本文選題：黑豆皮花青素 + 純化�。� 參考：《河南工業(yè)大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年06期

【摘要】：以黑豆皮為原料,采用超聲波輔助法提取3種黑豆皮花青素,利用高效液相色譜法對(duì)其進(jìn)行定性定量分析,然后用兩種不同型號(hào)的制備型色譜柱分離純化含量最高的黑豆皮花青素,并鑒定其純度。結(jié)果表明:3種黑豆皮花青素中的主要成分是矢車(chē)菊素-3-O-葡萄糖苷(C_3G),其中東北黑豆皮花青素中C_3G含量最高,為959.7 mg/100 g,其次是山西黑豆皮花青素。用兩種色譜柱純化東北黑豆皮花青素制備C_3G,色譜柱1 C18(77 mm×450 mm,10~100μm)分離的C_3G純度為77.2%,色譜柱2 C18(19 mm×150 mm,5.0μm)分離的C_3G純度為97.0%,色譜柱2分離的C_3G可以代替標(biāo)品,適合實(shí)驗(yàn)室小量制備并做后續(xù)性質(zhì)的研究。
[Abstract]:Three kinds of anthocyanins from black soybean peel were extracted by ultrasonic assisted method and analyzed qualitatively and quantitatively by high performance liquid chromatography (HPLC). Then the anthocyanins with the highest content were separated and purified by two different types of preparative chromatographic columns and the purity of anthocyanins was identified. The results showed that the main component of the three kinds of anthocyanins of black soybean peel was cornulin -3-O- glucoside C _ 3G _ (1), in which the content of C _ (3G) was the highest (959.7 mg/100 / g) in the anthocyanin of black soybean peel in Northeast China, followed by the anthocyanin of black bean peel in Shanxi Province. C3G was purified by two kinds of chromatographic columns. The purity of C3G was 77.2% for C _ (18) C _ (18) ~ (77) mm 脳 450 mm ~ (10 ~ (10) ~ (100 渭 m) for C ~ (3G), and 97.0 渭 m for C _ (3G) separation on column 2 C _ (18) ~ 19 mm 脳 150 mm ~ (5.0 渭 m). The C3G separated by column 2 could replace the standard product. Suitable for laboratory preparation and follow-up study.
【作者單位】： 河南工業(yè)大學(xué)糧油食品學(xué)院;
【基金】：國(guó)家“十三五”重點(diǎn)研發(fā)計(jì)劃專(zhuān)項(xiàng)課題(2016YFD0400203) 河南工業(yè)大學(xué)“省屬高�；究蒲袠I(yè)務(wù)費(fèi)專(zhuān)項(xiàng)資金”項(xiàng)目(2014YWJC06) 河南省谷物資源轉(zhuǎn)化與利用重點(diǎn)實(shí)驗(yàn)室開(kāi)放課題(PL2017009)
【分類(lèi)號(hào)】：O652.63;TS264.4

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