本文選題：稀土銪配合物 + BSA�。� 參考：《寧夏大學》2017年碩士論文

【摘要】：設計合成了四種含磷多功能配體及稀土銪配合物,采用核磁共振、紅外光譜、元素分析、差熱-熱重、摩爾比法及計算化學進行輔助的一系列方法對合成的配體及相應配合物的結構與組成進行了表征和分析,結果表明,四種配體與銪離子均以1:1形式結合。分別使用紫外可見光譜法和熒光光譜法研究了四種銪配合物與牛血清白蛋白在不同溫度下的相互作用。由紫外光譜可知:合成的四種銪配合物與牛血清白蛋白之間均有較強的結合能力,其結合力大小為:Eu(pic)3L3-BSAEu(pic)3L2-BSAEu(pic)3L1-BSAEuL4-BSA;由熒光光譜可知:合成的四種銪配合物與牛血清白蛋白內(nèi)源熒光猝滅是以靜態(tài)猝滅的方式進行的,并計算了在不同溫度下四種銪配合物與牛血清白蛋白相關的熱力學參數(shù)(AH,δS,AG)、結合常數(shù)K和結合位點n。結果表明,四種銪配合物均可以自發(fā)進行(δG0),且均為放熱反應(δH0),體系的作用力類型是范德華力和氫鍵(AS0、AH0)。使用熒光光譜法研究了 Cu2+和Fe3+在不同溫度下對合成的四種銪配合物與牛血清白蛋白之間相互作用的影響。研究結果表明:在Cu2+和Fe3+的作用下,大大增強了銪配合物與牛血清白蛋白的結合能力,說明兩種金屬離子參與且對銪配合物與牛血清白蛋白的結合有一定的影響。分別通過紫外光譜法與熒光光譜法對四種銪配合物與DNA的相互作用模式進行了研究。紫外光譜表明:加入DNA探針后,銪配合物的紫外吸收光譜圖的最大吸收峰均出現(xiàn)了一定的減色效應且其最大吸收波長輕微的藍移,其結合常數(shù)分別為5.34×105 L mol-1、2.15×105 L·mol-1、2.57×105 L·mol-1和2.28×105 L·ol-1與報道中的數(shù)值接近,我們初步推測四種銪配合物均插入DNA與其發(fā)生嵌插作用。熒光光譜表明:四種銪配合物與DNA的結合常數(shù)分別為4.11×105 L·mol-1、4.50×105 L·mol-1、9.39×105L·mol-1 和 5.0×105L·mol-1 與文獻報道的數(shù)值接近,我們判斷四種銪配合物對EB-DNA復合體系的熒光猝滅可能為靜態(tài)猝滅,進一步推測四種銪配合物均可以與DNA以經(jīng)典嵌插進行結合。使用抑菌圈法研究了四種銪配合物對大腸桿菌和金黃色葡萄球菌的抑菌活性,結果表明四種合成的銪配合物對大腸桿菌和金黃色葡萄球菌均有一定的抑菌性能。
[Abstract]:Four kinds of phosphorus-containing multifunctional ligands and rare earth europium complexes were designed and synthesized. Nuclear magnetic resonance (NMR), infrared spectroscopy (IR), elemental analysis, differential thermogravimetry (DTA) were used. The structure and composition of the synthesized ligands and their corresponding complexes were characterized and analyzed by molar ratio method and a series of methods assisted by computational chemistry. The results showed that the four ligands combined with europium ions in the form of 1:1. The interaction of four europium complexes with bovine serum albumin at different temperatures was studied by UV-Vis and fluorescence spectra. The UV spectra show that the four europium complexes have strong binding ability to bovine serum albumin (BSA). The binding force of EU (pic) 3L3-BSAEu (pic) 3L2-BSAEu (pic) 3L1-BSAEuL4-BSA-BSA-BSA-BSA-BSA-BSA-BSA-BSA-BSA-BSA-BSA.The fluorescence spectra show that the four europium complexes have been quenched with bovine serum albumin (BSA) by static quenching. The thermodynamic parameters of four europium complexes related to bovine serum albumin (AH, 未 Szag), binding constant K and binding site n were calculated at different temperatures. The results show that all of the four europium complexes can spontaneously proceed (未 G _ 0), and all of them are exothermic reactions (未 H _ 0). The interaction forces of the system are van der Waals force and hydrogen bond (as _ 0C _ 0 AH _ 0). The effects of Cu _ 2 and Fe _ 3 on the interaction of four europium complexes with bovine serum albumin (BSA) at different temperatures were studied by fluorescence spectroscopy. The results show that the binding ability of europium complexes with bovine serum albumin is greatly enhanced by Cu _ 2 and Fe _ 3, indicating that two kinds of metal ions are involved and have certain influence on the binding of europium complexes to bovine serum albumin. The interaction modes of four europium complexes with DNA were studied by UV and fluorescence spectra respectively. UV spectra showed that the maximum absorption peaks of europium complexes showed a decreasing color effect and a slight blue shift of the maximum absorption wavelength after the addition of DNA probe. The binding constants are 5.34 脳 105L mol-1C 2.15 脳 105L mol-1C 2.57 脳 105L mol-1 and 2.28 脳 105L ol-1, respectively. We preliminarily speculate that the four europium complexes are intercalated. The fluorescence spectra showed that the binding constants of the four europium complexes were 4.11 脳 105L mol-1 (4.50 脳 105L mol ~ (-1) mol-1 and 5.0 脳 105L mol-1 respectively, which were close to those reported in the literature. We concluded that the fluorescence quenching of EB-DNA complex by four europium complexes might be static quenching. It is further speculated that the four europium complexes can bind to DNA by classical intercalation. The bacteriostatic activity of four europium complexes against Escherichia coli and Staphylococcus aureus was studied by using bacteriostasis method. The results showed that the four synthesized europium complexes had certain bacteriostasis to Escherichia coli and Staphylococcus aureus.
【學位授予單位】：寧夏大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O641.4

