金納米簇?zé)晒忖缧吞结樃哽`敏檢測(cè)精胺
發(fā)布時(shí)間:2018-07-31 08:34
【摘要】:以紫脲酸銨(Murexide,MU)為還原劑及保護(hù)劑,采用水熱合成法,合成了紫脲酸銨保護(hù)的熒光金納米簇(MU-Au NCs),合成方法簡(jiǎn)單、快速;诰穼(duì)MU-Au NCs的熒光猝滅現(xiàn)象,建立了快速、超靈敏檢測(cè)精胺的"Turn off"型熒光分析方法,在優(yōu)化的條件下,本方法檢測(cè)精胺的線(xiàn)性范圍為0.003~300μmol/L,檢測(cè)限為1 nmol/L(S/N=3)。本方法為構(gòu)建精胺生物傳感器及實(shí)際樣品檢測(cè)提供了理論基礎(chǔ)和參考。
[Abstract]:MU-Au NCs), was synthesized by hydrothermal synthesis with Murexide MU as reductant and protectant. The synthesis method of MU-Au NCs), was simple and rapid. Based on the fluorescence quenching of spermine to MU-Au NCs, a rapid and hypersensitive method for the detection of spermine by "Turn off" fluorescence analysis was developed. Under the optimized conditions, the linear range of detection of spermine was 0.003 渭 mol / L and the detection limit was 1 nmol/L (S/N=3). This method provides a theoretical basis and reference for the construction of spermine biosensor and the actual sample detection.
【作者單位】: 西北師范大學(xué)生命科學(xué)學(xué)院;甘肅省生物電化學(xué)與環(huán)境分析重點(diǎn)實(shí)驗(yàn)室;
【基金】:國(guó)家自然科學(xué)基金項(xiàng)目(Nos.21165015,21565020)資助~~
【分類(lèi)號(hào)】:O657.3;TS207.3
本文編號(hào):2155024
[Abstract]:MU-Au NCs), was synthesized by hydrothermal synthesis with Murexide MU as reductant and protectant. The synthesis method of MU-Au NCs), was simple and rapid. Based on the fluorescence quenching of spermine to MU-Au NCs, a rapid and hypersensitive method for the detection of spermine by "Turn off" fluorescence analysis was developed. Under the optimized conditions, the linear range of detection of spermine was 0.003 渭 mol / L and the detection limit was 1 nmol/L (S/N=3). This method provides a theoretical basis and reference for the construction of spermine biosensor and the actual sample detection.
【作者單位】: 西北師范大學(xué)生命科學(xué)學(xué)院;甘肅省生物電化學(xué)與環(huán)境分析重點(diǎn)實(shí)驗(yàn)室;
【基金】:國(guó)家自然科學(xué)基金項(xiàng)目(Nos.21165015,21565020)資助~~
【分類(lèi)號(hào)】:O657.3;TS207.3
【相似文獻(xiàn)】
相關(guān)期刊論文 前3條
1 朱青;氚標(biāo)記精胺的制備[J];原子能科學(xué)技術(shù);1978年01期
2 王新星;于朝磊;馬靜;常青;;高效液相色譜法測(cè)定鹵蟲(chóng)無(wú)節(jié)幼體中精胺含量[J];漁業(yè)科學(xué)進(jìn)展;2013年06期
3 李剛;方團(tuán)團(tuán);胡忠陽(yáng);葉明立;;離子色譜法測(cè)定環(huán)境水樣中腐胺、尸胺、組胺、精胺、亞精胺[J];環(huán)境化學(xué);2014年05期
,本文編號(hào):2155024
本文鏈接:http://sikaile.net/kejilunwen/huaxue/2155024.html
最近更新
教材專(zhuān)著