【摘要】：通過化學(xué)分析和生物活性評(píng)價(jià)考察丹參藥材的品質(zhì)差異,探討丹參抗血小板聚集生物活性的主要貢獻(xiàn)成分。采用高效液相色譜(HPLC)技術(shù)建立丹參藥材HPLC指紋圖譜,以抗血小板聚集相對(duì)效價(jià)作為指標(biāo),評(píng)價(jià)不同產(chǎn)地不同批次丹參藥材的品質(zhì)差異,構(gòu)建基于化學(xué)表征及生物效價(jià)測(cè)定的評(píng)價(jià)模式。結(jié)果表明,不同批次丹參藥材的HPLC指紋圖譜相似度很高(相似度0.930~0.998),而其抗血小板聚集相對(duì)效價(jià)相差10倍,提示化學(xué)指紋圖譜難以反映丹參的活性和質(zhì)量差異。通過化學(xué)指紋圖譜與抗血小板聚集生物效價(jià)進(jìn)行譜效相關(guān)分析,篩選出與生物活性相關(guān)系數(shù)大于0.5的6個(gè)色譜峰:二氫丹參酮Ⅰ、隱丹參酮、丹參酮Ⅰ、丹參酮ⅡA及2個(gè)未知化合物。對(duì)上述4種已知化合物單體進(jìn)行活性驗(yàn)證發(fā)現(xiàn),隱丹參酮的抗血小板聚集活性最強(qiáng),而其它3種丹參酮類化合物幾乎沒有體外抗血小板聚集活性。進(jìn)一步比較丹參中高含量成分丹酚酸B與低含量成分隱丹參酮的活性貢獻(xiàn),結(jié)果表明,兩者的活性貢獻(xiàn)基本相當(dāng),說明隱丹參酮是丹參中低含量高活性成分,對(duì)評(píng)價(jià)丹參質(zhì)量具有重要貢獻(xiàn)度。
[Abstract]:The quality difference of Salvia miltiorrhiza was investigated by chemical analysis and bioactivity evaluation, and the main contribution components of Salvia miltiorrhiza against platelet aggregation were discussed. The HPLC fingerprint of Salvia miltiorrhiza was established by (HPLC), and the quality of different batches of Salvia miltiorrhiza was evaluated by using the relative titer of anti-platelet aggregation as an index. An evaluation model based on chemical characterization and bioassay was established. The results showed that the HPLC fingerprint of different batches of Salvia miltiorrhiza had high similarity (0.930 ~ 0.998), but the relative titer of anti-platelet aggregation was 10 times different, suggesting that the chemical fingerprint could not reflect the difference of activity and quality of Salvia miltiorrhiza. Six chromatographic peaks, dihydrotanshinone 鈪，

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