不同種類茶葉中揮發(fā)性萜類化合物對映異構(gòu)體研究
發(fā)布時間：2019-05-10 14:18
【摘要】：茶葉中的芳香物質(zhì)即“揮發(fā)性香氣組分(VFC)”,是評價茶葉品質(zhì)的重要指標之一。其中香氣閾值較低的揮發(fā)性萜類化合物是茶葉中最重要的一類香氣成分,呈香上表現(xiàn)為令人愉悅的花果香。茶葉中揮發(fā)性萜類物質(zhì)主要分為單萜類和倍半萜類。萜類物質(zhì)通常存在一對或一對以上的具有不同香氣特征和閾值的對映異構(gòu)體。受以往分析手段的限制,茶葉中揮發(fā)性萜類物質(zhì)對映異構(gòu)體的分布、含量及其對茶葉香氣的具體影響目前尚未明確,限制了人們對茶葉香氣成分的科學(xué)評價。本研究采用頂空固相微萃取-手性氣相色譜質(zhì)譜聯(lián)用技術(shù),建立了茶葉中9種揮發(fā)性萜類化合物的對映異構(gòu)體分析方法,查明了不同茶類及不同品種鮮葉中9種揮發(fā)性萜類化合物的對映異構(gòu)體的分布及含量。結(jié)果如下:1.建立了茶葉中9種揮發(fā)性萜類化合物的對映異構(gòu)體頂空固相微萃取-氣相色譜質(zhì)譜分析方法。各揮發(fā)性萜類化合物對映異構(gòu)體的LOD及LOQ無顯著差異,LOD介于1.27-4.86 ng/g之間,LOQ介于4.24-15.35 ng/g之間;高濃度加標回收率介于83.91%-99.20%之間,RSD值介于8.81%-14.31%之間;低濃度加標回收率介于79.85%-99.78%之間,RSD值介于8.53%-15.28%之間。2.不同茶類中揮發(fā)性萜類化合物對映異構(gòu)體的組成及含量:α-蒎烯僅在正山小種紅茶中被檢測出,構(gòu)型以S-(-)-α-蒎烯為主;芳樟醇氧化物A在綠茶及黑茶中主導(dǎo)構(gòu)型一致,而在部分紅茶及烏龍茶中構(gòu)型相反;芳樟醇氧化物B在烏龍茶中的主導(dǎo)構(gòu)型與綠茶、紅茶、白茶及黑茶中的構(gòu)型相反;芳樟醇氧化物C和D在所有茶樣中的構(gòu)型均保持一致,且大部分僅檢測到單一構(gòu)型;α-松油醇在不同種類的茶葉中對映異構(gòu)體分布各異;4-萜品醇在所有可測得的茶樣中均以R構(gòu)型為主導(dǎo),且僅六堡茶(黑茶)可檢測到S-(+)-4-萜品醇;α-紫羅蘭酮以單一的R構(gòu)型存在于紅茶、烏龍茶、白茶及黑茶中。定量分析結(jié)果表明,芳樟醇在大部分茶樣中都具有最高的含量,平均含量白茶紅茶烏龍茶綠茶黑茶;芳樟醇氧化物B的含量在茶葉中僅次于芳樟醇,平均含量紅茶白茶烏龍茶黑茶綠茶;而其他揮發(fā)性萜類化合物在茶樣中的含量普遍低于100ng/g,且在不同茶類中含量分布迥異。3.不同茶樹品種間揮發(fā)性萜類化合物對映異構(gòu)體的含量差異較大。對23個茶樹品種鮮葉中揮發(fā)性萜類化合物對映異構(gòu)體的定量分析結(jié)果表明,S-(+)-芳樟醇含量介于1961ng/g-13572 ng/g之間,(2S,5R)-芳樟醇氧化物B含量介于1357ng/g-26234 ng/g之間,(2S,5S)-芳樟醇氧化物A含量介于304ng/g-11540 ng/g之間,芳樟醇氧化物D含量介于253.52ng/g-9858.01 ng/g之間,S-(-)-α-松油醇含量介于4.71ng/g-6.98ng/g之間,R-(+)-α-松油醇含量介于4.68ng/g-6.31ng/g之間,S-(+)-4-萜品醇含量介于1.29ng/g-1.73ng/g之間,R-(-)-4-萜品醇含量介于1.25ng/g-1.52ng/g之間,R-Ionone含量介于0.34ng/g-12.21ng/g之間。
[Abstract]:The aromatic substance in tea, "volatile aroma component (VFC)", is one of the important indexes to evaluate the quality of tea. Among them, volatile terpenoids with low aroma threshold are the most important aroma components in tea, showing pleasant flower and fruit aromas. Volatile terpenoids in tea are mainly classified into monoterpenoids and sesquiterpenoids. Terpenoids usually have one or more pairs of enantiomers with different aroma characteristics and threshold values. Limited by previous analytical methods, the distribution and content of enantiomers of volatile terpenoids in tea and their specific effects on tea aroma have not been clearly defined at present, which limits the scientific evaluation of aroma components of tea. In this study, the enantiomer analysis of nine volatile terpenoids in tea was established by headspace solid phase microextraction and chiral gas chromatography-mass spectrometry. The distribution and content of enantiomers of 9 volatile terpenoids in fresh leaves of different teas and different varieties were investigated. The results are as follows: 1. A method for the determination of nine volatile terpenoids in tea by headspace solid phase microextraction-gas chromatography-mass spectrometry (GC-MS) was established. There was no significant difference in LOD and LOQ among the enantiomers of volatile terpenoids. LOD was between 1.27 and 4.86 ng/g, and LOQ was between 4.24 and 15.35 ng/g. The recovery of high concentration standard addition was between 83.91% and 99.20%, and the RSD value was between 8.81% and 14.31%. The recovery of low concentration was between 79.85% and 99.78%, and the RSD was between 8.53% and 15.28%. 