【摘要】：目的建立柿Diospyros kaki葉的HPLC指紋圖譜,并進(jìn)行多成分測定,為柿葉及其相關(guān)產(chǎn)品的質(zhì)量控制提供科學(xué)依據(jù)。方法采用HPLC法,Diamonsil C18(250 mm×4.6 mm,5μm)色譜柱;以乙腈-0.2%甲酸水為流動相梯度洗脫;體積流量1.0 m L/min;柱溫35℃;檢測波長360 nm;進(jìn)樣量10μL;對22批柿葉藥材進(jìn)行指紋圖譜研究,用中藥色譜指紋圖譜相似度評價(jià)系統(tǒng)(2004A版)進(jìn)行評價(jià),系統(tǒng)聚類分析和PCA分析將22批柿葉藥材分為兩類,同時(shí)對金絲桃苷、異槲皮苷、槲皮素和山柰素成分測定方法進(jìn)行方法學(xué)考察。結(jié)果建立了柿葉的指紋圖譜,共標(biāo)定了12個(gè)色譜峰為柿葉藥材指紋圖譜的特征峰,金絲桃苷在0.044~0.880μg,異槲皮苷在0.078~1.560μg,槲皮素在0.040~0.800μg,山柰素在0.035~0.700μg線性關(guān)系良好,r均大于0.999 8,平均回收率分別為97.59%、98.97%、100.55%、99.96%。結(jié)論 HPLC指紋圖譜結(jié)合多成分測定可為柿葉及其相關(guān)產(chǎn)品的質(zhì)量控制提供參考。
[Abstract]:Objective to establish the HPLC fingerprint of persimmon Diospyros kaki leaves and to determine the multicomponent in order to provide scientific basis for the quality control of persimmon leaves and related products. Methods Diamonsil C18 (250mm 脳 4.6mm ~ 5 渭 m) column, acetonitrile-0.2% formic acid water as mobile phase gradient elution, volumetric flow rate 1.0 mL / min, column temperature 35 鈩，

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