熒光碳量子點(diǎn)的合成及其在分析檢測(cè)中的應(yīng)用
發(fā)布時(shí)間：2018-11-03 19:29
【摘要】：碳量子點(diǎn)由于其優(yōu)良的光學(xué)性能、良好的生物相容性和低細(xì)胞毒性等優(yōu)點(diǎn),被廣泛應(yīng)用于分析檢測(cè)、環(huán)境監(jiān)測(cè)、生物成像、藥物傳遞與釋放、疾病的診斷和治療等領(lǐng)域。為進(jìn)一步擴(kuò)大其應(yīng)用范圍,尤其是在生物醫(yī)學(xué)方面的應(yīng)用,必須不斷改進(jìn)合成方法和技術(shù),制備出強(qiáng)熒光性能、高熒光量子產(chǎn)率、低毒性以及良好的生物相容性的碳量子點(diǎn),并深入研究其發(fā)光機(jī)理,為應(yīng)用提供充足的理論依據(jù)。近年來(lái),農(nóng)藥及獸藥殘留、藥品的制假售假、食品添加劑超標(biāo)等問(wèn)題不斷涌現(xiàn),嚴(yán)重威脅到人類(lèi)的生活和身體健康。因此,針對(duì)常見(jiàn)藥物分子和食品添加劑建立一種可靠、快速、靈敏的檢測(cè)方法刻不容緩。基于以上問(wèn)題,本論文主要開(kāi)展了以下工作:以L-精氨酸為碳源,采用水熱法一步合成了熒光碳量子點(diǎn)(C-dots),并對(duì)其合成條件進(jìn)行了簡(jiǎn)單的考察,通過(guò)紫外吸收光譜、熒光光譜、傅立葉變換紅外光譜以及X射線光電子能譜等手段對(duì)其進(jìn)行了表征分析�；诙喟桶�(DA)對(duì)C-dots的熒光具有明顯的猝滅作用,設(shè)計(jì)了檢測(cè)多巴胺的熒光探針。且當(dāng)濃度在0.50-100.00μM范圍內(nèi)時(shí),碳點(diǎn)的熒光猝滅程度與多巴胺的濃度呈良好的線性關(guān)系,相關(guān)系數(shù)R=0.9995,檢出限低至93 nM。對(duì)市售鹽酸多巴胺注射液和健康成人的尿樣中多巴胺進(jìn)行檢測(cè),得到回收率和精密度(RSD)范圍分別為100.29%-104.21%和0.47%-5.42%。以梔子花為碳源,采用一步水熱法制備了具有強(qiáng)藍(lán)色熒光的碳量子點(diǎn)溶液,并利用多種表征手段對(duì)其性質(zhì)進(jìn)行了分析。利用甲硝唑(MNZ)能導(dǎo)致合成碳點(diǎn)發(fā)生熒光猝滅現(xiàn)象,且在濃度為2.50-225.00μM范圍內(nèi),其熒光猝滅程度與甲硝唑的濃度呈良好的線性關(guān)系,相關(guān)系數(shù)R=0.9971,檢出限為279 nM。對(duì)市售的兩種品牌的甲硝唑片進(jìn)行檢測(cè)時(shí),得到回收率和精密度(RSD)分別為91.32%-104.37%和0.44%-1.20%,結(jié)果令人滿(mǎn)意。以蘆薈為碳源,采用水熱法一步合成了具有黃綠色熒光的碳量子點(diǎn)溶液。并采用多種手段對(duì)其進(jìn)行了分析表征。以C-dots為熒光分子,檸檬黃(TZ)為猝滅劑,基于二者間靜態(tài)猝滅的原理建立了簡(jiǎn)便、快速檢測(cè)檸檬黃的熒光探針。在濃度為0.25-32.50μM范圍內(nèi),熒光猝滅程度與檸檬黃濃度呈良好的線性關(guān)系,相關(guān)系數(shù)R=0.9993,檢出限低至73 nM。將該方法應(yīng)用于食品樣品中檸檬黃的檢測(cè),得到日內(nèi)和日間回收率分別為88.56%-103.74%和87.27%-106.60%,日內(nèi)和日間精密度(RSD)分別為0.18%-3.90%和0.37%-5.82%。
[Abstract]:Due to its excellent optical properties, good biocompatibility and low cytotoxicity, carbon quantum dots are widely used in analytical detection, environmental monitoring, biological imaging, drug delivery and release, disease diagnosis and treatment. In order to further expand its application, especially in biomedical applications, it is necessary to continuously improve the synthetic methods and techniques to prepare carbon quantum dots with strong fluorescence properties, high fluorescence quantum yield, low toxicity and good biocompatibility. The luminescence mechanism is studied in depth to provide sufficient theoretical basis for application. In recent years, pesticide and veterinary drug residues, drug counterfeiting and sale, food additives over the standard and other problems continue to emerge, seriously threatening human life and health. Therefore, it is urgent to establish a reliable, rapid and sensitive detection method for common drug molecules and food additives. Based on the above problems, the main work of this thesis is as follows: fluorescent carbon quantum dots (C-dots) were synthesized by hydrothermal method using L-arginine as carbon source. Fluorescence spectra, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize them. Based on the obvious quenching effect of dopamine (DA) on the fluorescence of C-dots, a fluorescence probe for the detection of dopamine was designed. When the concentration was in the range of 0.50-100.00 渭 M, the fluorescence quenching degree of carbon point showed a good linear relationship with the concentration of dopamine. The correlation coefficient was 0.9995, and the detection limit was as low as 93 nM.. The recovery and precision (RSD) range of dopamine hydrochloride injection and healthy adult urine samples were 100.29-104.21% and 0.47-5.42%, respectively. Carbon quantum dots with strong blue fluorescence were prepared by one-step hydrothermal method with gardenia as carbon source and their properties were analyzed by various characterization methods. Using metronidazole (MNZ) can lead to fluorescence quenching of synthetic carbon point, and the fluorescence quenching degree of metronidazole is linear with the concentration of metronidazole in the range of 2.50-225.00 渭 M. the correlation coefficient is 0.9971, and the fluorescence quenching degree of metronidazole is linear to that of metronidazole in the range of 2.50-225.00 渭 M. Detection limit is 279 nM. The recovery and precision of metronidazole tablets were 91.32 -104.37% and 0.44 -1.20%, respectively. The results were satisfactory. The solution of carbon quantum dots with yellowish green fluorescence was synthesized by hydrothermal method using aloe vera as carbon source. It was characterized by various methods. Using C-dots as fluorescence molecule and lemon yellow (TZ) as quenching agent, a simple and rapid fluorescence probe for the detection of lemon yellow was established based on the principle of static quenching between the two. In the concentration range of 0.25-32.50 渭 M, the fluorescence quenching degree showed a good linear relationship with the concentration of lemon yellow. The correlation coefficient was 0.9993, and the detection limit was as low as 73 nM.. The method was applied to the determination of lemon yellow in food samples. The recoveries were 88.56% -103.74% and 87.27% -106.60%, respectively. The intra-day and inter-day precision (RSD) were 0.18- 3.90% and 0.37-5.82%, respectively.
