本文關鍵詞： 肝細胞癌 BTG 總生存期 免疫組化 預后　出處：《第三軍醫(yī)大學學報》2017年07期 　論文類型：期刊論文

【摘要】：目的探討B(tài)細胞異位基因2(B cell translocation gene 2,BTG2)在原發(fā)性肝細胞癌中的表達及與術后生存期之間的關系。方法共納入125例可手術切除的原發(fā)性肝細胞癌患者,其中有完整隨訪記錄101例;用免疫組化方法檢測患者腫瘤組織中BTG2蛋白的表達,并分為3組:BTG2高表達組,BTG2蛋白中表達(++)或強表達(+++);BTG2低表達組,BTG2蛋白低表達(+);BTG2陰性組,BTG2蛋白無表達(-)。分析BTG2表達水平與臨床病理因素和術后總生存期的相關性。選取12對癌與癌旁配對組織,用RT-PCR檢測其BTG2表達水平。結果 125例癌組織中,BTG2陰性組、低表達組和高表達組分別為106、13、6例;癌旁組織中,BTG2陰性組、低表達組和高表達組分別為41、14、22例,癌與癌旁組織BTG2表達差異有統(tǒng)計學意義(χ~2=28.102,P0.001)。RT-PCR檢測結果顯示12對癌與癌旁配對組織中,癌組織BTG2表達水平低于癌旁的有8例。癌栓發(fā)生與BTG2表達有明顯相關性(χ~2=8.305,P=0.013),其中低表達組癌栓發(fā)生率最高。BTG2低表達組中位總生存期為36個月(95%CI:8.437~63.564),而高表達和陰性表達組未達到中位總生存期(Log-Rankχ~2=4.512,P=0.105)。亞組分析表明,BTG2高表達組患者總生存期顯著長于低表達組(χ~2=4.266,P=0.039),而高表達與陰性組(χ~2=2.729,P=0.099)以及低表達與陰性組(χ~2=1.400,P=0.237)之間差異沒有統(tǒng)計學意義。逐步Cox回歸分析結果顯示,分化程度、肝硬化、癌栓和轉移情況是總生存期的獨立預后因素。結論 BTG2蛋白在原發(fā)性肝細胞癌中的表達呈低表達,并且BTG2蛋白的高表達提示肝癌患者預后較好,可作為肝細胞癌的潛在預后指標。
[Abstract]:Objective to investigate the effect of B cells (B cell translocation 2 ectopic gene gene 2, BTG2) expression in primary hepatocellular carcinoma and the relationship between the survival period and after operation. Methods a total of 125 patients with resectable hepatocellular carcinoma patients, which have complete follow-up records of 101 cases; the expression of BTG2 protein using immunohistochemical method in tumor tissue, and divided into 3 groups: BTG2 high expression group, the expression of BTG2 protein in (+ +) or (+ + +); the strong expression of BTG2 low expression group and low expression of BTG2 (+); BTG2 negative group, BTG2 protein expression (-) correlation analysis. The expression level of BTG2 and clinical pathological factors and postoperative overall survival. Select 12 of cancer and adjacent tissue, the expression level of BTG2 was detected by RT-PCR. Results 125 cases of cancer tissues, BTG2 negative group, low expression group and high expression group were 106,13,6; tumor tissues, BTG2 negative group low expression and high expression group. Group were 41,14,22, carcinoma and adjacent tissue BTG2 expression was statistically significant (~2=28.102, P0.001).RT-PCR showed that 12 of cancer and adjacent tissues, the level of the 8 cases was lower than that in adjacent cancer tissues. The expression of BTG2 tumor thrombus occurrence and the expression of BTG2 is significantly correlated (x ~2=8.305, P=0.013), the highest incidence of cancer embolus group.BTG2 low expression group median overall survival was 36 months (95%CI:8.437~63.564), the low expression and high expression and negative expression group did not reach the median overall survival (Log-Rank x ~2=4.512, P=0.105). Subgroup analysis showed that high expression of BTG2 in patients with overall survival significantly longer than the low expression group (~2=4.266, P=0.039), and the high expression and negative group (~2=2.729, P=0.099) and low expression and negative group (~2=1.400, P=0.237) between the difference was not statistically significant. Gradually the results were analyzed with Cox regression showed that the degree of differentiation, cirrhosis, and cancer embolus Metastasis is an independent prognostic factor of overall survival. Conclusion the expression of BTG2 protein is low in primary hepatocellular carcinoma, and the high expression of BTG2 indicates that the prognosis of HCC is good. It can be used as a potential prognostic indicator for hepatocellular carcinoma.

【作者單位】： 第三軍醫(yī)大學大坪醫(yī)院野戰(zhàn)外科研究所腫瘤中心;
【基金】：國家自然科學基金面上項目(81572959)~~
【分類號】：R735.7
【正文快照】： 原發(fā)性肝癌是我國最常見的腫瘤之一,居所有腫瘤發(fā)病率的第4位,死亡率居第3位[1]。肝癌按細胞分型可分為肝細胞癌、膽管細胞癌及混合型肝癌,其中肝細胞癌最常見。肝細胞癌(hepatocellular carcinoma,HCC)是預后較差的腫瘤之一,約90%的患者有慢性乙型肝炎病史。由于只有10%~20%

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