本文關(guān)鍵詞：夏眠期仿刺參腸組織實(shí)時(shí)熒光定量PCR分析中內(nèi)參基因的篩選　出處：《中國海洋大學(xué)學(xué)報(bào)(自然科學(xué)版)》2016年07期 　論文類型：期刊論文

  更多相關(guān)文章： 仿刺參 夏眠 內(nèi)參基因 熒光定量PCR

【摘要】：在仿刺參(Apostichopus japonicus)正常時(shí)期、夏眠初期和中期,應(yīng)用實(shí)時(shí)熒光定量PCR技術(shù),檢測CA043、dpolm、ro60、grb2、hiat1、nlrc4、farp1、cyc和gapdh9個(gè)候選內(nèi)參基因在腸組織中的表達(dá)穩(wěn)定性。利用DeltaCt、GeNorm、NormFinder和BestKeeper 4種程序進(jìn)行統(tǒng)計(jì)分析。發(fā)現(xiàn)上述條件中,9個(gè)候選內(nèi)參基因在上述條件中的表達(dá)穩(wěn)定性存在一定差異,不同的軟件處理分析,得到的基因穩(wěn)定性排序不完全一致。綜合4種程序的方法,篩選出基因grb2表達(dá)最穩(wěn)定,可用作內(nèi)參基因,其次為基因ro60及dpolm,而表達(dá)最不穩(wěn)定的是CA043,不適宜作為內(nèi)參基因。本研究為仿刺參中基因表達(dá)定量分析奠定了基礎(chǔ),同時(shí)為仿刺參中內(nèi)參基因的選擇提供一定參考依據(jù)。
[Abstract]:The sea cucumber (Apostichopus japonicus) in normal times, the early and mid-term, real-time fluorescence quantitative PCR technology, application of detection of CA043, dpolm, ro60, Grb2, hiat1, nlrc4, farp1, CYC and gapdh9 expression stability of candidate reference genes in intestinal tissue. By using DeltaCt, GeNorm, NormFinder and BestKeeper 4 program the statistical analysis found. These conditions, there are some differences in the expression stability of 9 candidate reference genes in the above conditions, analysis and treatment of different sort, gene of the stability is not entirely consistent. Methods 4 procedures, screening out the expression of the Grb2 gene in the most stable, can be used as a reference gene, followed by gene ro60 and dpolm, and the expression of the most unstable CA043 is not suitable as a reference gene. This study laid the foundation for the quantitative analysis of gene expression of Apostichopus japonicus, while providing generic thorn ginseng reference gene selection A certain reference.

【作者單位】： 大連海洋大學(xué)農(nóng)業(yè)部北方海水增養(yǎng)殖重點(diǎn)實(shí)驗(yàn)室;中V錆Ｑ蟠笱ШＱ笊鏌糯в胗紙?zhí)育部重甸`笛槭，

本文編號：1400874