氮摻雜的熒光碳點的化學(xué)發(fā)光及其應(yīng)用研究
發(fā)布時間：2018-07-22 15:16
【摘要】：化學(xué)發(fā)光法因其不需要光源,靈敏度高,線性范圍寬和儀器簡單等優(yōu)點,已成為最有發(fā)展?jié)摿Φ姆治龇椒ㄖ弧＝陙?隨著納米技術(shù)的不斷發(fā)展,化學(xué)發(fā)光已從傳統(tǒng)的分子體系擴展到納米體系,從而極大地改善了化學(xué)發(fā)光的靈敏度并拓寬了其應(yīng)用范圍。氮摻雜的熒光碳點(NFCDs)作為一種由碳元素和氮元素組成的非金屬納米材料,具有良好的熒光性,優(yōu)異的分散性和化學(xué)穩(wěn)定性,以及較低的毒性等特點。目前,NFCDs主要作為熒光探針用于生物和環(huán)境檢測方面,因此探究NFCDs參與的化學(xué)發(fā)光新體系具有極大的理論意義和應(yīng)用價值。本研究工作中,以抗壞血酸和氨水為原料,通過簡單的一步超聲法合成NFCDs,并探究了其在新的化學(xué)發(fā)光體系中的作用。建立了三種基于NFCDs的流動注射化學(xué)發(fā)光的新方法,主要研究內(nèi)容如下:1.在酸性介質(zhì)中,KMn O4/Ce(IV)直接氧化NFCDs產(chǎn)生相對強的化學(xué)發(fā)光,硫脲/單寧的加入對KMnO_4-NFCDs體系的化學(xué)發(fā)光有明顯的增敏作用,而對Ce(IV)-NFCDs體系的化學(xué)發(fā)光幾乎沒有影響,由此建立了用KMnO_4-NFCDs體系測定硫脲/單寧的新方法。在最佳實驗條件下,測定硫脲和單寧的線性范圍分別為1.0×10 7 1.0×10 5 mol/L和7.0×10 8 7.0×10 6 mol/L,檢出限分別為3.8×10 8 mol/L和2.6×10 8 mol/L。將該方法分別用于橘汁和橘皮中硫脲含量的測定,以及五倍子中單寧含量的測定,獲得滿意的結(jié)果。2.Ce(IV)和Na_2SO_3反應(yīng)產(chǎn)生微弱的化學(xué)發(fā)光,NFCDs對該體系的發(fā)光信號有顯著的放大作用,加入吲哚乙酸后,Ce(IV)-Na_2SO_3-NFCDs體系的化學(xué)發(fā)光得到明顯的抑制。由此建立了一種分析水樣中痕量吲哚乙酸的新方法,標(biāo)準(zhǔn)曲線的濃度范圍為2.0×10-8 1.0×10-6 mol/L,檢出限為5.6×10-9 mol/L。3.在堿性條件下,NFCDs能明顯增敏KMnO_4-luminol體系的化學(xué)發(fā)光,加入Mn~(2+)后,KMnO_4-luminol-NFCDs體系的化學(xué)發(fā)光強度得到明顯的抑制。在最優(yōu)的實驗條件下,Mn~(2+)在3.0×10-7 5.0×10-5 mol/L濃度范圍內(nèi)與其化學(xué)發(fā)光的抑制值呈線性關(guān)系,檢出限為4.3×10-8 mol/L。將該方法用于水樣中Mn~(2+)含量的測定。
[Abstract]:Chemiluminescence has become one of the most promising analytical methods due to its advantages of no light source, high sensitivity, wide linear range and simple instrument. In recent years, with the development of nanotechnology, chemiluminescence has been extended from traditional molecular system to nano-system, which greatly improves the sensitivity of chemiluminescence and widens its application scope. Nitrogen-doped fluorescent carbon dots (NFCDs), as a nonmetallic nano-material composed of carbon and nitrogen elements, have good fluorescence, excellent dispersion and chemical stability, and low toxicity. Currently, NFCDs are mainly used as fluorescent probes for biological and environmental detection. Therefore, it is of great theoretical significance and practical value to explore the new chemiluminescence system in which NFCDs participate. In this study, ascorbic acid and ammonia were used as raw materials to synthesize NFCDs by a simple one-step ultrasonic method, and the role of NFCDs in the new chemiluminescence system was investigated. Three new flow injection chemiluminescence methods based on NFCDs were established. The main research contents are as follows: 1. In acidic medium, direct oxidation of NFCDs by KMnO4 / ce (IV) produces relatively strong chemiluminescence. The addition of thiourea / tannin can obviously increase the chemiluminescence of KMnO4-NFCDs system, but has little effect on the chemiluminescence of ce (IV) -NFCDs system. A new method for the determination of thiourea / tannin by KMnO4-NFCDs system was established. Under the optimum conditions, the linear ranges of thiourea and tannin were 1.0 脳 10 ~ (7) and 1.0 脳 10 ~ (5) mol / L, respectively, and the detection limits were 3.8 脳 10 ~ (8) mol / L and 2.6 脳 10 ~ (8) mol 路L ~ (-1), respectively. The method was applied to the determination of thiourea content in orange juice and orange peel, and tannin content in gallnut, respectively. The satisfactory results were obtained. 2. Ce (IV) and Na _ 2SO _ 3 reaction produced weak chemiluminescent NFCDs to amplify the luminescence signal of the system. The chemiluminescence of ce (IV) -Na _ 2SO _ 2SO _ 3-NFCDs system was obviously inhibited after indoleacetic acid was added. A new method for the determination of trace indoleacetic acid in water samples was established. The concentration range of the standard curve was 2.0 脳 10 ~ (-8) ~ (-1) 脳 10 ~ (-6) mol / L, and the detection limit was 5.6 脳 10 ~ (-9) mol / L ~ (-3). NFCDs can obviously sensitize the chemiluminescence of KMnO4-luminol system under alkaline condition, and the chemiluminescence intensity of KMnO4-luminol-NFCDs system can be inhibited obviously by adding Mn2 into KMnO4-luminol-NFCDs system. Under the optimum experimental conditions, there is a linear relationship between the chemiluminescence inhibition value and the concentration range of 3.0 脳 10 ~ (-7) 渭 mol / L ~ (-5) 脳 10 ~ (-5) mol 路L ~ (2). The detection limit is 4.3 脳 10 ~ (-8) mol 路L ~ (-1). The method has been applied to the determination of Mn2 in water samples.
