本文關(guān)鍵詞：毛竹TIPs基因家族成員組織表達(dá)模式研究　出處：《林業(yè)科學(xué)研究》2016年04期 　論文類型：期刊論文

  更多相關(guān)文章： 毛竹 液泡膜內(nèi)在水通道蛋白 表達(dá)分析

【摘要】：[目的]通過研究毛竹TIPs的分子特征和表達(dá)模式,為揭示逆境脅迫條件下TIPs在竹子中的作用提供證據(jù),為培育抗逆的植物新品種提供新的基因資源。[方法]以毛竹為對(duì)象,利用生物信息學(xué)方法對(duì)毛竹基因組中的TIPs基因進(jìn)行了全面分析,采用實(shí)時(shí)熒光定量PCR技術(shù)分析基因在不同組織及干旱、水淹和Na Cl脅迫下的表達(dá)模式。[結(jié)果]毛竹基因組中含有6個(gè)TIPs同源基因,分別隸屬于3個(gè)亞類(TIP1、TIP2和TIP4)�；蚪Y(jié)構(gòu)預(yù)測(cè)顯示,Pe TIP1;1、Pe TIP1;2、Pe TIP2;2和Pe TIP4;2由2個(gè)外顯子和1個(gè)內(nèi)含子組成,而Pe TIP2;1和Pe TIP4;1由3個(gè)外顯子和2個(gè)內(nèi)含子組成。蛋白結(jié)構(gòu)分析顯示,毛竹6個(gè)TIPs均具有2個(gè)典型的NPA結(jié)構(gòu)域和4個(gè)ar/R模體。通過轉(zhuǎn)錄組數(shù)據(jù)分析基因的組織表達(dá)特異性,結(jié)果表明Pe TIP1;1在各組織中的表達(dá)豐度均較高;Pe TIP1;2主要在花中表達(dá);Pe TIP2;1在根和鞭中表達(dá)量較高;Pe TIP2;2在根中特異表達(dá);Pe TIP4;1在葉片中表達(dá)豐度最高;Pe TIP4;2在筍和鞭中表達(dá)水平較高,在根中最低。實(shí)時(shí)定量PCR結(jié)果分析證明,干旱處理后毛竹根中Pe TIP4;1的表達(dá)量顯著升高(p0.01),Pe TIP2;1、Pe TIP2;2和Pe TIP4;2表達(dá)受到抑制(p0.01);水淹處理后根中Pe TIP1;1和Pe TIP4;1表達(dá)量顯著增加(p0.01),Pe TIP1;2、Pe TIP2;2、和Pe TIP4;2則顯著降低(p0.01);Na Cl處理后根中6個(gè)Pe TIPs的表達(dá)量均顯著增加(p0.01)。干旱處理后毛竹葉片中Pe TIP1;1、Pe TIP1;2和Pe TIP4;1表達(dá)量均顯著增加(p0.01);水淹處理后葉片中Pe TIPs表達(dá)量均顯著提高(p0.01);Na Cl處理后葉片中Pe TIP2;1表達(dá)受到明顯抑制(p0.01)。[結(jié)論]Pe TIPs可能在毛竹抵抗干旱、水淹和Na Cl等非生物脅迫中發(fā)揮著不同程度的作用。
[Abstract]:[Objective] to study the molecular characteristics and expression patterns of TIPs in Phyllostachys pubescens, to provide evidence for revealing the role of TIPs in bamboo under stress stress, and to provide new genetic resources for the cultivation of new stress resistant varieties of Phyllostachys pubescens (Phyllostachys pubescens). [Methods] the TIPs gene in the genome of Phyllostachys pubescens was comprehensively analyzed by bioinformatics, and real-time fluorescence quantitative PCR was used to analyze the gene in different tissues and drought. The expression pattern of water flooded and NaCl stress. [Results: there were 6 TIPs homologous genes in the genome of Phyllostachys pubescens, belonging to three subclasses, TIP1, TIP2 and TIP4, respectively. (1) Pe TIP1; 2Pe TIP2; 2 and Pe TIP4; 2consists of 2 exons and 1 intron, while Pe TIP2; 1 and Pe TIP4; 1 was composed of 3 exons and 2 introns. The 6 TIPs of Phyllostachys pubescens had two typical NPA domains and four ar/R motifs. The specific expression of Pe TIP1 was analyzed by transcriptional data. 1the expression abundance was higher in all tissues; Pe TIP1; (2) mainly expressed in flowers; Pe TIP2; 1High expression in root and whip; Pe TIP2; 2specific expression in roots; Pe TIP4; 1the highest abundance in leaves; Pe TIP4; 2the expression level of Pe TIP4 was higher in bamboo shoot and whip, but lowest in root. The results of real-time quantitative PCR showed that Pe TIP4 in bamboo root after drought treatment; (1) the expression of Pe TIP2 was significantly higher than that of P0. 01 and Pe TIP2; (1) Pe TIP2; 2 and Pe TIP4; 2the expression of P0. 01 was inhibited; Pe TIP1 in roots after waterflooded treatment; 1 and Pe TIP4; (1) the expression of Pe TIP1 increased significantly; 2Pe TIP2; 2, and Pe TIP4; (2) P 0.01 was significantly decreased; The expression of six Pe TIPs in roots increased significantly after NaCl treatment, and Pe TIP1 in leaves of Phyllostachys pubescens after drought treatment. (1) Pe TIP1; 2 and Pe TIP4; (1) the expression of p0.01 was significantly increased; The expression of Pe TIPs in leaves increased significantly after waterflooding treatment. Pe TIP2 in leaves treated with NaCl; 1 the expression of P0. 01 was significantly inhibited. [Conclusion Pe TIPs may play an important role in resistance of Phyllostachys pubescens to drought, flooding and NaCl stress.
【作者單位】： 國(guó)際竹藤中心國(guó)家林業(yè)局竹藤科學(xué)與技術(shù)重點(diǎn)開放實(shí)驗(yàn)室;
【基金】：國(guó)家科技支撐計(jì)劃課題“竹藤優(yōu)異種質(zhì)創(chuàng)制創(chuàng)新與種苗培育標(biāo)準(zhǔn)化示范”(2015BAD04B01) 國(guó)際竹藤中心基本科研業(yè)務(wù)費(fèi)專項(xiàng)資金項(xiàng)目(1632015008)
【分類號(hào)】：S795.7
【正文快照】： 干旱、水淹和鹽脅迫是影響植物生長(zhǎng)、限制作物產(chǎn)量的主要因素。快速調(diào)節(jié)細(xì)胞滲透壓和維持水分平衡是植物應(yīng)對(duì)非生物脅迫的重要方式。維護(hù)細(xì)胞內(nèi)滲透勢(shì)平衡對(duì)保持細(xì)胞內(nèi)穩(wěn)態(tài)至關(guān)重要,這個(gè)過程需要大量的水通道蛋白(Aquaporins,AQPs)調(diào)節(jié)完成[1-2]。AQPs是微生物、植物和動(dòng)物細(xì)

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