本文關(guān)鍵詞： 小麥 抽穗期 基因定位 SSR BSA　出處：《麥類作物學(xué)報》2016年11期 　論文類型：期刊論文

【摘要】：為定位小麥品系XN6426抽穗期相關(guān)基因,在可控溫室(溫度16~25℃,日光照≥16h)和田間分別種植XN6426×京411F2代群體、早抽穗親本XN6426和晚抽穗親本京411,分析F2群體抽穗期表型,得出該表型由兩對基因控制。構(gòu)建溫室條件下F2群體的極端早抽穗和極端晚抽穗期DNA池(BSA法),采用小麥90KSNP芯片分析得出早晚抽穗期池間差異SNP位點在不同染色體上的分布頻率和相應(yīng)密集區(qū)域;在5A染色體上篩選出雙親和早晚抽穗期池間有多態(tài)性且分布在差異SNP位點密集區(qū)域附近的SSR標(biāo)記Xbarc151和Xwmc327,這兩對標(biāo)記檢測群體的基因型與其抽穗期表型之間極顯著負(fù)相關(guān)。檢測Xbarc151、Xwmc327以及親本間有多態(tài)性的標(biāo)記Xgwm186和Xwmc96在F2群體內(nèi)的基因型,并結(jié)合群體相應(yīng)抽穗期表型,利用復(fù)合區(qū)間作圖法,在5A染色體標(biāo)記Xbarc151和Xwmc327之間檢測到了1個抽穗期相關(guān)QTL位點qHD-5A-1,距離兩標(biāo)記的遺傳距離分別為1.00cM和11.49cM,LOD值為3.68,貢獻(xiàn)率為8.07%,結(jié)合前人結(jié)果初步確定該位點與Vrn-A1位點不同,可能為已知抽穗期相關(guān)基因的未知等位變異或新基因位點。
[Abstract]:For the location of wheat lines XN6426 heading related gene, in a controlled greenhouse (temperature 16~25, sunlight is no less than 16h) and in the field were planted XN6426 * Beijing 411F2 generation group, early heading parent XN6426 and late heading parent Beijing 411, F2 group analysis of phenotype, the phenotype was controlled by two pairs of genes to construct F2. The group under greenhouse conditions of extreme early heading and extreme late heading date in the DNA pool (BSA), using wheat 90KSNP chip analysis of distribution frequency differences between SNP sooner or later heading cell sites in different chromosomes and corresponding dense regions on chromosome 5A; selected parents and sooner or later heading stage between the polymorphism and the distribution of pool the difference in SNP Xbarc151 and Xwmc327 SSR marker loci concentrated near the area, between the genotypes on the two groups with markers of phenotype significantly negatively correlated. Detection of Xbarc151, Xwmc327 and polymorphisms between the parent The markers Xgwm186 and Xwmc96 in the F2 group within the group and combined with the corresponding genotype, phenotype, using composite interval mapping method between 5A chromosome markers Xbarc151 and Xwmc327 detected 1 QTL heading loci qHD-5A-1, the genetic distance of two marker distance were 1.00cM and 11.49cM, LOD value was 3.68. The contribution rate of 8.07%, with the previous results to determine the site and Vrn-A1 site, may be heading the unknown genes related to known alleles or loci.

【作者單位】： 西北農(nóng)林科技大學(xué)農(nóng)學(xué)院;寧夏農(nóng)林科學(xué)院農(nóng)作物研究所;
【基金】：國家重點基礎(chǔ)研究發(fā)展計劃(973計劃)項目(2104CB138102) 陜西省重點科技創(chuàng)新團(tuán)隊計劃項目(2014KCT-25) 寧夏農(nóng)業(yè)育種專項(2013NYYZ02) 寧夏農(nóng)林科學(xué)院科技創(chuàng)新先導(dǎo)資金對外科技合作專項-小麥品質(zhì)與抗逆性相關(guān)的多重PCR分子標(biāo)記體系引進(jìn)與利用;寧夏農(nóng)林科學(xué)院科技創(chuàng)新先導(dǎo)資金基礎(chǔ)研究項目(NKYJ-14-29) 西北農(nóng)林科技大學(xué)唐仲英育種基金項目
【分類號】：S512.1
【正文快照】： of the polymorphism SNP loci,and the genotype detected by the two SSR markers was significantlynegative correlated with the phenotype of heading.The genotype detected by Xbarc151,Xwmc327andthe two markers Xgwm186,Xwmc96which were polymorphism between the，

本文編號：1451358