【摘要】：目的:建立HPLC法同時(shí)測定鉤吻莖中胡蔓藤堿丙、鉤吻素甲、鉤吻素子、鉤吻素己、鉤吻綠堿和胡蔓藤堿乙的含量,并比較評價(jià)各產(chǎn)地鉤吻生物堿含量的差異。方法:采用外標(biāo)法進(jìn)行測定,色譜柱為ZORBAX Bonus-RP Analytical(4.6 mm×250 mm,5μm),流動(dòng)相為甲醇-0.1%甲酸水梯度洗脫,流速1.0 m L·min~(-1),檢測波長254 nm,柱溫30℃。分別測定32批鉤吻莖,并進(jìn)行聚類分析。結(jié)果:胡蔓藤堿丙、鉤吻素甲、鉤吻素子、鉤吻素己、鉤吻綠堿、胡蔓藤堿乙6個(gè)成分的線性范圍分別為1.00~50.06μg·mL~(-1)(r=1.000 0)、1.13~50.85μg·mL~(-1)(r=0.999 8)、1.16~52.20μg·mL~(-1)(r=1.000 0)、1.16~52.20μg·mL~(-1)(r=0.999 7)、1.06~50.88μg·mL~(-1)(r=0.999 7)和1.01~50.50μg·mL~(-1)(r=1.000 0),平均加樣回收率為93.1%~103.8%(RSD為2.0%~2.9%)。不同產(chǎn)地樣品中鉤吻莖中胡蔓藤堿丙、鉤吻素甲、鉤吻素子、鉤吻素己、鉤吻綠堿和胡蔓藤堿乙含量范圍分別為0.136~2.202、0.564~2.686、0.718~3.553、0.160~2.025、0.151~3.493、0.288~4.700 mg·g~(-1)。結(jié)果顯示不同產(chǎn)地的鉤吻6個(gè)成分含量均有差異,32批不同產(chǎn)地鉤吻藥材可聚為6大類。結(jié)論:該法可為鉤吻多指標(biāo)成分的質(zhì)量評價(jià)提供參考。不同產(chǎn)地鉤吻的各個(gè)化學(xué)成分的含量呈現(xiàn)明顯分類特點(diǎn),為鉤吻地區(qū)鑒定提供依據(jù)。
[Abstract]:Objective: to establish a HPLC method for the simultaneous determination of alkaloids of Gelsemine C, Geptosin A, Gelsemin, Gelsemine and Echinine B in the stems of Gelsemium amurensis, and to compare and evaluate the differences of the contents of the alkaloids of Gelsemine in different areas. Methods: the external standard method was used. The chromatographic column was ZORBAX Bonus-RP Analytical (4.6 mm 脳 250 mm,5 渭 m),) mobile phase was methanol-0.1% formic acid water gradient elution, the flow rate was 1.0 mL min~ (-1), the detection wavelength was 254 nm, column temperature 30 鈩，

本文編號(hào)：2364083