【摘要】：建立超高效液相色譜-串聯(lián)質(zhì)譜儀(UPLC-MS/MS)測(cè)定面制食品中十二烷基苯磺酸鈉含量的新方法。將樣品粉碎后,加入提取劑50%乙醇-水溶液、蛋白沉淀劑1 mol/L乙酸鋅溶液和10.6%亞鐵氰化鉀溶液,超聲提取,定容,微孔濾膜過(guò)濾,用LC-MS/MS進(jìn)行定量分析。以Phenomenex Kintex C_(18)色譜柱為分析柱,甲醇-乙腈為流動(dòng)相進(jìn)行梯度洗脫,采用電噴霧電離源(ESI),以多反應(yīng)監(jiān)測(cè)(MRM)方式進(jìn)行負(fù)離子檢測(cè)。結(jié)果表明:十二烷基苯磺酸鈉在0.5~20.0 mg/kg的范圍內(nèi)線性關(guān)系良好(R~2=0.9985),方法檢出限為0.05 mg/kg,加標(biāo)回收率在87.4%~96.8%之間,相對(duì)標(biāo)準(zhǔn)偏差≤4.5%,完全滿足日常檢測(cè)的要求。該方法前處理簡(jiǎn)單,測(cè)定快速、準(zhǔn)確、靈敏度高,非常適合面制品中十二烷基苯磺酸鈉的定量分析。
[Abstract]:A new method for the determination of sodium dodecylbenzene sulfonate in noodle food by ultra-high performance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) was established. After the sample was pulverized, 50% ethanol aqueous solution, 1 mol/L zinc acetate solution and 10.6% potassium ferrocyanide solution were added as extractant, then ultrasonic extraction, constant volume filtration, micropore filtration and quantitative analysis by LC-MS/MS. Phenomenex Kintex C _ (18) column was used as analytical column, methanol-acetonitrile was used as mobile phase for gradient elution, and (ESI), was used as electrospray ionization source to detect negative ions by multi-reaction monitoring (MRM). The results showed that the linear range of sodium dodecylbenzenesulfonate was 0.5 ~ 20.0 mg/kg (R ~ (2 +) 0.9985), the detection limit of the method was 0. 05 mg/kg, and the recovery rate was between 87. 4% and 96. 8%. Relative standard deviation 鈮，

本文編號(hào)：2408135