【參考文獻】

相關期刊論文 前10條

1 史蕾;楊文聰;曾淑瑩;莫婷婷;張召;曹曼麗;劉海洋;;咔咯鈷(Ⅲ)配合物與DNA的相互作用及抗腫瘤活性[J];高等學校化學學報;2016年06期

2 陳永杰;邢貞方;曹爽;王瑩;;Synthesis and luminescent properties of terbium complex with 2-amino-4-chlorobenzoic acid[J];Journal of Rare Earths;2016年03期

3 劉向榮;孫秀超;楊再文;趙順省;楊水蘭;閆森;;2-呋喃甲醛-4-羥基苯甲酰腙及其Cu(Ⅱ)配合物的晶體結構及與CT-DNA的結合性能[J];無機化學學報;2016年02期

4 張東;鄧月義;;2-羥基-4-甲氧基二苯甲酮稀土配合物光轉換材料的合成與光致發(fā)光性能[J];稀土;2015年06期

5 劉偉;楊水蘭;宋盼;楊天林;;一種含磷三足體衍生物及其銪配合物的合成及性能表征[J];應用化學;2015年07期

6 佘文潔;劉偉;張瑤;楊天林;;N-二(苯-二氨基甲�；�)甲基磷酸及其銪(Ⅲ)配合物的合成及在DNA電化學傳感器中的應用[J];中國稀土學報;2014年05期

7 高繼紅;楊天林;;酰胺型開鏈冠醚類稀土配合物的合成及其與DNA的作用研究[J];中國稀土學報;2014年04期

8 王滿元;張超;李靜;李朝霞;龔慕辛;;青蒿截瘧組合物與牛血清白蛋白的相互作用[J];高等學校化學學報;2014年02期

9 丁應濤;王立衡;鄭成鳳;蔣淑戀;黃立漳;高飛;汪慶祥;;一種席夫堿鈷(Ⅱ)配合物的合成及其與DNA的相互作用[J];化學試劑;2013年04期

10 白菊;王興明;趙娜;;酪氨酸-鉺(Ⅲ)配合物與鯡魚精DNA的作用方式研究[J];四川大學學報(自然科學版);2013年02期

相關碩士學位論文 前1條

1 馬樹芝;稀土雜環(huán)類配合物的合成、表征及其生物活性研究[D];上海師范大學;2008年

，

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