2. The composition and content of volatile terpenes enantiomers in different teas: 偽-pinene was detected only in Zhengshan black tea, and the configuration was mainly S-(-)-偽-pinene. The dominant configuration of linalool oxide A in green tea and black tea was the same, but that in some black tea and oolong tea was opposite, and the configuration of linalool oxide B in oolong tea was opposite to that in green tea, black tea, white tea and black tea, and the configuration of linalool oxide B in Oolong tea was opposite to that in green tea, black tea, white tea and black tea. The configuration of linalool oxide C and D in all tea samples remained the same, and most of them only detected a single configuration, the distribution of enantiomers of 偽-terpinol in different kinds of tea was different, and the distribution of enantiomers of 偽-terpinol in different tea samples was different. 4-terpene alcohol is dominated by R configuration in all tea samples, and only S-()-4-terpene alcohol can be detected in Liupu tea (black tea). 偽-ionone exists in black tea, oolong tea, white tea and black tea with a single R configuration. The results of quantitative analysis showed that linalool had the highest content in most tea samples, and the average content was white tea, black tea, oolong tea, green tea, black tea; The content of camphor alcohol oxide B is second only to linalool in tea, and the average content of black tea, white tea, black tea and green tea is second to that of linalool. The content of other volatile terpenoids in tea samples is generally lower than 100 ng / g, and the distribution of other volatile terpenoids in different tea samples is quite different. The content of volatile terpenoids enantiomers varied greatly among different tea varieties. The quantitative analysis of the enantiomers of volatile terpenoids in fresh leaves of 23 tea cultivars showed that the content of S-()-linalool was in the range of 1961ng/g-13572 ng/g, (2S, 2S. 5R)-linalool oxide B content is between 1357ng/g-26234 ng/g and (2S, 5S)-linalool oxide A content is between 304ng/g-11540 ng/g. The contents of camphor alcohol oxide D and S-(-)-偽-terpineol are between 253.52ng/g-9858.01 ng/g and 4.71ng/g-6.98ng/g, respectively. The content of R-()-偽-terpinol is in the range of 4.68ng/g-6.31ng/g, and the content of S-()-4-terpenoid alcohol is in the range of 1.29ng/g-1.73ng/g. The content of R-(-)-4-terpene alcohol is between 1.25ng/g-1.52ng/g and R-Ionone is between 0.34ng/g-12.21ng/g.