【學(xué)位授予單位】：山西師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3
本文編號：2137877
[Abstract]:Chemiluminescence has become one of the most promising analytical methods due to its advantages of no light source, high sensitivity, wide linear range and simple instrument. In recent years, with the development of nanotechnology, chemiluminescence has been extended from traditional molecular system to nano-system, which greatly improves the sensitivity of chemiluminescence and widens its application scope. Nitrogen-doped fluorescent carbon dots (NFCDs), as a nonmetallic nano-material composed of carbon and nitrogen elements, have good fluorescence, excellent dispersion and chemical stability, and low toxicity. Currently, NFCDs are mainly used as fluorescent probes for biological and environmental detection. Therefore, it is of great theoretical significance and practical value to explore the new chemiluminescence system in which NFCDs participate. In this study, ascorbic acid and ammonia were used as raw materials to synthesize NFCDs by a simple one-step ultrasonic method, and the role of NFCDs in the new chemiluminescence system was investigated. Three new flow injection chemiluminescence methods based on NFCDs were established. The main research contents are as follows: 1. In acidic medium, direct oxidation of NFCDs by KMnO4 / ce (IV) produces relatively strong chemiluminescence. The addition of thiourea / tannin can obviously increase the chemiluminescence of KMnO4-NFCDs system, but has little effect on the chemiluminescence of ce (IV) -NFCDs system. A new method for the determination of thiourea / tannin by KMnO4-NFCDs system was established. Under the optimum conditions, the linear ranges of thiourea and tannin were 1.0 脳 10 ~ (7) and 1.0 脳 10 ~ (5) mol / L, respectively, and the detection limits were 3.8 脳 10 ~ (8) mol / L and 2.6 脳 10 ~ (8) mol 路L ~ (-1), respectively. The method was applied to the determination of thiourea content in orange juice and orange peel, and tannin content in gallnut, respectively. The satisfactory results were obtained. 2. Ce (IV) and Na _ 2SO _ 3 reaction produced weak chemiluminescent NFCDs to amplify the luminescence signal of the system. The chemiluminescence of ce (IV) -Na _ 2SO _ 2SO _ 3-NFCDs system was obviously inhibited after indoleacetic acid was added. A new method for the determination of trace indoleacetic acid in water samples was established. The concentration range of the standard curve was 2.0 脳 10 ~ (-8) ~ (-1) 脳 10 ~ (-6) mol / L, and the detection limit was 5.6 脳 10 ~ (-9) mol / L ~ (-3). NFCDs can obviously sensitize the chemiluminescence of KMnO4-luminol system under alkaline condition, and the chemiluminescence intensity of KMnO4-luminol-NFCDs system can be inhibited obviously by adding Mn2 into KMnO4-luminol-NFCDs system. Under the optimum experimental conditions, there is a linear relationship between the chemiluminescence inhibition value and the concentration range of 3.0 脳 10 ~ (-7) 渭 mol / L ~ (-5) 脳 10 ~ (-5) mol 路L ~ (2). The detection limit is 4.3 脳 10 ~ (-8) mol 路L ~ (-1). The method has been applied to the determination of Mn2 in water samples.
【學(xué)位授予單位】：山西師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3
【參考文獻】
相關(guān)期刊論文 前1條
1 曾強;朱浩波;朱紅艷;朱小利;戴昌雄;鄧淳;何瑜;張修華;宋功武;;氮摻雜碳點的制備及在六價鉻檢測中的應(yīng)用[J];化學(xué)與生物工程;2016年03期
，本文編號：2137877
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