基于DNA折紙模板的金納米顆粒自組裝及其等離子體共振效應(yīng)研究
發(fā)布時(shí)間：2018-11-06 12:46
【摘要】：納米金屬顆粒具有高電子密度、介電特性和催化作用,以及優(yōu)異的生物相容性等優(yōu)勢(shì),在催化、傳感器和生物醫(yī)學(xué)等方面展現(xiàn)出巨大的應(yīng)用潛力。利用“自下而上”的方法在納米級(jí)精度上有序的組裝貴金屬納米顆粒是納米技術(shù)研究的重要方向。DNA“折紙術(shù)”是將天然的DNA長(zhǎng)鏈進(jìn)行反復(fù)折疊,并用短鏈加以固定,由此獲得一系列形狀各異的二維、三維DNA結(jié)構(gòu)。在DNA折紙結(jié)構(gòu)中,每條單鏈的位置都是獨(dú)特的,因此其具有納米級(jí)高度可尋址性,為定位組裝金屬納米顆粒提供了良好的模板。DNA納米技術(shù)為納米顆粒的精確定位,調(diào)控顆粒間相互作用和有效控制納米顆粒自組裝提供了更加豐富的途徑。本文主要利用DNA折紙結(jié)構(gòu)的多樣性和可尋址性,將金屬納米顆粒構(gòu)建成不同的納米結(jié)構(gòu),并對(duì)其法諾性質(zhì)進(jìn)行研究。基于上述內(nèi)容,本論文的研究?jī)?nèi)容主要分為以下三個(gè)方面:1.利用晶種生長(zhǎng)法分別制備了形貌均一,尺寸可控的納米金棒和納米金立方。為了實(shí)現(xiàn)實(shí)驗(yàn)所需求的納米金棒尺寸,通過(guò)調(diào)節(jié)晶種和硝酸銀的加入量,可以獲得不同長(zhǎng)徑比的納米金棒;同樣,通過(guò)調(diào)節(jié)晶種的加入量,獲得目標(biāo)尺寸和形貌均一的納米金立方。金屬納米顆粒的制備,為后續(xù)實(shí)驗(yàn)提供充足的原材料和為研究其光學(xué)性質(zhì)提供有力的保障。2.利用DNA折紙的納米級(jí)可尋址性來(lái)定位組裝納米金棒,形成類似多爾門型的納米金等離子體結(jié)構(gòu)。通過(guò)對(duì)納米金棒DNA全修飾,利用DNA折紙上定位伸出的捕獲鏈與修飾在納米金棒表面的DNA雜交,將金納米棒組裝到DNA折紙上精密加工成多爾門構(gòu)型。采用暗場(chǎng)顯微鏡與掃描電子顯微鏡共定位的方法對(duì)形成的多爾門型等離子體結(jié)構(gòu)研究法諾共振效應(yīng)。3.進(jìn)一步探索以DNA折紙為模板的納米金等離子體結(jié)構(gòu)在法諾共振效應(yīng)的研究。以三角形DNA折紙為模板,精密加工納米金立方和納米金棒形成棒方二聚體,并通過(guò)多種方法表征樣品的結(jié)構(gòu)特征。我們采取暗場(chǎng)顯微鏡與掃描電子顯微鏡共定位的方法對(duì)形成的不同構(gòu)型的棒方二聚體的散射光譜進(jìn)行采集,并研究納米金二聚體的等離子體性質(zhì)。
[Abstract]:Nanometallic particles have many advantages such as high electron density, dielectric properties and catalytic properties, and excellent biocompatibility, showing great potential in catalysis, biosensor and biomedicine. Using "bottom-up" method to assemble noble metal nanoparticles in nanoscale order is an important direction of nanotechnology research. DNA origami is to fold natural DNA long chains repeatedly and fix them with short chains. A series of 2D and 3D DNA structures with different shapes are obtained. In the DNA origami structure, the position of each single strand is unique, so it is highly addressable at nanometer level, which provides a good template for locating and assembling metal nanoparticles. Regulation of particle interaction and effective control of nanoparticles self-assembly provide a more abundant approach. In this paper, the diversity and addressable properties of DNA origami structure were used to construct metal nanoparticles into different nanostructures, and the properties of Fano were studied. Based on the above content, the research content of this paper is divided into the following three aspects: 1. Nanocrystalline gold bars and nanocrystalline gold cubes with uniform morphology and controllable size were prepared by seed growth method. In order to realize the size of nanocrystalline gold rod, the nanocrystalline gold rod with different aspect ratio can be obtained by adjusting the amount of crystal seed and silver nitrate. Similarly, the nanocrystalline gold cubes with uniform target size and morphology were obtained by adjusting the amount of seed added. The preparation of metal nanoparticles provides sufficient raw materials for subsequent experiments and provides a strong guarantee for the study of their optical properties. 2. Nano-level addressable properties of DNA origami paper were used to locate and assemble nanocrystalline gold rods to form a door-like nanocrystalline gold plasma structure. The gold nanorods were fabricated on DNA origami by fully modifying the gold nanorods with DNA, and the trapping chains located and extended on the DNA origami were hybridized with the DNA modified on the surface of the nanorods, and the gold nanorods were assembled into DNA origami. The Farno resonance effect was studied by means of colocation of dark field microscope and scanning electron microscope. Further study on the resonance effect of nanocrystalline gold plasma with DNA origami as template was carried out. Using triangular DNA origami as template, nanocrystalline gold cubes and nanocrystalline gold rods were fabricated to form rod-square dimers, and the structural characteristics of the samples were characterized by various methods. The scattering spectra of nanocrystalline gold dimers with different configurations were collected by co-localization of dark field microscope and scanning electron microscope and the plasma properties of nanocrystalline gold dimers were studied.