【學(xué)位授予單位】：中國農(nóng)業(yè)科學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TS272.7;O657.63
本文編號：2473712
[Abstract]:The aromatic substance in tea, "volatile aroma component (VFC)", is one of the important indexes to evaluate the quality of tea. Among them, volatile terpenoids with low aroma threshold are the most important aroma components in tea, showing pleasant flower and fruit aromas. Volatile terpenoids in tea are mainly classified into monoterpenoids and sesquiterpenoids. Terpenoids usually have one or more pairs of enantiomers with different aroma characteristics and threshold values. Limited by previous analytical methods, the distribution and content of enantiomers of volatile terpenoids in tea and their specific effects on tea aroma have not been clearly defined at present, which limits the scientific evaluation of aroma components of tea. In this study, the enantiomer analysis of nine volatile terpenoids in tea was established by headspace solid phase microextraction and chiral gas chromatography-mass spectrometry. The distribution and content of enantiomers of 9 volatile terpenoids in fresh leaves of different teas and different varieties were investigated. The results are as follows: 1. A method for the determination of nine volatile terpenoids in tea by headspace solid phase microextraction-gas chromatography-mass spectrometry (GC-MS) was established. There was no significant difference in LOD and LOQ among the enantiomers of volatile terpenoids. LOD was between 1.27 and 4.86 ng/g, and LOQ was between 4.24 and 15.35 ng/g. The recovery of high concentration standard addition was between 83.91% and 99.20%, and the RSD value was between 8.81% and 14.31%. The recovery of low concentration was between 79.85% and 99.78%, and the RSD was between 8.53% and 15.28%. 2. The composition and content of volatile terpenes enantiomers in different teas: 偽-pinene was detected only in Zhengshan black tea, and the configuration was mainly S-(-)-偽-pinene. The dominant configuration of linalool oxide A in green tea and black tea was the same, but that in some black tea and oolong tea was opposite, and the configuration of linalool oxide B in oolong tea was opposite to that in green tea, black tea, white tea and black tea, and the configuration of linalool oxide B in Oolong tea was opposite to that in green tea, black tea, white tea and black tea. The configuration of linalool oxide C and D in all tea samples remained the same, and most of them only detected a single configuration, the distribution of enantiomers of 偽-terpinol in different kinds of tea was different, and the distribution of enantiomers of 偽-terpinol in different tea samples was different. 4-terpene alcohol is dominated by R configuration in all tea samples, and only S-()-4-terpene alcohol can be detected in Liupu tea (black tea). 偽-ionone exists in black tea, oolong tea, white tea and black tea with a single R configuration. The results of quantitative analysis showed that linalool had the highest content in most tea samples, and the average content was white tea, black tea, oolong tea, green tea, black tea; The content of camphor alcohol oxide B is second only to linalool in tea, and the average content of black tea, white tea, black tea and green tea is second to that of linalool. The content of other volatile terpenoids in tea samples is generally lower than 100 ng / g, and the distribution of other volatile terpenoids in different tea samples is quite different. The content of volatile terpenoids enantiomers varied greatly among different tea varieties. The quantitative analysis of the enantiomers of volatile terpenoids in fresh leaves of 23 tea cultivars showed that the content of S-()-linalool was in the range of 1961ng/g-13572 ng/g, (2S, 2S. 5R)-linalool oxide B content is between 1357ng/g-26234 ng/g and (2S, 5S)-linalool oxide A content is between 304ng/g-11540 ng/g. The contents of camphor alcohol oxide D and S-(-)-偽-terpineol are between 253.52ng/g-9858.01 ng/g and 4.71ng/g-6.98ng/g, respectively. The content of R-()-偽-terpinol is in the range of 4.68ng/g-6.31ng/g, and the content of S-()-4-terpenoid alcohol is in the range of 1.29ng/g-1.73ng/g. The content of R-(-)-4-terpene alcohol is between 1.25ng/g-1.52ng/g and R-Ionone is between 0.34ng/g-12.21ng/g.
【學(xué)位授予單位】：中國農(nóng)業(yè)科學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TS272.7;O657.63
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