【摘要】：系統(tǒng)地比較了3種常用的離子化技術(shù)電噴霧電離(ESI)、大氣壓化學(xué)電離(APCI)、大氣壓光致電離(APPI)對(duì)脂類化合物的離子化效率、檢測(cè)靈敏度和覆蓋范圍,以探討多重離子化液相色譜-質(zhì)譜(LC-MS)方法在血清脂質(zhì)組學(xué)研究中的適用性。血清樣本經(jīng)甲基叔丁基醚萃取后,采用Ascentiss Express C_8色譜柱(150 mm×2.1 mm,2.7μm)和二元線性梯度洗脫分離,流動(dòng)相(A)為乙腈-水(3:2,V/V,含0.1%甲酸,10 mmol/L甲酸銨),B為異丙醇-乙腈(9:1,V/V,含0.1%甲酸,10 mmol/L甲酸銨),分別采用ESI、APCI和APPI離子源正、負(fù)離子模式進(jìn)行質(zhì)譜檢測(cè)。結(jié)果表明,ESI離子源對(duì)脂肪酸類、甘油脂類、甘油磷脂類化合物、鞘磷脂類化合物的離子化效率最高,對(duì)異戊烯醇脂類化合物的離子化效率與APPI離子源相當(dāng),APPI離子源對(duì)膽固醇(酯)類化合物的檢測(cè)靈敏度最高,APCI離子源對(duì)各類化合物的檢測(cè)靈敏度均低于ESI或APPI離子源;采用ESI和APPI離子源相結(jié)合的LC-MS脂質(zhì)組學(xué)分析方法可以提高分析方法的整體靈敏度和血清中脂類信息檢測(cè)的完整性。
[Abstract]:The ionization efficiency, sensitivity and coverage of 3 kinds of ionized electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) for lipid compounds were compared systematically to explore the applicability of multiple ionization liquid chromatography mass spectrometry (LC-MS) in serum liposome study. After the extraction of methyl tert butyl ether, the Ascentiss Express C_8 column (150 mm x 2.1 mm, 2.7 u m) and two element linear gradient elution were used. The mobile phase (A) was acetonitrile water (3:2, V/V, 0.1% formic acid, 10 mmol/L formate), and B was isopropanol acetonitrile (9:1, 0.1% formic acid, 10 ammonium formate), respectively. The results showed that the ionization efficiency of ESI ion sources was the highest for fatty acids, glycerol lipids, glycerol phospholipids and sphingomyelin compounds, and the ionization efficiency of isoamyl alcohol lipids was equal to that of APPI ion sources, and the sensitivity of APPI ion sources to cholesterol (ester) compounds was the highest, AP The detection sensitivity of CI ion sources for all kinds of compounds is lower than that of ESI or APPI ion sources. The LC-MS liposome analysis method combined with ESI and APPI ion sources can improve the overall sensitivity of the analytical method and the integrity of the detection of lipid information in the serum.
【作者單位】： 中國(guó)醫(yī)學(xué)科學(xué)院/北京協(xié)和醫(yī)學(xué)院藥物研究所天然藥物活性物質(zhì)與功能國(guó)家重點(diǎn)實(shí)驗(yàn)室;中央民族大學(xué)生命與環(huán)境科學(xué)學(xué)院生物成像與系統(tǒng)生物學(xué)研究中心;中國(guó)醫(yī)學(xué)科學(xué)院/北京協(xié)和醫(yī)學(xué)院北京協(xié)和醫(yī)院;
【基金】：國(guó)家高技術(shù)研究發(fā)展計(jì)劃863項(xiàng)目(No.2014AA021101)資助~~
【分類號(hào)】：R446.1;O657.63
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本文編號(hào)：2137824