛分布,具有多樣性,這一研究結果對大白豬和長白豬發(fā)展為經典實驗動物模型具有重要作用。
[Abstract]:Objective to study the polymorphism of SLA class II gene in SPF white pigs and Landrace pigs. Methods 15 SPF white pigs and 22 Landrace pigs were collected to isolate peripheral blood lymphocytes. Total RNAs were extracted, and DQB1hDRB1 and DQA genes were amplified by reverse transcription RT-PCR and sequenced. Results 25 alleles including 8 DQB1, 10 DRB1 and 7 DQA were obtained from 3 alleles. All of them were officially named by the ISAG SLA Nomenclature Committee, of which three alleles were first submitted to complete sequence, named DQB1 / 02: 12 KU754590). DQB102: 03KU754591 and DRB1 06: 07KU754601). The three DQA alleles were the newly discovered alleles. DQB1 alleles were highly conserved in 15 amino acids of DQB1 alleles combined with exogenous antigens. Of the 16 exogenous antigen recognition sites in the DRB1 allele, only one was highly conserved. Among the 19 antigen-binding sites of DQA alleles, 11 highly conserved. SLA II gene amino acid sequence molecular evolutionary tree showed that 3 genes were clustered into two categories respectively. There is a close relationship with foreign Yucatan miniature pigs. Conclusion 25 SLA class II alleles of large white pigs and Landrace pigs were successfully identified and found to be rich in polymorphism. The obtained alleles of SLA II are also widely distributed in other breeds of pigs and have diversity, which plays an important role in the development of large white pigs and Landrace pigs into classical experimental animal models.
【作者單位】： 牡丹江師范學院生命科學與技術學院;中國農業(yè)科學院哈爾濱獸醫(yī)研究所獸醫(yī)生物技術國家重點實驗室;甘肅農業(yè)大學動物科學技術學院;
【基金】：國家自然科學基金項目(編號:.31502039)
【分類號】：Q95-33
【正文快照】： (1.College of Life Sciences and Technology,Mudanjiang Normal University,Mudanjiang 157011,China;2.StateKey Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy ofAgricultural Sciences(CAAS),Harbin 150069;3.College o

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