本文關(guān)鍵詞： Bt棉 AM真菌 基因表達(dá) 酶活性　出處：《華中農(nóng)業(yè)大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：AM真菌廣泛存在于土壤生態(tài)系統(tǒng)中,能與80%的陸生植物品種形成共生關(guān)系,對(duì)宿主植物礦質(zhì)營(yíng)養(yǎng)吸收(特別是磷)具有顯著促進(jìn)作用,同時(shí)AM真菌從宿主植物獲得碳源。AM真菌與宿主植物的營(yíng)養(yǎng)交換主要在共生結(jié)構(gòu)體—叢枝界面進(jìn)行,由一系列功能基因表達(dá)及酶的催化完成。磷轉(zhuǎn)運(yùn)蛋白基因Gint PT、堿性磷酸酶基因Gint ALP、單糖轉(zhuǎn)運(yùn)蛋白基因Gint MST2及堿性磷酸酶、酸性磷酸酶和脲酶在物質(zhì)交換過程中發(fā)揮重要作用。AM真菌對(duì)宿主植物品種及環(huán)境條件的改變敏感。本研究通過盆栽實(shí)驗(yàn),研究了我國(guó)廣泛種植的5種轉(zhuǎn)Bt基因棉對(duì)AM真菌R.irregularis共生特性、功能基因表達(dá)及關(guān)鍵酶活性的影響。得出的主要結(jié)論如下:⑴Bt棉對(duì)R.irregularis共生性有明顯的影響。除中41外,其它4種Bt棉根段侵染率、侵染強(qiáng)度、叢枝豐度均低于對(duì)應(yīng)的常規(guī)棉;Bt棉晉26、晉44和GK12叢枝面積低于其對(duì)應(yīng)的常規(guī)棉;中41和s GK321叢枝面積與對(duì)應(yīng)常規(guī)棉無(wú)顯著差異。⑵Bt棉對(duì)侵染根段內(nèi)AM真菌Gint PT、Gint ALP、Gint MST2基因表達(dá)量有明顯影響。除個(gè)別時(shí)期外,Bt棉晉44、晉26、中41根段中Gint PT、Gint ALP、Gint MST2表達(dá)量低于對(duì)應(yīng)常規(guī)棉;GK12根段中AM真菌基因表達(dá)量與其親本泗棉3號(hào)差異不顯著;而s GK321根段中Gint PT、Gint ALP、Gint MST2的表達(dá)量高于常規(guī)棉石遠(yuǎn)321。⑶Bt棉對(duì)共生體內(nèi)堿性磷酸酶、酸性磷酸酶和脲酶活性有顯著影響。除GK12外,其它Bt棉共生根段內(nèi)堿性磷酸酶、酸性磷酸酶和脲酶活性表現(xiàn)為顯著低于相應(yīng)的常規(guī)棉或無(wú)顯著性差異。⑷接種R.irregularis顯著促進(jìn)Bt棉和常規(guī)棉對(duì)磷的吸收,提高了宿主植物生物量,對(duì)氮吸收沒有顯著影響;Bt棉和常規(guī)棉間氮磷含量及生物量無(wú)顯著的差異。
[Abstract]:AM fungi exist widely in the soil ecosystem and can form symbiotic relationship with 80% terrestrial plant varieties and play a significant role in promoting mineral nutrient uptake (especially phosphorus) of host plants. At the same time, the nutrient exchange between AM fungi and host plants was mainly carried out at the interface between symbiotic structure and branchlets. Phosphorous transporter gene Gint PTand alkaline phosphatase gene Gint ALP were expressed by a series of functional genes and catalyzed by enzymes. Monosaccharide transporter gene Gint MST2 and alkaline phosphatase. Acid phosphatase and urease play an important role in the process of substance exchange. AM fungi are sensitive to the change of host plant varieties and environmental conditions. The symbiotic characteristics of five transgenic BT cotton varieties against AM fungi R.irregularis were studied. The main conclusions are as follows: 1 BT cotton has a significant effect on R. irregularis symbiosis. The other four BT cotton had lower infection rate, infection intensity and branching abundance than the corresponding conventional cotton. The acreage of BT cotton Jin26, Jin44 and GK12 were lower than their corresponding conventional cotton. There was no significant difference between Zhong41 and s GK321 branch area and the corresponding conventional cotton. 2Bt cotton had no significant difference on AM fungus Gint ALP in infecting root segment. The expression of Gint MST2 gene was significantly affected except in a few periods, except in Jin44, Jin26, and Gint ALP in 41 segments of BT. The expression of Gint MST2 was lower than that of normal cotton. There was no significant difference between the gene expression of AM fungi and its parent Simian 3 in root segment of GK12. However, the expression of Gint MST2 in the root segment of s GK321 was higher than that in normal cotton Shiyuan 321.3Bt cotton. The activities of acid phosphatase and urease were significantly affected, except for GK12, alkaline phosphatase was found in the symbiotic root segment of BT cotton. The activity of acid phosphatase and urease was significantly lower than that of normal cotton or no significant difference .4 inoculated with R. irregularis significantly promoted the uptake of phosphorus by BT cotton and conventional cotton. The biomass of host plants was increased and nitrogen uptake was not significantly affected. There was no significant difference in nitrogen and phosphorus content and biomass between BT cotton and conventional cotton.
【學(xué)位授予單位】：華中農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S562

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