【摘要】：目的:運用RRLC-DAD-ESI-Q-TOF-MS技術分析和測定金銀花中主要化學成分,建立山東金銀花多指標定量指紋圖譜。方法:采用RRLC-DAD-ESI-Q-TOF-MS技術對金銀花提取物進行快速分析,根據(jù)獲得的精確相對分子質量和二級質譜碎片信息推斷化合物分子式,結合數(shù)據(jù)庫和文獻對金銀花中化學成分進行鑒別,采用RRLC-DAD法分析測定山東金銀花、河南金銀花和湖南山銀花中主要化學成分含量,在此基礎上建立了山東金銀花多指標定量指紋圖譜。結果:從山東金銀花中檢測到51種化學成分,鑒定了其中32種化學成分;對比測定了山東金銀花、河南金銀花和湖南山銀花中10種主要化學成分(馬錢酸、綠原酸、咖啡酸、馬錢苷、斷氧化馬錢苷、蘆丁、金桃苷、木犀草苷、異綠原酸A和異綠原酸C)的含量;采用指紋圖譜結合相似度分析可以實現(xiàn)不同品種金銀花和湖南山銀花的正確區(qū)分,所得結果與含量測定結果一致。結論:RRLC-DAD-ESIQ-TOF-MS技術分析速度快、靈敏度高、能夠提供化合物及其碎片離子的精確質量信息,可以快速準確地鑒別和測定金銀花中的化學成分。在此基礎上建立的山東道地金銀花的多指標定量指紋圖譜,為山東金銀花質量評價研究提供了技術支持。
[Abstract]:Objective: to analyze and determine the main chemical constituents in Lonicera japonica by RRLC-DAD-ESI-Q-TOF-MS, and to establish quantitative fingerprint of Flos Lonicerae in Shandong Province. Methods: the extract of Flos Lonicerae was analyzed quickly by RRLC-DAD-ESI-Q-TOF-MS, and the molecular formula of the compound was deduced according to the accurate molecular weight and the information of second order mass spectrometry. The chemical constituents of Flos Lonicerae in Shandong Province, Henan Province and Hunan Province were determined by RRLC-DAD method. On this basis, the quantitative fingerprint of honeysuckle in Shandong was established. Results: 51 chemical constituents were detected from Flos Lonicerae in Shandong Province and 32 of them were identified. Ten main chemical constituents (equinic acid, Lv Yuan acid, caffeic acid, riganoside, Rutin, hyperin, luteolin) in Honeysuckle, Henan Flos Lonicerae and Honeysuckle in Hunan Province were determined, and the chemical constituents of Flos Lonicerae, Honeysuckle, Honeysuckle and Honeysuckle in Hunan were determined. Contents of isoLv Yuan acid A and isob_person1# acid C; Fingerprint and similarity analysis can be used to distinguish different varieties of Flos Lonicerae from Honeysuckle in Hunan Province. The results are consistent with the results of content determination. Conclusion: RRLC-DAD-ESIQ-TOF-MS can provide accurate mass information of compounds and their fragments, and can be used to identify and determine the chemical constituents of Flos Lonicerae. On this basis, the quantitative fingerprint of Lonicera japonica in Shandong Province was established, which provided technical support for the quality evaluation of Lonicera japonica in Shandong.
【作者單位】： 山東省中藥質量控制技術重點實驗室山東省分析測試中心;山東中醫(yī)藥大學藥學院;山東農業(yè)大學食品科學與工程學院;濟南三峰生物工程責任有限公司;
【基金】：國家自然科學基金項目(811300700,81473298) 山東省科技發(fā)展計劃項目(2014GSF119031,2016GSF202014) 中央本級重大增減支項目(2060302) 山東省自然基金項目(ZR2013HM075)
【分類號】：O657.63;R284.1

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