【學(xué)位授予單位】：南京郵電大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TB383.1;O614.123
本文編號(hào)：2314321
[Abstract]:Nanometallic particles have many advantages such as high electron density, dielectric properties and catalytic properties, and excellent biocompatibility, showing great potential in catalysis, biosensor and biomedicine. Using "bottom-up" method to assemble noble metal nanoparticles in nanoscale order is an important direction of nanotechnology research. DNA origami is to fold natural DNA long chains repeatedly and fix them with short chains. A series of 2D and 3D DNA structures with different shapes are obtained. In the DNA origami structure, the position of each single strand is unique, so it is highly addressable at nanometer level, which provides a good template for locating and assembling metal nanoparticles. Regulation of particle interaction and effective control of nanoparticles self-assembly provide a more abundant approach. In this paper, the diversity and addressable properties of DNA origami structure were used to construct metal nanoparticles into different nanostructures, and the properties of Fano were studied. Based on the above content, the research content of this paper is divided into the following three aspects: 1. Nanocrystalline gold bars and nanocrystalline gold cubes with uniform morphology and controllable size were prepared by seed growth method. In order to realize the size of nanocrystalline gold rod, the nanocrystalline gold rod with different aspect ratio can be obtained by adjusting the amount of crystal seed and silver nitrate. Similarly, the nanocrystalline gold cubes with uniform target size and morphology were obtained by adjusting the amount of seed added. The preparation of metal nanoparticles provides sufficient raw materials for subsequent experiments and provides a strong guarantee for the study of their optical properties. 2. Nano-level addressable properties of DNA origami paper were used to locate and assemble nanocrystalline gold rods to form a door-like nanocrystalline gold plasma structure. The gold nanorods were fabricated on DNA origami by fully modifying the gold nanorods with DNA, and the trapping chains located and extended on the DNA origami were hybridized with the DNA modified on the surface of the nanorods, and the gold nanorods were assembled into DNA origami. The Farno resonance effect was studied by means of colocation of dark field microscope and scanning electron microscope. Further study on the resonance effect of nanocrystalline gold plasma with DNA origami as template was carried out. Using triangular DNA origami as template, nanocrystalline gold cubes and nanocrystalline gold rods were fabricated to form rod-square dimers, and the structural characteristics of the samples were characterized by various methods. The scattering spectra of nanocrystalline gold dimers with different configurations were collected by co-localization of dark field microscope and scanning electron microscope and the plasma properties of nanocrystalline gold dimers were studied.
【學(xué)位授予單位】：南京郵電大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TB383.1;O614.123
【參考文獻(xiàn)】
相關(guān)期刊論文 前2條
1 楊洋;柳華杰;劉冬生;;DNA納米機(jī)器[J];化學(xué)進(jìn)展;2008年Z1期
2 ;Analogic China map constructed by DNA[J];Chinese Science Bulletin;2006年24期
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