【學(xué)位授予單位】：西華師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：O657.3
本文編號(hào)：2308765
[Abstract]:Due to its excellent optical properties, good biocompatibility and low cytotoxicity, carbon quantum dots are widely used in analytical detection, environmental monitoring, biological imaging, drug delivery and release, disease diagnosis and treatment. In order to further expand its application, especially in biomedical applications, it is necessary to continuously improve the synthetic methods and techniques to prepare carbon quantum dots with strong fluorescence properties, high fluorescence quantum yield, low toxicity and good biocompatibility. The luminescence mechanism is studied in depth to provide sufficient theoretical basis for application. In recent years, pesticide and veterinary drug residues, drug counterfeiting and sale, food additives over the standard and other problems continue to emerge, seriously threatening human life and health. Therefore, it is urgent to establish a reliable, rapid and sensitive detection method for common drug molecules and food additives. Based on the above problems, the main work of this thesis is as follows: fluorescent carbon quantum dots (C-dots) were synthesized by hydrothermal method using L-arginine as carbon source. Fluorescence spectra, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize them. Based on the obvious quenching effect of dopamine (DA) on the fluorescence of C-dots, a fluorescence probe for the detection of dopamine was designed. When the concentration was in the range of 0.50-100.00 渭 M, the fluorescence quenching degree of carbon point showed a good linear relationship with the concentration of dopamine. The correlation coefficient was 0.9995, and the detection limit was as low as 93 nM.. The recovery and precision (RSD) range of dopamine hydrochloride injection and healthy adult urine samples were 100.29-104.21% and 0.47-5.42%, respectively. Carbon quantum dots with strong blue fluorescence were prepared by one-step hydrothermal method with gardenia as carbon source and their properties were analyzed by various characterization methods. Using metronidazole (MNZ) can lead to fluorescence quenching of synthetic carbon point, and the fluorescence quenching degree of metronidazole is linear with the concentration of metronidazole in the range of 2.50-225.00 渭 M. the correlation coefficient is 0.9971, and the fluorescence quenching degree of metronidazole is linear to that of metronidazole in the range of 2.50-225.00 渭 M. Detection limit is 279 nM. The recovery and precision of metronidazole tablets were 91.32 -104.37% and 0.44 -1.20%, respectively. The results were satisfactory. The solution of carbon quantum dots with yellowish green fluorescence was synthesized by hydrothermal method using aloe vera as carbon source. It was characterized by various methods. Using C-dots as fluorescence molecule and lemon yellow (TZ) as quenching agent, a simple and rapid fluorescence probe for the detection of lemon yellow was established based on the principle of static quenching between the two. In the concentration range of 0.25-32.50 渭 M, the fluorescence quenching degree showed a good linear relationship with the concentration of lemon yellow. The correlation coefficient was 0.9993, and the detection limit was as low as 73 nM.. The method was applied to the determination of lemon yellow in food samples. The recoveries were 88.56% -103.74% and 87.27% -106.60%, respectively. The intra-day and inter-day precision (RSD) were 0.18- 3.90% and 0.37-5.82%, respectively.
【學(xué)位授予單位】：西華師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：O657.3
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相關(guān)期刊論文 前4條
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