本文關(guān)鍵詞： 肝細(xì)胞癌 BTG 總生存期 免疫組化 預(yù)后　出處：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2017年07期 　論文類(lèi)型：期刊論文

【摘要】：目的探討B(tài)細(xì)胞異位基因2(B cell translocation gene 2,BTG2)在原發(fā)性肝細(xì)胞癌中的表達(dá)及與術(shù)后生存期之間的關(guān)系。方法共納入125例可手術(shù)切除的原發(fā)性肝細(xì)胞癌患者,其中有完整隨訪(fǎng)記錄101例;用免疫組化方法檢測(cè)患者腫瘤組織中BTG2蛋白的表達(dá),并分為3組:BTG2高表達(dá)組,BTG2蛋白中表達(dá)(++)或強(qiáng)表達(dá)(+++);BTG2低表達(dá)組,BTG2蛋白低表達(dá)(+);BTG2陰性組,BTG2蛋白無(wú)表達(dá)(-)。分析BTG2表達(dá)水平與臨床病理因素和術(shù)后總生存期的相關(guān)性。選取12對(duì)癌與癌旁配對(duì)組織,用RT-PCR檢測(cè)其BTG2表達(dá)水平。結(jié)果 125例癌組織中,BTG2陰性組、低表達(dá)組和高表達(dá)組分別為106、13、6例;癌旁組織中,BTG2陰性組、低表達(dá)組和高表達(dá)組分別為41、14、22例,癌與癌旁組織BTG2表達(dá)差異有統(tǒng)計(jì)學(xué)意義(χ~2=28.102,P0.001)。RT-PCR檢測(cè)結(jié)果顯示12對(duì)癌與癌旁配對(duì)組織中,癌組織BTG2表達(dá)水平低于癌旁的有8例。癌栓發(fā)生與BTG2表達(dá)有明顯相關(guān)性(χ~2=8.305,P=0.013),其中低表達(dá)組癌栓發(fā)生率最高。BTG2低表達(dá)組中位總生存期為36個(gè)月(95%CI:8.437~63.564),而高表達(dá)和陰性表達(dá)組未達(dá)到中位總生存期(Log-Rankχ~2=4.512,P=0.105)。亞組分析表明,BTG2高表達(dá)組患者總生存期顯著長(zhǎng)于低表達(dá)組(χ~2=4.266,P=0.039),而高表達(dá)與陰性組(χ~2=2.729,P=0.099)以及低表達(dá)與陰性組(χ~2=1.400,P=0.237)之間差異沒(méi)有統(tǒng)計(jì)學(xué)意義。逐步Cox回歸分析結(jié)果顯示,分化程度、肝硬化、癌栓和轉(zhuǎn)移情況是總生存期的獨(dú)立預(yù)后因素。結(jié)論 BTG2蛋白在原發(fā)性肝細(xì)胞癌中的表達(dá)呈低表達(dá),并且BTG2蛋白的高表達(dá)提示肝癌患者預(yù)后較好,可作為肝細(xì)胞癌的潛在預(yù)后指標(biāo)。
[Abstract]:Objective to investigate the effect of B cells (B cell translocation 2 ectopic gene gene 2, BTG2) expression in primary hepatocellular carcinoma and the relationship between the survival period and after operation. Methods a total of 125 patients with resectable hepatocellular carcinoma patients, which have complete follow-up records of 101 cases; the expression of BTG2 protein using immunohistochemical method in tumor tissue, and divided into 3 groups: BTG2 high expression group, the expression of BTG2 protein in (+ +) or (+ + +); the strong expression of BTG2 low expression group and low expression of BTG2 (+); BTG2 negative group, BTG2 protein expression (-) correlation analysis. The expression level of BTG2 and clinical pathological factors and postoperative overall survival. Select 12 of cancer and adjacent tissue, the expression level of BTG2 was detected by RT-PCR. Results 125 cases of cancer tissues, BTG2 negative group, low expression group and high expression group were 106,13,6; tumor tissues, BTG2 negative group low expression and high expression group. Group were 41,14,22, carcinoma and adjacent tissue BTG2 expression was statistically significant (~2=28.102, P0.001).RT-PCR showed that 12 of cancer and adjacent tissues, the level of the 8 cases was lower than that in adjacent cancer tissues. The expression of BTG2 tumor thrombus occurrence and the expression of BTG2 is significantly correlated (x ~2=8.305, P=0.013), the highest incidence of cancer embolus group.BTG2 low expression group median overall survival was 36 months (95%CI:8.437~63.564), the low expression and high expression and negative expression group did not reach the median overall survival (Log-Rank x ~2=4.512, P=0.105). Subgroup analysis showed that high expression of BTG2 in patients with overall survival significantly longer than the low expression group (~2=4.266, P=0.039), and the high expression and negative group (~2=2.729, P=0.099) and low expression and negative group (~2=1.400, P=0.237) between the difference was not statistically significant. Gradually the results were analyzed with Cox regression showed that the degree of differentiation, cirrhosis, and cancer embolus Metastasis is an independent prognostic factor of overall survival. Conclusion the expression of BTG2 protein is low in primary hepatocellular carcinoma, and the high expression of BTG2 indicates that the prognosis of HCC is good. It can be used as a potential prognostic indicator for hepatocellular carcinoma.

【作者單位】： 第三軍醫(yī)大學(xué)大坪醫(yī)院野戰(zhàn)外科研究所腫瘤中心;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81572959)~~
【分類(lèi)號(hào)】：R735.7
【正文快照】： 原發(fā)性肝癌是我國(guó)最常見(jiàn)的腫瘤之一,居所有腫瘤發(fā)病率的第4位,死亡率居第3位[1]。肝癌按細(xì)胞分型可分為肝細(xì)胞癌、膽管細(xì)胞癌及混合型肝癌,其中肝細(xì)胞癌最常見(jiàn)。肝細(xì)胞癌(hepatocellular carcinoma,HCC)是預(yù)后較差的腫瘤之一,約90%的患者有慢性乙型肝炎病史。由于只有10%~20%

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