本文關(guān)鍵詞： 電針 超前性光暗周期轉(zhuǎn)移 節(jié)律 SCN 鐘基因　出處：《中山大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年02期 　論文類型：期刊論文

【摘要】：為了探索電針重置超前性光暗周期轉(zhuǎn)移模型小鼠的時(shí)相特征及視交叉上核(Suprachiasmatic nucleus,SCN)多種節(jié)律相關(guān)基因及轉(zhuǎn)錄因子的表達(dá),將符合篩選標(biāo)準(zhǔn)的44只C57BL/6J小鼠完全隨機(jī)分為空白組(n=10)、模型組(n=12)、捆綁組(n=12)和電針組(n=10)4組。其中,模型組、捆綁組和電針組運(yùn)用超前性光暗周期轉(zhuǎn)移法進(jìn)行造模,連續(xù)10 d。造模結(jié)束后,模型組小鼠于ZT18時(shí)相點(diǎn)處死取材;電針組小鼠恢復(fù)正常光暗交替(Light and Dark,LD)狀態(tài),并于ZT16時(shí)相點(diǎn)選取"肝俞"和"至陽(yáng)"穴進(jìn)行電針治療,連續(xù)3次,每天1次;捆綁組采用與電針組相同的方法進(jìn)行平行捆綁。捆綁及治療結(jié)束后,處死動(dòng)物并剝?nèi)CN,采用PCR Array檢測(cè)各組動(dòng)物SCN內(nèi)節(jié)律相關(guān)基因及部分節(jié)律相關(guān)轉(zhuǎn)錄因子相對(duì)表達(dá)量。實(shí)驗(yàn)結(jié)果顯示:(1)電針重置晝夜節(jié)律結(jié)果:造模后,模型組、捆綁組及電針組與造模前比較峰相位、起始活動(dòng)時(shí)間均顯著超前,晝夜活動(dòng)節(jié)律周期縮短(P0.05);再同步期,電針組峰相位、起始活動(dòng)時(shí)間后移,與造模期及空白組比較具有統(tǒng)計(jì)學(xué)差異(P0.05);再同步第1天及第2天電針組小鼠的晝夜活動(dòng)節(jié)律周期與空白組和造模前比較有統(tǒng)計(jì)學(xué)差異(P0.05),再同步第3天與空白組和造模前比較無(wú)統(tǒng)計(jì)學(xué)差異(P0.05)。(2)SCN節(jié)律相關(guān)基因及轉(zhuǎn)錄因子變化:與空白組比較,模型組小鼠的SCN節(jié)律相關(guān)基因表達(dá)上調(diào)8個(gè)(Aanat、Crx、Epo、Nkx2-5、Pax4、Prf1、Rora、Stat5a),下調(diào)4個(gè)(Egr1、Per1、Per3、Prokr2);與模型組比較,捆綁組節(jié)律相關(guān)基因上調(diào)3個(gè)(Esrra、Mat2a、Per3),下調(diào)11個(gè)(Cartpt、Crx、Epo、Kcnma1、Mtnr1b、Nkx2-5、Nms、Pax4、Prf1、Prkacb、Prkca);與模型組比較,電針組節(jié)律相關(guān)基因上調(diào)6個(gè)(Egr1、Esrra、Mat2a、Per1、Per3、Prokr2),下調(diào)21個(gè)(Aanat、Arntl、Cartpt、Crx、Csnk1e、Epo、Kcnma1、Mtnr1b、Myod1、Nkx2-5、Nms、Opn3、Pax4、Prf1、Prkacb、Prkca、Prkcb、Prokr2、Rora、Rorb、Slc9a3、Tgfb1)。以上實(shí)驗(yàn)結(jié)果提示電針可縮短超前性光暗周期轉(zhuǎn)移小鼠的晝夜節(jié)律重置時(shí)間,加速紊亂晝夜節(jié)律的恢復(fù),這可能是通過(guò)對(duì)SCN內(nèi)Per1、Egr1、Aanat、Prokr2等節(jié)律相關(guān)基因的調(diào)控作用實(shí)現(xiàn)的。
[Abstract]:To explore the temporal characteristics and Suprachiasmatic nucleus of the suprachiasmatic nucleus of the supraoptic chiasma in an electroacupuncture resetting model mouse model of light and dark cycle transfer. 44 C57BL / 6J mice, which met the screening criteria, were randomly divided into two groups: control group (n = 10) and model group (n = 12). The model group, the binding group and the electroacupuncture group were established by the method of light and dark cycle transfer for 10 days. The mice in the model group were killed at the phase point of ZT18. The mice in the electroacupuncture group recovered to the normal light and dark alternating light and and LDD state, and selected "Ganshu" and "Zhiyang" acupoints at the ZT16 phase point for 3 consecutive times. Once a day; The animals in the binding group were subjected to parallel binding in the same way as those in the electroacupuncture group. After the binding and treatment, the animals were killed and the SCN was stripped. PCR Array was used to detect the relative expression of Rhythm related genes and some Rhythm related transcription factors in SCN of each group. The results showed that the electroacupuncture reset circadian rhythm: after modeling. In model group, binding group and electroacupuncture group, the peak phase was significantly earlier than that before model making, and the circadian cycle of activity was shortened (P 0.05). In resynchronization period, peak phase and initial activity time of electroacupuncture group moved backward, there was a statistical difference compared with model making period and blank group (P 0.05). The circadian activity rhythm of mice in the electroacupuncture group on day 1 and day 2 was significantly different from that in the blank group and before modeling (P0.05). On the 3rd day of resynchronization, there was no significant difference between the control group and the control group and before model making. There was no significant difference in the changes of the Rhythm related genes and transcription factors between the control group and the control group. The expression of SCN rhythm related genes in the model group was up-regulated. 4 Egr1, Per1, Per3, Prokr2a were down-regulated; Compared with the model group, the Rhythm related genes in the binding group were up-regulated by 3 Esrragnon Mat2aP3 and down regulated by 11 CartptTX CrxEpoxEpo-Kcnma1. Mtnr1b1, Nkx2-5, Pax4, Prf1, Prkacb1, Prkcaanus; Compared with the model group, the rhythm related genes of electroacupuncture group were up-regulated by 6 Egr1 Egr1, Esrrag, Mat2a, Per1, Per3, Prokr2, and down-regulated by 21 Aanat. Arntl-CartptCsnk1eCsnk1eCnma1Mtnr1b (Mtnr1b) Myod1Nkx2-5 (NMSS) Opn3Pax4. Prf1,Prkacb,Prkca,Prkcb,Prokr2,Rora,Rorb,Slc9a3. The above results suggest that electroacupuncture can shorten the time of circadian rhythm reset and accelerate the recovery of abnormal circadian rhythm in mice with light and dark cycle metastasis, which may be through the treatment of Per1 in SCN. The regulation of Rhythm related genes such as Egr1 Aanatron Prokr2 was realized.
【作者單位】： 成都中醫(yī)藥大學(xué);
【基金】：國(guó)家自然基金面上項(xiàng)目(81373739) 四川省教育廳項(xiàng)目(15ZB0089)
【分類號(hào)】：R245
【正文快照】： divided into 4 groups which were the blank group(n=10),the model group(n=12),the bindinggroup(n=12)and the electro-acupuncture group(n=10).We housed mice in LD cycle for 10 days.The control group was kept 12 h∶12 h LD of 20 days,and then SCN collected a

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